Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,085,831 A→G L411P (CTG→CCG)  plaP ← putrescine importer, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,8310AG100.0% 721.7 / NA 195L411P (CTG→CCG) plaPputrescine importer, low affinity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (100/95);  total (100/95)

Only 100 of 195 total aligned reads displayed.

TGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAG  >  NC_000913/2085701‑2085964
                                                                                                                                  |                                                                                                                                     
tgatTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCa                                                                                                                               >  2:216537/1‑139 (MQ=255)
          aCGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCattg                                                                                                                     <  1:626442/139‑4 (MQ=255)
              cTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcacc                                                                                                                 >  1:83947/1‑139 (MQ=255)
                 cGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcac                                                                                                                  >  1:342587/1‑135 (MQ=255)
                 cGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcaca                                                                                                              >  2:438739/1‑139 (MQ=255)
                  gTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                          >  1:1613154/1‑126 (MQ=255)
                   tACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            <  2:347857/139‑1 (MQ=255)
                       gCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            <  1:621697/135‑1 (MQ=255)
                       gCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            >  2:621697/1‑135 (MQ=255)
                        cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                          >  1:1013851/1‑120 (MQ=255)
                        cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                     >  2:822167/1‑125 (MQ=255)
                        cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcac                                                                                                                  <  1:1410260/128‑1 (MQ=255)
                        cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcac                                                                                                                  >  2:1410260/1‑128 (MQ=255)
                        cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       >  2:359307/1‑139 (MQ=255)
                        cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       >  1:1529079/1‑139 (MQ=255)
                        cGGAACCGGATTACGGAAGCTCCTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACgct                                                                                                                          <  2:1013851/120‑3 (MQ=255)
                          gAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAAc                                                                                                                             <  1:1031204/115‑1 (MQ=255)
                                     cGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACt                                                                                          <  1:1162495/139‑1 (MQ=255)
                                          gCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            <  2:1047316/116‑1 (MQ=255)
                                          gCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  <  2:509123/126‑1 (MQ=255)
                                          gCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGa                                                                                                   >  2:302253/1‑125 (MQ=255)
                                           ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGt                                                                                    >  1:1122072/1‑139 (MQ=255)
                                           ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGt                                                                                    >  1:1575021/1‑139 (MQ=255)
                                              ttGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGAt                                                                                 <  1:216537/139‑1 (MQ=255)
                                                ggTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                          <  2:1226354/96‑1 (MQ=255)
                                                ggTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCt                                                                               >  2:1051422/1‑139 (MQ=255)
                                                     cGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGTTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGCAGTGATCTTTGAg                                                                          >  2:1119088/1‑139 (MQ=255)
                                                     cGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                                >  1:1579776/1‑117 (MQ=255)
                                                      gCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                        <  2:1336231/108‑1 (MQ=255)
                                                      gCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                        >  1:1336231/1‑108 (MQ=255)
                                                      gCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGc                                                                         <  2:1122072/139‑1 (MQ=255)
                                                      gCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGc                                                                         <  2:1575589/139‑1 (MQ=255)
                                                               gTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            <  2:1421885/95‑1 (MQ=255)
                                                               gTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            >  1:1421885/1‑95 (MQ=255)
                                                                tAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTa                                                                >  1:859576/1‑138 (MQ=255)
                                                                 aaaTCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                              <  2:1205768/139‑1 (MQ=255)
                                                                    tCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGa                                                                                       <  1:704691/111‑1 (MQ=255)
                                                                        aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                     >  1:1469339/1‑77 (MQ=255)
                                                                         ccGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            <  1:1205142/85‑1 (MQ=255)
                                                                         ccGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                      >  1:1603324/1‑139 (MQ=255)
                                                                            aTAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGa                                                   >  1:92348/1‑139 (MQ=255)
                                                                                  gcCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                          >  1:1660570/1‑62 (MQ=255)
                                                                                    ccAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCGCGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAAc                                           >  2:1194802/1‑139 (MQ=255)
                                                                                    ccAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAAc                                           >  1:898002/1‑139 (MQ=255)
                                                                                    ccAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAAc                                           >  1:1222949/1‑139 (MQ=255)
                                                                                      aGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            >  2:1323371/1‑72 (MQ=255)
                                                                                      aGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            <  1:1323371/72‑1 (MQ=255)
                                                                                       gATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGc                                        >  1:992725/1‑139 (MQ=255)
                                                                                        aTCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATc                                                                                                          >  2:1588762/1‑72 (MQ=255)
                                                                                        aTCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCg                                       <  2:1379085/139‑1 (MQ=255)
                                                                                        aTCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCg                                       >  2:1571007/1‑139 (MQ=255)
                                                                                           aGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAAc                                                                                                                             >  2:854265/1‑50 (MQ=255)
                                                                                             aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                                <  1:659396/77‑1 (MQ=255)
                                                                                             aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCa                                                                           <  1:1396582/98‑1 (MQ=255)
                                                                                             aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                      <  2:14891/119‑1 (MQ=255)
                                                                                             aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATg                                  >  2:981164/1‑139 (MQ=255)
                                                                                                cAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGAcc                               <  1:981164/139‑1 (MQ=255)
                                                                                                 aGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       <  1:231768/66‑1 (MQ=255)
                                                                                                 aGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCg                              <  2:139672/139‑1 (MQ=255)
                                                                                                  gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAg                                                                                                                       >  1:370475/1‑49 (MQ=255)
                                                                                                  gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       <  1:1235176/65‑1 (MQ=255)
                                                                                                  gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       >  2:1235176/1‑65 (MQ=255)
                                                                                                  gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  >  2:1692270/1‑70 (MQ=255)
                                                                                                  gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  <  1:1692270/70‑1 (MQ=255)
                                                                                                  gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                                >  1:799978/1‑72 (MQ=255)
                                                                                                  gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATa                                                                                            >  2:691757/1‑76 (MQ=255)
                                                                                                  gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                      >  1:1200089/1‑114 (MQ=255)
                                                                                                  gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGa                             >  1:407722/1‑139 (MQ=255)
                                                                                                   aaCCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCATCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                            <  1:1119088/139‑1 (MQ=255)
                                                                                                   aaCCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGAAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                            <  1:155271/139‑1 (MQ=255)
                                                                                                       aTTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            >  2:264274/1‑55 (MQ=255)
                                                                                                       aTTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                        >  1:591664/1‑91 (MQ=255)
                                                                                                        ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCa                                                                                                                        >  2:1264275/1‑42 (MQ=255)
                                                                                                        ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCa                                                                                                                        <  1:1264275/42‑1 (MQ=255)
                                                                                                        ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                        <  1:1047368/90‑1 (MQ=255)
                                                                                                        ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                              <  2:595709/100‑1 (MQ=255)
                                                                                                             cTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATGCTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGa                  >  1:456421/1‑139 (MQ=255)
                                                                                                             cTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAg                          >  2:1468849/1‑131 (MQ=255)
                                                                                                               ttcttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATc                                                                                                          <  2:786098/49‑1 (MQ=255)
                                                                                                                  ttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTg             >  1:1555768/1‑139 (MQ=255)
                                                                                                                   tcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                      <  2:1605681/49‑1 (MQ=255)
                                                                                                                       ggTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGc        <  1:1494123/139‑1 (MQ=255)
                                                                                                                          tAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  <  2:869396/46‑1 (MQ=255)
                                                                                                                          tAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCgaga                                    >  1:436741/1‑108 (MQ=255)
                                                                                                                          tAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAg                          >  2:626818/1‑118 (MQ=255)
                                                                                                                          tAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGccac     <  2:992725/139‑1 (MQ=255)
                                                                                                                            aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       <  2:410276/39‑1 (MQ=37)
                                                                                                                            aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                        <  2:1635770/70‑1 (MQ=255)
                                                                                                                            aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTa                                                                <  2:1150633/78‑1 (MQ=255)
                                                                                                                            aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                      >  2:1203191/1‑88 (MQ=255)
                                                                                                                            aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATc                                                 >  2:1550647/1‑93 (MQ=255)
                                                                                                                            aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAg                          <  1:604054/116‑1 (MQ=255)
                                                                                                                             cccACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       >  1:136020/1‑38 (MQ=255)
                                                                                                                             cccACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGa                                                                                       <  2:291678/54‑1 (MQ=255)
                                                                                                                             cccACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGa                                                                                       >  1:291678/1‑54 (MQ=255)
                                                                                                                             cccACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                              <  2:394339/79‑1 (MQ=255)
                                                                                                                             cccACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                              >  1:394339/1‑79 (MQ=255)
                                                                                                                             cccACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTaa                     <  2:272639/120‑1 (MQ=255)
                                                                                                                             cccACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTaa                     >  1:272639/1‑120 (MQ=255)
                                                                                                                             cccACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAg  <  1:1571007/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                     
TGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAG  >  NC_000913/2085701‑2085964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑