Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,892,402	A→G	S278S (AGT→AGC)	yoaA ←	putative ATP‑dependent helicase, DinG family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,892,402	0	A	G	100.0%	224.4 / NA	63	S278S (AGT→AGC)	yoaA	putative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/32);  total (31/32)

CGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGA  >  NC_000913/1892270‑1892515                                                                                                                                     |                                                                                                                  cGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTgg                                                                                                             >  2:1642956/1‑139 (MQ=255)   ccACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGc                                                                                                           <  2:545392/139‑1 (MQ=255)      cGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAg                                                                                                        >  2:233675/1‑139 (MQ=255)           tAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGAt                                                                                                   >  2:1268982/1‑139 (MQ=255)                 aGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                             >  2:253744/1‑139 (MQ=255)                 aGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                             <  2:1669007/139‑1 (MQ=255)                   ttCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGct                                                                                                                 <  1:1474143/117‑1 (MQ=255)                   ttCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGct                                                                                                                 >  2:1474143/1‑117 (MQ=255)                   ttCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCAc                                                                                           >  1:622657/1‑139 (MQ=255)                      cAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACttt                                                                                        <  1:756715/139‑1 (MQ=255)                      cAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGCTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACttt                                                                                        >  2:1103200/1‑139 (MQ=255)                         ggTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACTGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGt                                                                                     <  2:157008/139‑1 (MQ=255)                          gtgtCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTa                                                                                    <  1:614730/139‑1 (MQ=255)                            gtCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAAc                                                                                  <  1:253744/139‑1 (MQ=255)                                     cAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTggg                                                                           <  1:747419/137‑1 (MQ=255)                                     cAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTggg                                                                           >  2:747419/1‑137 (MQ=255)                                        tAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGct                                                                                                                 >  1:23134/1‑96 (MQ=255)                                        tAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAActgct                                                                              <  2:482413/131‑1 (MQ=255)                                        tAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAActgct                                                                              >  1:482413/1‑131 (MQ=255)                                              tGCCCGCTGAATTTGCGGATTAGCTACCAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGCGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                             >  1:315983/1‑110 (MQ=255)                                              tGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                             <  2:315983/110‑1 (MQ=255)                                                 ccGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCg                                                             <  1:1268982/139‑1 (MQ=255)                                                  cGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcg                                                                                              <  2:548803/105‑1 (MQ=255)                                                  cGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcg                                                                                              >  1:548803/1‑105 (MQ=255)                                                            gCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGc                                                  >  2:1336025/1‑139 (MQ=255)                                                             cGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCg                                                 <  1:1336025/139‑1 (MQ=255)                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcg                                                                                              >  1:344488/1‑79 (MQ=255)                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcg                                                                                              <  2:344488/79‑1 (MQ=255)                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCtttt                                  >  2:482193/1‑139 (MQ=255)                                                                            cTCACGCAGGTTACCACGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCtttt                                  >  2:1132981/1‑139 (MQ=255)                                                                              cACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                             >  1:143509/1‑78 (MQ=255)                                                                              cACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                             <  2:143509/78‑1 (MQ=255)                                                                                       tACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGct                                                                                                                 >  2:853630/1‑49 (MQ=255)                                                                                       tACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGct                                                                                                                 <  1:853630/49‑1 (MQ=255)                                                                                        aCCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTgg                                                                            >  1:1609/1‑85 (MQ=255)                                                                                        aCCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTgg                                                                            <  2:1609/85‑1 (MQ=255)                                                                                               tAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCaa                                                                                                         >  2:288467/1‑49 (MQ=255)                                                                                               tAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCaa                                                                                                         <  1:288467/49‑1 (MQ=255)                                                                                               tAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGAt                                                                                                   <  1:1316885/55‑1 (MQ=255)                                                                                               tAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGAt                                                                                                   >  2:1316885/1‑55 (MQ=255)                                                                                               tAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTggg                                                                           >  2:776669/1‑79 (MQ=255)                                                                                               tAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTggg                                                                           <  1:776669/79‑1 (MQ=255)                                                                                               tAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTc               <  1:482193/139‑1 (MQ=255)                                                                                                  ccTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGAc            <  1:1642956/139‑1 (MQ=255)                                                                                                       cTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAg                       <  2:820931/123‑1 (MQ=255)                                                                                                       cTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAg                       >  1:820931/1‑123 (MQ=255)                                                                                                       cTCACCGAGCTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCCTTTGCCAGGTCGAGCAGTTGTCGACTGgag       <  1:1103200/139‑1 (MQ=255)                                                                                                           ccGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCg                                                             >  2:568023/1‑81 (MQ=255)                                                                                                           ccGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCg                                                             <  1:568023/81‑1 (MQ=255)                                                                                                           ccGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGTGATGGTGATGTCTTTTTCCa                              <  1:1149414/112‑1 (MQ=255)                                                                                                           ccGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCa                              >  2:1149414/1‑112 (MQ=255)                                                                                                           ccGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtg   >  1:567484/1‑139 (MQ=255)                                                                                                            cGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAActgct                                                                              >  1:818849/1‑63 (MQ=255)                                                                                                            cGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAActgct                                                                              <  2:818849/63‑1 (MQ=255)                                                                                                            cGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtga  <  2:622657/139‑1 (MQ=255)                                                                                                            cGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtga  >  1:266790/1‑139 (MQ=255)                                                                                                            cGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtga  <  1:233675/139‑1 (MQ=255)                                                                                                                ttGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcg                                                                                              <  2:393603/43‑1 (MQ=255)                                                                                                                ttGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcg                                                                                              >  1:393603/1‑43 (MQ=255)                                                                                                                ttGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGa                                          <  1:610837/95‑1 (MQ=255)                                                                                                                ttGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGa                                          >  2:610837/1‑95 (MQ=255)                                                                                                                        gAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgt                                                                          <  1:25959/55‑1 (MQ=255)                                                                                                                        gAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgt                                                                          >  2:25959/1‑55 (MQ=255)                                                                                                                                  cgcgCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgt                                                                          <  2:972654/45‑1 (MQ=255)                                                                                                                                  cgcgCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgt                                                                          >  1:972654/1‑45 (MQ=255)                                                                                                                                     |                                                                                                                  CGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGA  >  NC_000913/1892270‑1892515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑