Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,179,330	T→C	S170S (AGT→AGC)	yegT →	nucleoside transporter, low affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,179,330	0	T	C	100.0%	195.8 / NA	55	S170S (AGT→AGC)	yegT	nucleoside transporter, low affinity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/25);  total (30/25)

TTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTT  >  NC_000913/2179209‑2179461                                                                                                                          |                                                                                                                                    ttCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGgtgt                                                                                                                    >  1:1006114/1‑139 (MQ=255)            ggCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCg                                                                                                             <  2:1242770/135‑1 (MQ=255)            ggCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCg                                                                                                             >  1:1242770/1‑135 (MQ=255)            ggCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGtttt                                                                                                         >  2:1156766/1‑139 (MQ=255)            ggCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGtttt                                                                                                         >  1:379230/1‑139 (MQ=255)            ggCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGATTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGtttt                                                                                                         >  1:781712/1‑139 (MQ=255)                 tATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACACCCCGCTGCTGTTTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTg                                                                                                    <  2:422355/139‑1 (MQ=255)                           aTTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                          <  1:1156766/139‑1 (MQ=255)                               ccTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCGGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGt                                                                                                            >  2:1538676/1‑117 (MQ=255)                               ccTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCGGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGt                                                                                                            <  1:1538676/117‑1 (MQ=255)                               ccTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGtttt                                                                                                         >  1:332129/1‑120 (MQ=255)                               ccTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGtttt                                                                                                         <  2:332129/120‑1 (MQ=255)                                                       tGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGatat                                                              >  2:1042062/1‑139 (MQ=255)                                                              aaTACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGa                                                                                               <  1:1599505/99‑1 (MQ=255)                                                              aaTACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGa                                                                                               >  2:1599505/1‑99 (MQ=255)                                                                   tGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                  <  1:771394/139‑1 (MQ=255)                                                                     gggTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGGGTTTGCGTTTTTCCTGCCCGACACGCCACCaaa                                                                                   <  1:568574/104‑1 (MQ=255)                                                                     gggTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGGGTTTGCGTTTTTCCTGCCCGACACGCCACCaaa                                                                                   >  2:568574/1‑104 (MQ=255)                                                                        tATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGc                                              >  1:456617/1‑138 (MQ=255)                                                                        tATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGc                                              <  2:456617/138‑1 (MQ=255)                                                                          tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACg                                                                                           <  1:577136/91‑1 (MQ=255)                                                                          tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACg                                                                                           >  2:577136/1‑91 (MQ=255)                                                                           gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGcc                                             <  1:989802/136‑1 (MQ=255)                                                                           gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGcc                                             >  2:989802/1‑136 (MQ=255)                                                                           gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGc                                              <  1:482584/135‑1 (MQ=255)                                                                           gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGc                                              >  2:482584/1‑135 (MQ=255)                                                                           gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTgg                                          >  1:920201/1‑139 (MQ=255)                                                                           gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTgg                                          >  2:106765/1‑139 (MQ=255)                                                                             cGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGAt                                        <  2:379230/139‑1 (MQ=255)                                                                               atatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                  <  2:1233827/127‑1 (MQ=255)                                                                               atatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                  >  1:1233827/1‑127 (MQ=255)                                                                               atatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgc                                      >  2:916246/1‑139 (MQ=255)                                                                                tatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgcg                                     >  2:1033953/1‑139 (MQ=255)                                                                                tatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgcg                                     <  2:920201/139‑1 (MQ=255)                                                                                         gACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCctg                            >  2:792521/1‑139 (MQ=255)                                                                                         gACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCctg                            <  1:792521/139‑1 (MQ=255)                                                                                           cTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                          <  2:631734/75‑1 (MQ=255)                                                                                           cTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                          >  1:631734/1‑75 (MQ=255)                                                                                            tAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATgg                                                                  >  2:183445/1‑98 (MQ=255)                                                                                            tAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATgg                                                                  <  1:183445/98‑1 (MQ=255)                                                                                              aCATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGaca                                                                                             <  2:805277/69‑1 (MQ=255)                                                                                              aCATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGaca                                                                                             >  1:805277/1‑69 (MQ=255)                                                                                              aCATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                          >  2:309276/1‑72 (MQ=255)                                                                                              aCATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                          <  1:309276/72‑1 (MQ=255)                                                                                              aCATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTgcg                       <  1:106765/139‑1 (MQ=255)                                                                                              aCATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTgcg                       >  1:815704/1‑139 (MQ=255)                                                                                                  cccGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGaca                                                                                             >  1:447484/1‑65 (MQ=255)                                                                                                  cccGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGaca                                                                                             <  2:447484/65‑1 (MQ=255)                                                                                                  cccGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                          >  2:1431710/1‑68 (MQ=255)                                                                                                  cccGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                          <  1:1431710/68‑1 (MQ=255)                                                                                                    cGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCctgctg                         >  1:653318/1‑131 (MQ=255)                                                                                                    cGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCctgctg                         <  2:653318/131‑1 (MQ=255)                                                                                                    cGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTgcg                       >  1:1446357/1‑133 (MQ=255)                                                                                                    cGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTgcg                       <  2:1446357/133‑1 (MQ=255)                                                                                                                   ccgGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCtt  >  2:1562031/1‑139 (MQ=255)                                                                                                                          |                                                                                                                                    TTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTT  >  NC_000913/2179209‑2179461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑