Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,384,227	A→G	V499A (GTT→GCT)	yfbK ←	Von Willebrand factor domain putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,384,227	0	A	G	100.0%	210.9 / NA	59	V499A (GTT→GCT)	yfbK	Von Willebrand factor domain putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/29);  total (30/29)

GTTTGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAACTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTATCAATTGATGCTTTTTGCCCGTT  >  NC_000913/2384096‑2384360                                                                                                                                    |                                                                                                                                      gTTTGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTg                                                                                                                                <  2:867826/139‑1 (MQ=255)  tttGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGa                                                                                                                               <  1:942622/139‑1 (MQ=255)   ttGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGAc                                                                                                                              <  2:967678/139‑1 (MQ=255)          gctgcCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGActttcttt                                                                                                                       >  1:1605508/1‑139 (MQ=255)            tgcCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTc                                                                                                                     >  2:607475/1‑139 (MQ=255)                                   ttCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCagctaa                                                                                                                                   <  1:423030/102‑1 (MQ=255)                                   ttCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCagctaa                                                                                                                                   >  2:423030/1‑102 (MQ=255)                                     cAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCg                                                                                                  <  1:417416/133‑1 (MQ=255)                                     cAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCg                                                                                                  >  2:417416/1‑133 (MQ=255)                                     cAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAAtttt                                                                                            >  2:1521712/1‑139 (MQ=255)                                     cAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAAtttt                                                                                            >  2:42436/1‑139 (MQ=255)                                        aaCCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCagctaa                                                                                                                                   >  1:282184/1‑97 (MQ=255)                                        aaCCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCagctaa                                                                                                                                   <  2:282184/97‑1 (MQ=255)                                         aCCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAAc                                                                                        <  2:475686/139‑1 (MQ=255)                                          ccGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAAcc                                                                                       >  2:981519/1‑139 (MQ=255)                                             cgTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCt                                                                                                                  >  1:1375137/1‑109 (MQ=255)                                             cgTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCt                                                                                                                  <  2:1375137/109‑1 (MQ=255)                                             cgTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAAc                                                                                        <  2:904728/135‑1 (MQ=255)                                             cgTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAAc                                                                                        >  1:904728/1‑135 (MQ=255)                                              gTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGc                                                                                   <  1:1521712/139‑1 (MQ=255)                                                            tatGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAg                                                                                >  2:681810/1‑128 (MQ=255)                                                            tatGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAg                                                                                <  1:681810/128‑1 (MQ=255)                                                                   cTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTc                                                                 <  2:1159331/136‑1 (MQ=255)                                                                   cTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTc                                                                 >  1:1159331/1‑136 (MQ=255)                                                                        gctgcGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGtt                                                                      <  1:1002114/126‑1 (MQ=255)                                                                        gctgcGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGtt                                                                      >  2:1002114/1‑126 (MQ=255)                                                                         ctgcGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGc                                                        >  1:1277232/1‑139 (MQ=255)                                                                         ctgcGCGAAAACGCATATCTTCAGAGGACGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTATCCGATTTCGc                                                        >  1:1094800/1‑139 (MQ=255)                                                                           gcgcGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTc                                                                                                                     <  1:1284623/76‑1 (MQ=255)                                                                           gcgcGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTc                                                                                                                     >  2:1284623/1‑76 (MQ=255)                                                                             gcgAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAAt                                                    >  2:115219/1‑139 (MQ=255)                                                                                aaaaCGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGAc                                                                                                                              <  2:1192526/62‑1 (MQ=255)                                                                                aaaaCGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGAc                                                                                                                              >  1:1192526/1‑62 (MQ=255)                                                                                aaaaCGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTg                                                 >  1:434174/1‑139 (MQ=255)                                                                                          tCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCgggg                                       <  1:115219/139‑1 (MQ=255)                                                                                          tCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCgggg                                       <  1:607475/139‑1 (MQ=255)                                                                                          tCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCgggg                                       <  2:1607505/139‑1 (MQ=255)                                                                                          tCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCgggg                                       <  1:42436/139‑1 (MQ=255)                                                                                             tCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCAt                                    >  1:1425377/1‑139 (MQ=255)                                                                                                gagGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTg                                                                                                                                >  2:1381251/1‑44 (MQ=255)                                                                                                gagGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTg                                                                                                                                <  1:1381251/44‑1 (MQ=255)                                                                                                 agGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATcc                                           >  2:617804/1‑128 (MQ=255)                                                                                                 agGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATcc                                           <  1:617804/128‑1 (MQ=255)                                                                                                  gggCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGt                               <  1:981519/139‑1 (MQ=255)                                                                                                     cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGc                                                                                                   >  2:1660859/1‑68 (MQ=255)                                                                                                     cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGc                                                                                                   <  1:1660859/68‑1 (MQ=255)                                                                                                     cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCg                                                                                                  <  2:750040/69‑1 (MQ=255)                                                                                                     cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCg                                                                                                  >  1:750040/1‑69 (MQ=255)                                                                                                     cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTa                                                      >  2:1660656/1‑113 (MQ=255)                                                                                                     cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTa                                                      <  1:1660656/113‑1 (MQ=255)                                                                                                     cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCgggg                                       <  1:402044/128‑1 (MQ=255)                                                                                                     cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCgggg                                       >  2:402044/1‑128 (MQ=255)                                                                                                     cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCg                                <  2:1231360/135‑1 (MQ=255)                                                                                                     cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCg                                >  1:1231360/1‑135 (MQ=255)                                                                                                     cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAAc                            >  1:189905/1‑139 (MQ=255)                                                                                                     cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAAc                            >  1:788940/1‑139 (MQ=255)                                                                                                                ttgGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTATCAATTGa                 <  2:1605508/139‑1 (MQ=255)                                                                                                                  ggCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTATCAATTGATg               >  2:1453928/1‑139 (MQ=255)                                                                                                                   gCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAAc                                                                                        >  2:99350/1‑65 (MQ=255)                                                                                                                   gCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAAc                                                                                        <  1:99350/65‑1 (MQ=255)                                                                                                                     cccAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTAGTTTTGTCCGATTTCGCTAATTTGTTCTCCGGAGCATAGCGTAACTTATCAATTGATGCtt            <  2:1094800/139‑1 (MQ=255)                                                                                                                               aTTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTATCAATTGATGCTTTTTGCCCGtt  >  2:1361607/1‑139 (MQ=255)                                                                                                                               aTTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTATCAATTGATGCTTTTTGCCCGtt  <  1:1361607/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      GTTTGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAACTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTATCAATTGATGCTTTTTGCCCGTT  >  NC_000913/2384096‑2384360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑