Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,379,348 C→T *432* (TAG→TAA menF ← isochorismate synthase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,379,3480CT100.0% 84.3 / NA 27*432* (TAG→TAAmenFisochorismate synthase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/11);  total (16/11)

CCAGAATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTG  >  NC_000913/2379214‑2379478
                                                                                                                                      |                                                                                                                                  
ccAGAATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCattattatt                                                                                                                                <  1:564047/139‑1 (MQ=255)
                                   cTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTttg                                                                                             >  2:813552/1‑139 (MQ=255)
                                                         gTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCattattatt                                                                                                                                >  2:1669780/1‑82 (MQ=255)
                                                         gTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCattattatt                                                                                                                                <  1:1669780/82‑1 (MQ=255)
                                                          tATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGtaataa                                                                                                                   <  2:605551/94‑1 (MQ=255)
                                                          tATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGtaataa                                                                                                                   >  1:605551/1‑94 (MQ=255)
                                                                   tGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTc                                                             >  2:441401/1‑139 (MQ=255)
                                                                          aaGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCa                                                      <  1:813552/139‑1 (MQ=255)
                                                                                      cTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCattattatt                                                                                                                                >  1:928702/1‑53 (MQ=255)
                                                                                      cTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCattattatt                                                                                                                                <  2:928702/53‑1 (MQ=255)
                                                                                       tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACa                                                <  2:1337346/132‑1 (MQ=255)
                                                                                       tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACa                                                >  1:1337346/1‑132 (MQ=255)
                                                                                                            tGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCa                                                      >  1:1085692/1‑105 (MQ=255)
                                                                                                            tGAATCGGTAATGATGCGACTCATTATTAGTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCa                                                      <  2:1085692/105‑1 (MQ=255)
                                                                                                                tCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGTCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCa                                                      <  1:1176165/101‑1 (MQ=255)
                                                                                                                tCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGTCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCa                                                      >  2:1176165/1‑101 (MQ=255)
                                                                                                                tCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCa                                                      >  2:1333130/1‑101 (MQ=255)
                                                                                                                tCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCa                                                      <  1:1333130/101‑1 (MQ=255)
                                                                                                                   gTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGcc                                                                                                        <  2:477232/48‑1 (MQ=255)
                                                                                                                   gTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGcc                                                                                                        >  1:477232/1‑48 (MQ=255)
                                                                                                                         atgCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGccc                                                                                                       >  2:1410149/1‑43 (MQ=255)
                                                                                                                         atgCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGccc                                                                                                       <  1:1410149/43‑1 (MQ=255)
                                                                                                                         atgCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTc                                                                   >  2:1045733/1‑79 (MQ=255)
                                                                                                                         atgCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTc                                                                   <  1:1045733/79‑1 (MQ=255)
                                                                                                                          tgCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAAtt      >  1:1334230/1‑139 (MQ=255)
                                                                                                                           gCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGa                                                          >  1:75574/1‑86 (MQ=255)
                                                                                                                           gCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGa                                                          <  2:75574/86‑1 (MQ=255)
                                                                                                                            cGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGt                                                              >  2:1252821/1‑81 (MQ=255)
                                                                                                                            cGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGt                                                              <  1:1252821/81‑1 (MQ=255)
                                                                                                                            cGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAAtttt    >  2:1572113/1‑139 (MQ=255)
                                                                                                                              aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTg  >  1:455807/1‑139 (MQ=255)
                                                                                                                              aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTg  >  1:1923/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                  
CCAGAATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTG  >  NC_000913/2379214‑2379478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: