Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	183,215	C→T	G251G (GGC→GGT)	cdaR →	carbohydrate diacid regulon transcriptional regulator, autoregulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	183,215	0	C	T	100.0%	183.8 / NA	57	G251G (GGC→GGT)	cdaR	carbohydrate diacid regulon transcriptional regulator, autoregulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/32);  total (25/32)

ATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGAT  >  NC_000913/183087‑183331                                                                                                                                 |                                                                                                                     aTGGTGGTGTTTAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTa                                                                                                            <  2:1442543/139‑1 (MQ=255) aTGGTGGTGTTGAAACCGGCGTTGAACTC‑TTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTAc                                                                                                           >  1:325888/1‑139 (MQ=255)      ggtgTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGt                                                                                                       <  2:600943/139‑1 (MQ=255)             aaaCCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCa                                                                                                >  1:736893/1‑139 (MQ=255)                     gTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                        <  2:903499/139‑1 (MQ=255)                     gTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                        <  1:175536/139‑1 (MQ=255)                     gTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                        <  2:39173/139‑1 (MQ=255)                           ctctTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGt                                                                                                       <  1:727918/118‑1 (MQ=255)                           ctctTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGt                                                                                                       >  2:727918/1‑118 (MQ=255)                                 tGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGt                                                                            <  1:556229/139‑1 (MQ=255)                                        tGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGa                                                                     >  2:1478585/1‑139 (MQ=255)                                         gggATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGaa                                                                    <  2:797887/139‑1 (MQ=255)                                                 gaagaTCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTGTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAAcgacga                                                            <  2:232407/139‑1 (MQ=255)                                                   agaTCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTATGGCGAAAACGACGAtg                                                          <  2:325888/139‑1 (MQ=255)                                                       catcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                        <  1:20323/105‑1 (MQ=255)                                                       catcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                        >  2:20323/1‑105 (MQ=255)                                                         tcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGt                                                                                                       >  2:1200946/1‑88 (MQ=255)                                                         tcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGt                                                                                                       <  1:1200946/88‑1 (MQ=255)                                                         tcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAAcgacga                                                            <  2:273476/131‑1 (MQ=255)                                                         tcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAAcgacga                                                            >  1:273476/1‑131 (MQ=255)                                                         tcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtggtg                                                    >  2:57203/1‑139 (MQ=255)                                                                  aGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAg                                           <  2:736893/139‑1 (MQ=255)                                                                   gTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCg                                                                                                         >  2:1176898/1‑76 (MQ=255)                                                                   gTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCg                                                                                                         <  1:1176898/76‑1 (MQ=255)                                                                   gTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAAcgacga                                                            <  2:278667/121‑1 (MQ=255)                                                                   gTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAAcgacga                                                            >  1:278667/1‑121 (MQ=255)                                                                    ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAAAGACGATGGTGGTGGGTAAACAGCg                                         >  1:24394/1‑139 (MQ=255)                                                                            cTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAacgacg                                                             <  2:653083/111‑1 (MQ=255)                                                                            cTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAacgacg                                                             >  1:653083/1‑111 (MQ=255)                                                                               aTTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGa                                                                                     <  1:150886/84‑1 (MQ=255)                                                                               aTTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGa                                                                                     >  2:150886/1‑84 (MQ=255)                                                                               aTTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTAc                                                                          >  1:231950/1‑95 (MQ=255)                                                                               aTTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTAc                                                                          <  2:231950/95‑1 (MQ=255)                                                                                    ccGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGt                                                                            >  1:1594088/1‑88 (MQ=255)                                                                                    ccGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGt                                                                            <  2:1594088/88‑1 (MQ=255)                                                                                      gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCg                                         >  1:181869/1‑121 (MQ=255)                                                                                      gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCg                                         <  2:181869/121‑1 (MQ=255)                                                                                              gagTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTAttttt               <  1:1374062/139‑1 (MQ=255)                                                                                                 tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc            >  2:1689474/1‑139 (MQ=255)                                                                                                  aCGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                        <  1:73505/62‑1 (MQ=255)                                                                                                  aCGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                        >  2:73505/1‑62 (MQ=255)                                                                                                     gCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCgctg                       >  1:801090/1‑124 (MQ=255)                                                                                                     gCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCgctg                       <  2:801090/124‑1 (MQ=255)                                                                                                      ccAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAacgacg                                                             <  1:1152629/85‑1 (MQ=255)                                                                                                      ccAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAacgacg                                                             >  2:1152629/1‑85 (MQ=255)                                                                                                      ccAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAAcgacga                                                            >  2:348020/1‑86 (MQ=255)                                                                                                      ccAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAAcgacga                                                            <  1:348020/86‑1 (MQ=255)                                                                                                      ccAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGAt       <  1:1689474/139‑1 (MQ=255)                                                                                                           tGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGaa                                                                    <  2:270879/73‑1 (MQ=255)                                                                                                           tGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGaa                                                                    >  1:270879/1‑73 (MQ=255)                                                                                                           tGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGAt  <  1:57203/139‑1 (MQ=255)                                                                                                               ttttCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCgg                                        <  1:58123/97‑1 (MQ=255)                                                                                                               ttttCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCgg                                        >  2:58123/1‑97 (MQ=255)                                                                                                                       tttCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTa                                                >  2:1569418/1‑81 (MQ=255)                                                                                                                       tttCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTa                                                <  1:1569418/81‑1 (MQ=255)                                                                                                                         tCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAacgacg                                                             <  2:1560714/66‑1 (MQ=255)                                                                                                                         tCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAacgacg                                                             >  1:1560714/1‑66 (MQ=255)                                                                                                                                 |                                                                                                                     ATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGAT  >  NC_000913/183087‑183331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑