Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,370,019	T→C	*661R (TGA→CGA)  M1T (ATG→ACG) †	arnA → arnD →	fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase undecaprenyl phosphate‑alpha‑L‑ara4FN deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,370,019	0	T	C	100.0%	172.5 / NA	48	*661R (TGA→CGA)  M1T (ATG→ACG) †	arnA arnD	fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase undecaprenyl phosphate‑alpha‑L‑ara4FN deformylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/24);  total (24/24)

GCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCATGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACAATATGGGC  >  NC_000913/2369889‑2370155                                                                                                                                   |                                                                                                                                         gCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAg                                                                                                                                  <  1:1343571/139‑1 (MQ=255)   aTCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTa                                                                                                                                >  1:1178235/1‑139 (MQ=255)     cGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAgg                                                                                                                              <  1:1141292/139‑1 (MQ=255)      gTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGc                                                                                                                             >  2:1061511/1‑139 (MQ=255)         aaCCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTa                                                                                                                          <  2:1178235/139‑1 (MQ=255)              aGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAt                                                                                                                     >  2:870846/1‑139 (MQ=255)                             cACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCtt                                                                                                      >  2:798256/1‑139 (MQ=255)                                   tGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTgg                                                                                                <  1:1493669/139‑1 (MQ=255)                                    gCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                               <  1:1365470/139‑1 (MQ=255)                                    gCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                               <  1:1061511/139‑1 (MQ=255)                                    gCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                               <  2:137158/139‑1 (MQ=255)                                             gggAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGaa                                                                                      >  1:1535012/1‑139 (MQ=255)                                               gAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAgg                                                                                    >  2:1424747/1‑139 (MQ=255)                                                    gAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGc                                                                               <  1:1424747/139‑1 (MQ=255)                                                          tGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTc                                                                         <  2:1535012/139‑1 (MQ=255)                                                                 cAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGc                                                                                                                             <  2:606876/80‑1 (MQ=255)                                                                 cAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGc                                                                                                                             >  1:606876/1‑80 (MQ=255)                                                                         cATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAtt                                                                                                                    >  1:1471715/1‑81 (MQ=255)                                                                         cATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAtt                                                                                                                    <  2:1471715/81‑1 (MQ=255)                                                                           tcaacgaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAg                                                                                                                                  >  2:1645357/4‑65 (MQ=255)                                                                           tcaacgaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAg                                                                                                                                  <  1:1645357/62‑1 (MQ=255)                                                                           tCGACGAAACGCTGGGTTTCTTCCTGCGAACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGc                                                                                                                             <  2:1271678/70‑1 (MQ=255)                                                                           tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGc                                                                                                                             >  1:1271678/1‑70 (MQ=255)                                                                           tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                        >  2:1296486/1‑139 (MQ=255)                                                                           tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                        <  1:870846/139‑1 (MQ=255)                                                                            cgacgaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTg                                                                                                 >  2:884243/1‑97 (MQ=255)                                                                            cgacgaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTg                                                                                                 <  1:884243/97‑1 (MQ=255)                                                                                gaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCa                                                   <  1:798256/139‑1 (MQ=255)                                                                                  aaCGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCata                                                 >  2:1594003/1‑139 (MQ=255)                                                                                       tGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATAtt                                            >  2:442594/1‑139 (MQ=255)                                                                                         gATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                        >  1:1451950/1‑125 (MQ=255)                                                                                         gATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                        <  2:1451950/125‑1 (MQ=255)                                                                                                   tGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGa                                                                  >  1:386632/1‑105 (MQ=255)                                                                                                   tGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGa                                                                  <  2:386632/105‑1 (MQ=255)                                                                                                     cgcACCGTTGATCTTACGGATAAACCATCACGACCAAAg                                                                                                                                  >  1:526339/1‑39 (MQ=38)                                                                                                     cgcACCGTTGATCTTACGGATAAACCATCACGACCAAAg                                                                                                                                  <  2:526339/39‑1 (MQ=38)                                                                                                            ttGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGc                       >  2:709155/1‑139 (MQ=255)                                                                                                                    aCGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGccc               >  2:1359459/1‑139 (MQ=255)                                                                                                                    aCGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGccc               >  2:802336/1‑139 (MQ=255)                                                                                                                    aCGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCCGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGccc               >  2:278971/1‑139 (MQ=255)                                                                                                                      ggATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                               <  1:1636245/57‑1 (MQ=255)                                                                                                                      ggATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                               >  2:1636245/1‑57 (MQ=255)                                                                                                                      ggATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTctgctg                                                                    <  2:795235/84‑1 (MQ=255)                                                                                                                      ggATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTctgctg                                                                    >  1:795235/1‑84 (MQ=255)                                                                                                                       gATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGa            <  1:1668999/139‑1 (MQ=255)                                                                                                                       gATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGa            <  1:1594003/139‑1 (MQ=255)                                                                                                                         tAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCg              >  2:430299/1‑135 (MQ=255)                                                                                                                         tAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCg              <  1:430299/135‑1 (MQ=255)                                                                                                                                 catGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACAATATGGgc  >  1:118090/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                         GCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCATGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACAATATGGGC  >  NC_000913/2369889‑2370155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑