Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 184,257 | C→T | *271* (TAG→TAA) | yaeI ← | phosphodiesterase with model substrate bis‑pNPP |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 184,257 | 0 | C | T | 100.0% | 235.4 / NA | 73 | *271* (TAG→TAA) | yaeI | phosphodiesterase with model substrate bis‑pNPP |
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (38/35); total (38/35) |
TGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTA > NC_000913/184124‑184383 | tgtgCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTacac < 1:1043486/139‑1 (MQ=255) tgtgCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTacac < 1:567688/139‑1 (MQ=255) gtgCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACAcc < 2:1076578/139‑1 (MQ=255) cTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGt > 1:928891/1‑139 (MQ=255) tttAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGt > 1:391677/1‑134 (MQ=255) tttAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGt < 2:391677/134‑1 (MQ=255) tggtgtggccAGCACGGCTCGATTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTCATACAGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTAc < 2:928891/134‑1 (MQ=255) ggcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTacac < 2:23514/120‑1 (MQ=255) ggcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTacac > 1:23514/1‑120 (MQ=255) ggcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGc > 2:78237/1‑130 (MQ=255) ggcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGc < 1:78237/130‑1 (MQ=255) ggcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACt > 2:1380498/1‑139 (MQ=255) ccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAg < 2:1097439/139‑1 (MQ=255) aCTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCGGTTCCAGc > 1:585192/1‑110 (MQ=255) aCTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCGGTTCCAGc < 2:585192/110‑1 (MQ=255) tgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCTGGGCGGCAGTTCAGACGCaaa > 1:993987/1‑139 (MQ=255) tgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaaa > 1:486535/1‑139 (MQ=255) gCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATa > 1:1295993/1‑139 (MQ=255) aGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGc < 2:572902/99‑1 (MQ=255) aGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGc > 1:572902/1‑99 (MQ=255) aGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACa < 2:486535/139‑1 (MQ=255) aGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACa > 1:625676/1‑139 (MQ=255) cTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGcc < 2:993987/139‑1 (MQ=255) tttttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGccc > 1:1070772/1‑139 (MQ=255) cttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcc < 2:1544859/139‑1 (MQ=255) ttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcca < 2:625676/139‑1 (MQ=255) tcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATGCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac < 2:1070772/139‑1 (MQ=255) ccAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTacac < 2:870218/73‑1 (MQ=255) ccAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTacac > 1:870218/1‑73 (MQ=255) aCTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGtt < 1:1380498/139‑1 (MQ=255) ttCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTtgt > 1:1225783/1‑139 (MQ=255) tCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTtgtg < 2:1225783/139‑1 (MQ=255) tCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTtgtg < 2:404985/139‑1 (MQ=255) tCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGa > 2:479943/1‑139 (MQ=255) tGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGAtgt > 2:950943/1‑139 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTacac > 1:126119/1‑57 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTacac < 2:126119/57‑1 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGc < 1:1596414/67‑1 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGc > 2:1596414/1‑67 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAg > 2:531692/1‑88 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAg < 1:531692/88‑1 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGcc > 2:1614995/1‑114 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGcc < 2:783389/114‑1 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGcc > 1:783389/1‑114 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGcc < 1:1614995/114‑1 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccacg < 1:1059486/123‑1 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccacg > 2:1059486/1‑123 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATgtgt > 2:129072/1‑139 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTAGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa < 2:971417/99‑1 (MQ=255) gCGTAAAAATCAAAAAATTACCTGCTTTATTCTAGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa > 1:971417/1‑99 (MQ=255) tAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCtt > 1:541973/1‑139 (MQ=255) aaaaTCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTc < 1:129072/139‑1 (MQ=255) aaaaTCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTc > 2:36192/1‑139 (MQ=255) aaaTCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCg > 2:790710/1‑139 (MQ=255) aaaaaaTTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCaaa < 2:541973/139‑1 (MQ=255) aTTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGtt < 2:189519/62‑1 (MQ=255) aTTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGtt > 1:189519/1‑62 (MQ=255) ttCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGAc > 2:215459/1‑139 (MQ=255) tCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACg < 1:479943/139‑1 (MQ=255) gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACaaa > 2:731781/1‑75 (MQ=255) gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACaaa < 1:731781/75‑1 (MQ=255) gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCaa > 1:1188562/1‑115 (MQ=255) gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCaa < 2:1188562/115‑1 (MQ=255) gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAg < 1:1227228/117‑1 (MQ=255) gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAg > 2:1227228/1‑117 (MQ=255) gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACg > 1:1235264/1‑135 (MQ=255) gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACg < 2:1235264/135‑1 (MQ=255) gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAAc > 2:146165/1‑139 (MQ=255) gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAAc > 2:1293481/1‑139 (MQ=255) aaaaTTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTa > 1:374147/1‑139 (MQ=255) aaaaTTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTa > 2:934574/1‑139 (MQ=255) tCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTa < 2:719327/127‑1 (MQ=255) tCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTa > 1:719327/1‑127 (MQ=255) | TGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTA > NC_000913/184124‑184383 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |