Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,905,805 G→A G40S (GGC→AGC)  mntP → putative Mn(2+) efflux pump, mntR‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,905,8050GA97.7% 272.6 / ‑2.3 88G40S (GGC→AGC) mntPputative Mn(2+) efflux pump, mntR‑regulated
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (41/45);  total (42/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

CCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATG  >  NC_000913/1905669‑1905939
                                                                                                                                        |                                                                                                                                      
ccccGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGc                                                                                                                                      >  2:957012/1‑139 (MQ=255)
 cccGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGcc                                                                                                                                     <  1:1559814/139‑1 (MQ=255)
      tCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTAtt                                                                                                                                >  2:323868/1‑139 (MQ=255)
      tCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTAttt                                                                                                                               >  2:57274/1‑139 (MQ=255)
             aTTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTg                                                                                                                         <  1:957012/139‑1 (MQ=255)
             aTTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTg                                                                                                                         <  1:1409401/139‑1 (MQ=255)
                           cACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGt                                                                                                                          <  1:728963/124‑1 (MQ=255)
                           cACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGt                                                                                                                          >  2:728963/1‑124 (MQ=255)
                               gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgc                                                                                                       >  2:255116/1‑139 (MQ=255)
                                cTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGcc                                                                                                                                     >  2:237099/1‑108 (MQ=255)
                                cTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGcc                                                                                                                                     <  1:237099/108‑1 (MQ=255)
                                cTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGt                                                                                                                          <  1:1207241/119‑1 (MQ=255)
                                cTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGt                                                                                                                          >  2:1207241/1‑119 (MQ=255)
                                cTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgcc                                                                                                      >  2:1247363/1‑139 (MQ=255)
                                     gTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                <  1:57274/139‑1 (MQ=255)
                                      ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                <  1:1615557/139‑1 (MQ=255)
                                      ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                <  2:802397/139‑1 (MQ=255)
                                           cttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGa                                                                                                                  >  1:14163/1‑116 (MQ=255)
                                           cttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTGCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGa                                                                                                                  <  2:14163/116‑1 (MQ=255)
                                                 tttGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTg                                                                                                                         >  1:1334427/1‑103 (MQ=255)
                                                 tttGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTg                                                                                                                         <  2:1334427/103‑1 (MQ=255)
                                                         gTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGa                                                                                                          <  1:229806/110‑1 (MQ=255)
                                                         gTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACACTCCATAACCCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGa                                                                                                          >  2:229806/1‑110 (MQ=255)
                                                             atggatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGaa                                                                                     <  1:1331412/126‑1 (MQ=255)
                                                             atggatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGaa                                                                                     >  2:1331412/1‑126 (MQ=255)
                                                                gatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGa                                                                                      >  1:710492/1‑123 (MQ=255)
                                                                gatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGa                                                                                      <  2:710492/123‑1 (MQ=255)
                                                                gatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                >  1:262997/1‑113 (MQ=255)
                                                                gatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                <  2:262997/113‑1 (MQ=255)
                                                                gatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagcca                                                                      >  2:1520093/1‑139 (MQ=255)
                                                                  tgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagccagc                                                                    >  1:1374036/1‑139 (MQ=255)
                                                                    cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTg                                                                                                                         >  2:1174581/1‑84 (MQ=255)
                                                                    cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTg                                                                                                                         <  1:1174581/84‑1 (MQ=255)
                                                                    cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTg                                                                                                  <  1:537454/107‑1 (MQ=255)
                                                                    cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTg                                                                                                  >  2:537454/1‑107 (MQ=255)
                                                                    cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCg                                                                  >  2:1201195/1‑139 (MQ=255)
                                                                    cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                >  2:1114339/1‑108 (MQ=255)
                                                                    cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                <  1:1114339/108‑1 (MQ=255)
                                                                    cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATAGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGa                                                                                                          <  2:781578/99‑1 (MQ=255)
                                                                    cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATAGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGa                                                                                                          >  1:781578/1‑99 (MQ=255)
                                                                             cATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATc                                                                                               >  1:1434283/1‑101 (MQ=255)
                                                                             cATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATc                                                                                               <  2:1434283/101‑1 (MQ=255)
                                                                             cATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCt                                                         <  2:1619464/139‑1 (MQ=255)
                                                                                  aTCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAAt                                                    >  2:1201036/1‑139 (MQ=255)
                                                                                    cGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATgg                                                  <  1:1201195/139‑1 (MQ=255)
                                                                                     ggTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                <  2:325674/92‑1 (MQ=255)
                                                                                     ggTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                >  1:325674/1‑92 (MQ=255)
                                                                                       tAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATgg                                                                                  <  1:175836/104‑1 (MQ=255)
                                                                                       tAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATgg                                                                                  >  2:175836/1‑104 (MQ=255)
                                                                                            gttccACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATATTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                >  2:980182/4‑85 (MQ=255)
                                                                                            gTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                <  1:980182/85‑1 (MQ=255)
                                                                                               ccACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                <  1:924234/82‑1 (MQ=255)
                                                                                               ccACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                >  2:924234/1‑82 (MQ=255)
                                                                                               ccACCCTCCATAAACCGAAATTTTCTGAAGCATGGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGAt                                       <  1:1247363/139‑1 (MQ=255)
                                                                                                 aCCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGa                                                                                      >  2:1422022/1‑90 (MQ=255)
                                                                                                 aCCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGa                                                                                      <  1:1422022/90‑1 (MQ=255)
                                                                                                 aCCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTg                                     >  2:607615/1‑139 (MQ=255)
                                                                                                      ccATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGt                                                                <  1:620385/107‑1 (MQ=255)
                                                                                                      ccATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGt                                                                >  2:620385/1‑107 (MQ=255)
                                                                                                       cATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATgg                                                                                  <  2:677303/88‑1 (MQ=255)
                                                                                                       cATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATgg                                                                                  >  1:677303/1‑88 (MQ=255)
                                                                                                            aCCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATgg                                                                                  <  2:907931/83‑1 (MQ=255)
                                                                                                            aCCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATgg                                                                                  >  1:907931/1‑83 (MQ=255)
                                                                                                             ccGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGtgctgc                         <  1:607615/139‑1 (MQ=255)
                                                                                                                aaaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                >  2:202488/1‑65 (MQ=255)
                                                                                                                aaaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                <  1:8051/65‑1 (MQ=255)
                                                                                                                aaaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                >  2:8051/1‑65 (MQ=255)
                                                                                                                aaaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                <  1:202488/65‑1 (MQ=255)
                                                                                                                aaaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtgtg                             <  2:1480587/132‑1 (MQ=255)
                                                                                                                aaaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtgtg                             >  1:1480587/1‑132 (MQ=255)
                                                                                                                 aaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATgg                                                                                  >  1:1419543/1‑78 (MQ=255)
                                                                                                                 aaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATgg                                                                                  <  2:1419543/78‑1 (MQ=255)
                                                                                                                 aaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                <  1:65629/63‑1 (MQ=255)
                                                                                                                 aaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                >  2:65629/1‑63 (MQ=255)
                                                                                                                       cTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgc                                                                                                       <  1:868655/51‑1 (MQ=255)
                                                                                                                       cTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgc                                                                                                       >  2:868655/1‑51 (MQ=255)
                                                                                                                           aGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTcggc           <  1:323868/139‑1 (MQ=255)
                                                                                                                            gCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTcggcg          <  1:1520093/139‑1 (MQ=255)
                                                                                                                              aTTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCggcggg        <  1:1201036/139‑1 (MQ=255)
                                                                                                                               ttGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCggcgggc       >  2:636285/1‑139 (MQ=255)
                                                                                                                                tGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGt                                                                <  2:229381/81‑1 (MQ=255)
                                                                                                                                tGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGt                                                                >  1:229381/1‑81 (MQ=255)
                                                                                                                                tGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGAt                                       >  2:840229/1‑106 (MQ=255)
                                                                                                                                tGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGAt                                       <  1:840229/106‑1 (MQ=255)
                                                                                                                                tGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCggcgggcg      <  2:1374036/139‑1 (MQ=255)
                                                                                                                                  cGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGAt                                       >  1:208316/1‑104 (MQ=255)
                                                                                                                                  cGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGAt                                       <  2:208316/104‑1 (MQ=255)
                                                                                                                                    aaCCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATg  <  1:636285/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATG  >  NC_000913/1905669‑1905939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: