Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,757,006	G→A	A437T (GCT→ACT)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,757,006	0	G	A	100.0%	210.3 / NA	65	A437T (GCT→ACT)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/31);  total (34/31)

TCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCG  >  NC_000913/1756884‑1757140                                                                                                                           |                                                                                                                                       tCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCt                                                                                                                        >  2:394613/1‑139 (MQ=255)    tGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGc                                                                                                                     >  1:1156735/1‑139 (MQ=255)            accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCagag                                                                                                                              <  2:1355273/122‑1 (MQ=255)            accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCagag                                                                                                                              >  1:1355273/1‑122 (MQ=255)            accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                             >  1:923645/1‑139 (MQ=255)            accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                             >  1:1272012/1‑139 (MQ=255)            accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                             >  1:1283665/1‑139 (MQ=255)            accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCCGCAGAGCGGTc                                                                                                                         <  1:207718/127‑1 (MQ=255)            accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCCGCAGAGCGGTc                                                                                                                         >  2:207718/1‑127 (MQ=255)             ccaccaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAgg                                                                                                            <  2:1283665/139‑1 (MQ=255)              caccaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGt                                                                                                           <  1:394613/139‑1 (MQ=255)               accaACGAAGAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTg                                                                                                          <  2:1156735/139‑1 (MQ=255)                                     aGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                    <  2:699335/139‑1 (MQ=255)                                               gAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAGCTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTa                                                                          >  2:1286801/1‑139 (MQ=255)                                               gAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                    >  2:4433/1‑129 (MQ=255)                                               gAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                    <  1:4433/129‑1 (MQ=255)                                                 gCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTAcc                                                                        >  2:1427363/1‑139 (MQ=255)                                                  cAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAGCTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCg                                                                       <  1:1286801/139‑1 (MQ=255)                                                    aaGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAg                                                                     <  2:923645/139‑1 (MQ=255)                                                     aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACaa                                                                                                               <  1:780487/96‑1 (MQ=255)                                                     aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACaa                                                                                                               >  2:780487/1‑96 (MQ=255)                                                     aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                                                                                                      >  2:919214/1‑105 (MQ=255)                                                     aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                                                                                                      <  1:919214/105‑1 (MQ=255)                                                     aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGc                                                                    >  2:327395/1‑139 (MQ=255)                                                       gCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCgg                                                                  <  1:1427363/139‑1 (MQ=255)                                                        cGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGc                                                                 <  2:990379/139‑1 (MQ=255)                                                        cGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGc                                                                 <  1:327395/139‑1 (MQ=255)                                                                   aGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAacac                                                      <  1:453309/139‑1 (MQ=255)                                                                       tGTTAAAGAGATCACTTATACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCa                                                  >  1:829504/1‑139 (MQ=255)                                                                               agaTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCagag                                                                                                                              <  1:227461/55‑1 (MQ=255)                                                                               agaTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCagag                                                                                                                              >  2:227461/1‑55 (MQ=255)                                                                                 agcACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCagag                                                                                                                              <  1:290132/51‑1 (MQ=255)                                                                                 aTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCagag                                                                                                                              >  2:290132/1‑53 (MQ=255)                                                                                 aTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGACTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                    >  2:1120127/1‑95 (MQ=255)                                                                                 aTCACTTCGACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                    <  1:1120127/95‑1 (MQ=255)                                                                                   cACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                    <  2:1540731/93‑1 (MQ=255)                                                                                   cACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                    >  1:1540731/1‑93 (MQ=255)                                                                                      ttCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTg                                   >  1:1367428/1‑139 (MQ=255)                                                                                                gatTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCactact                                                           >  2:485610/1‑105 (MQ=255)                                                                                                gatTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCactact                                                           <  1:485610/105‑1 (MQ=255)                                                                                                gatTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCt                         >  1:285394/1‑139 (MQ=255)                                                                                                gatTGCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCt                         >  2:983363/1‑139 (MQ=255)                                                                                                   ttCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTgg                                                                                                                      >  1:1299652/1‑43 (MQ=255)                                                                                                   ttCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTgg                                                                                                                      <  2:1299652/43‑1 (MQ=255)                                                                                                   ttCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTg                                                                                                          >  2:731175/1‑55 (MQ=255)                                                                                                   ttCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTg                                                                                                          <  1:731175/55‑1 (MQ=255)                                                                                                   ttCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCtttt                      >  1:752139/1‑139 (MQ=255)                                                                                                           tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGtt                                                                                               >  2:273710/1‑58 (MQ=255)                                                                                                           tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGt                                                                                          <  2:128643/63‑1 (MQ=255)                                                                                                           tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGt                                                                                          >  1:128643/1‑63 (MQ=255)                                                                                                           tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAg                                                                     <  2:117151/84‑1 (MQ=255)                                                                                                           tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAg                                                                     >  1:117151/1‑84 (MQ=255)                                                                                                           tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAata                              >  2:63528/1‑123 (MQ=255)                                                                                                           tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAata                              <  1:63528/123‑1 (MQ=255)                                                                                                           tCTGAGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGtt                                                                                               <  1:273710/58‑1 (MQ=255)                                                                                                             tGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                    >  1:958504/1‑67 (MQ=255)                                                                                                             tGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                    <  2:714514/67‑1 (MQ=255)                                                                                                             tGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                    <  2:958504/67‑1 (MQ=255)                                                                                                             tGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                    >  1:714514/1‑67 (MQ=255)                                                                                                             tGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTg                                                                                  >  2:1061787/1‑69 (MQ=255)                                                                                                             tGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTg                                                                                  <  1:1061787/69‑1 (MQ=255)                                                                                                             tGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaattaat            >  1:146761/1‑139 (MQ=255)                                                                                                                    aGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGtata     <  1:983363/139‑1 (MQ=255)                                                                                                                      aaCTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATc   >  1:1082005/1‑139 (MQ=255)                                                                                                                       aCTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCg  <  2:752139/139‑1 (MQ=255)                                                                                                                           |                                                                                                                                       TCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCG  >  NC_000913/1756884‑1757140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑