Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,841,897 G→A *136* (TAG→TAA
T80I (ACT→ATT) 
nudG →
ynjH ←
CTP pyrophosphohydrolase, also hydrolyzes 2‑hydroxy‑dATP, 8‑hydroxy‑dGTP, 5‑hydroxy‑CTP, dCTP and 5‑methyl‑dCTP
DUF1496 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,841,8970GA100.0% 208.0 / NA 64*136* (TAG→TAA
T80I (ACT→ATT) 
nudG
ynjH
CTP pyrophosphohydrolase, also hydrolyzes 2‑hydroxy‑dATP, 8‑hydroxy‑dGTP, 5‑hydroxy‑CTP, dCTP and 5‑methyl‑dCTP
DUF1496 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/33);  total (31/33)

CGTTACAGGCACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAA  >  NC_000913/1841764‑1842028
                                                                                                                                     |                                                                                                                                   
cGTTACAGGCACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTa                                                                                                                                <  2:422755/139‑1 (MQ=255)
 gTTACAGGCACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTaa                                                                                                                               <  2:372216/139‑1 (MQ=255)
           caTGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtc                                                                                                                     <  2:639455/139‑1 (MQ=255)
            aTGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtca                                                                                                                    >  1:45450/1‑139 (MQ=255)
                                        aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                        <  1:539029/139‑1 (MQ=255)
                                        aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                        <  1:1422871/139‑1 (MQ=255)
                                               gagGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCttt                                                                                 <  2:45450/139‑1 (MQ=255)
                                                  gcgcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCAc                                                                                                                <  1:756540/105‑1 (MQ=255)
                                                  gcgcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCAc                                                                                                                >  2:756540/1‑105 (MQ=255)
                                                    gcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTa                                                                            >  1:1677125/1‑139 (MQ=255)
                                                                  tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                        >  1:1469233/1‑113 (MQ=255)
                                                                  tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                        <  2:1469233/113‑1 (MQ=255)
                                                                  tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCttt                                                                                 >  1:1489803/1‑120 (MQ=255)
                                                                  tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCttt                                                                                 <  2:1489803/120‑1 (MQ=255)
                                                                  tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGaa                                                              <  2:1290208/139‑1 (MQ=255)
                                                                  tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGaa                                                              >  2:1486310/1‑139 (MQ=255)
                                                                    gCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCg                                                                                      <  1:416780/113‑1 (MQ=255)
                                                                    gCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCg                                                                                      >  2:416780/1‑113 (MQ=255)
                                                                              aCATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTaa                                                                                                                               >  2:145132/1‑62 (MQ=255)
                                                                              aCATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTaa                                                                                                                               <  1:145132/62‑1 (MQ=255)
                                                                                   ccATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGttt                                                                                                                         <  2:674418/63‑1 (MQ=255)
                                                                                   ccATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGttt                                                                                                                         >  1:674418/1‑63 (MQ=255)
                                                                                            gagGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTgg                                                                                                           <  1:1450074/68‑1 (MQ=255)
                                                                                            gagGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTgg                                                                                                           >  2:1450074/1‑68 (MQ=255)
                                                                                              ggCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCt                                                                                                             >  2:925178/1‑64 (MQ=255)
                                                                                              ggCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCt                                                                                                             >  2:1304222/1‑64 (MQ=255)
                                                                                              ggCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCt                                                                                                             <  1:925178/64‑1 (MQ=255)
                                                                                              ggCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCt                                                                                                             <  1:1304222/64‑1 (MQ=255)
                                                                                                 gTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTg                                                                                                                       <  1:1372089/51‑1 (MQ=255)
                                                                                                 gTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTg                                                                                                                       >  2:1372089/1‑51 (MQ=255)
                                                                                                  tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCt                                                                                                             <  1:1461450/60‑1 (MQ=255)
                                                                                                  tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCt                                                                                                             >  2:1461450/1‑60 (MQ=255)
                                                                                                  tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcagc                                                                                                  >  2:289654/1‑71 (MQ=255)
                                                                                                  tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcagc                                                                                                  <  1:289654/71‑1 (MQ=255)
                                                                                                  tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                        <  2:660763/81‑1 (MQ=255)
                                                                                                  tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                        >  1:660763/1‑81 (MQ=255)
                                                                                                  tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCtt                                                                   <  1:1201270/102‑1 (MQ=255)
                                                                                                  tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCtt                                                                   >  2:1201270/1‑102 (MQ=255)
                                                                                                  tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGt                              >  1:605554/1‑139 (MQ=255)
                                                                                                  tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGt                              >  1:261307/1‑139 (MQ=255)
                                                                                                   ttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtcatc                                                                                                                  >  1:741541/1‑54 (MQ=255)
                                                                                                   ttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtcatc                                                                                                                  <  2:741541/54‑1 (MQ=255)
                                                                                                   ttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcag                                                                                                   >  1:661993/1‑69 (MQ=255)
                                                                                                   ttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcag                                                                                                   <  2:661993/69‑1 (MQ=255)
                                                                                                    tATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGa                                                     <  2:722464/114‑1 (MQ=255)
                                                                                                    tATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGa                                                     >  1:722464/1‑114 (MQ=255)
                                                                                                       ggCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAgg                         >  2:158626/1‑139 (MQ=255)
                                                                                                        gCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAggg                        <  1:1323763/139‑1 (MQ=255)
                                                                                                         cTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGc                                                                                                              >  1:381686/1‑52 (MQ=255)
                                                                                                         cTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGc                                                                                                              <  2:381686/52‑1 (MQ=255)
                                                                                                            tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                        >  2:1557684/1‑71 (MQ=255)
                                                                                                            tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCtt                                                                                        <  1:1557684/71‑1 (MQ=255)
                                                                                                             aCGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTg                                                      <  2:175651/104‑1 (MQ=255)
                                                                                                             aCGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTg                                                      >  1:175651/1‑104 (MQ=255)
                                                                                                                  ccgccAGACCAGCGGATTAATGCTAAGGGTTTTGtcat                                                                                                                   <  1:607924/38‑1 (MQ=255)
                                                                                                                  ccgccAGACCAGCGGATTAATGCTAAGGGTTTTGtcat                                                                                                                   >  2:607924/1‑38 (MQ=255)
                                                                                                                  ccgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCtt                                                                                  <  2:200264/71‑1 (MQ=255)
                                                                                                                  ccgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCtt                                                                                  >  1:200264/1‑71 (MQ=255)
                                                                                                                   cgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCtt                                                                   >  2:831453/1‑85 (MQ=255)
                                                                                                                   cgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCtt                                                                   <  1:831453/85‑1 (MQ=255)
                                                                                                                             gCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCttt                                                                                 >  1:177350/1‑61 (MQ=255)
                                                                                                                             gCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCttt                                                                                 <  2:177350/61‑1 (MQ=255)
                                                                                                                              cGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaa  <  2:261307/139‑1 (MQ=255)
                                                                                                                              cGGATTAATGCTAAGGGTTTTGTCATCACGCTCGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaa  >  2:1003332/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                   
CGTTACAGGCACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAA  >  NC_000913/1841764‑1842028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: