Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,453,265 C→T pseudogene (1737/2646 nt) yfcU ← pseudogene, FimD fimbrial export usher family,putative membrane, Not classified, putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,453,2650CT100.0% 311.1 / NA 94pseudogene (1737/2646 nt)yfcUpseudogene, FimD fimbrial export usher family,putative membrane, Not classified, putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/48);  total (46/48)

AACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCCC  >  NC_000913/2453140‑2453398
                                                                                                                             |                                                                                                                                     
aaCTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTAt                                                                                                                          <  2:964081/139‑1 (MQ=255)
       aGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCg                                                                                                                   >  2:1219134/1‑139 (MQ=255)
        gTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCgg                                                                                                                  <  1:1219134/139‑1 (MQ=255)
            gTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGccc                                                                                                                    >  2:1394093/1‑133 (MQ=255)
            gTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGccc                                                                                                                    <  1:1394093/133‑1 (MQ=255)
             tCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTc                                                                                                             >  2:1231323/1‑139 (MQ=255)
                cgTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATa                                                                                                          >  2:1437484/1‑139 (MQ=255)
                  tCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACt                                                                                                        >  1:831899/1‑139 (MQ=255)
                        acacGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGc                                                                                                  <  2:907199/139‑1 (MQ=255)
                           cGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGt                                                                                               >  2:448739/1‑139 (MQ=255)
                                             tGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCATGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGc                                                                             <  2:89032/139‑1 (MQ=255)
                                              ggCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                            <  2:597281/139‑1 (MQ=255)
                                              ggCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                            <  1:740317/139‑1 (MQ=255)
                                              ggCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                            <  1:1231323/139‑1 (MQ=255)
                                              ggCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                            >  2:1442470/1‑139 (MQ=255)
                                                   ctgTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCTACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCatat                                                                       >  1:231033/1‑139 (MQ=255)
                                                     gtccgtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACAt                                                                                                                                  >  2:1075135/1‑78 (MQ=255)
                                                     gtccgtccCACTGCCATAGTTGCCGTTATACCTAACGGTACCGTTGTCGCCCCACGGCATACTGAGCGAAATTTACAt                                                                                                                                  <  1:1075135/78‑1 (MQ=255)
                                                        cgtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGcc                                                                                                                     >  1:1172171/1‑88 (MQ=255)
                                                        cgtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGcc                                                                                                                     <  2:1172171/88‑1 (MQ=255)
                                                         gtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTAc                                                                                                                                    <  1:1428523/72‑1 (MQ=255)
                                                         gtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTAc                                                                                                                                    >  2:1428523/1‑72 (MQ=255)
                                                             cACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCt                                                                                                                             <  1:450806/75‑1 (MQ=255)
                                                             cACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCt                                                                                                                             >  2:450806/1‑75 (MQ=255)
                                                                tGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGcc                                                          <  1:145357/139‑1 (MQ=255)
                                                                tGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGcc                                                          <  2:339038/139‑1 (MQ=255)
                                                                tGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGcc                                                          >  2:676918/1‑139 (MQ=255)
                                                                    aTAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATACCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGt                                                                                        >  2:1324147/1‑105 (MQ=255)
                                                                    aTAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATACCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGt                                                                                        <  1:1324147/105‑1 (MQ=255)
                                                                     tAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCatat                                                     >  1:101721/1‑139 (MQ=255)
                                                                     tAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCatat                                                     >  1:1028318/1‑139 (MQ=255)
                                                                          gCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTa                                                                                                    <  2:162428/87‑1 (MQ=255)
                                                                          gCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTa                                                                                                    >  1:162428/1‑87 (MQ=255)
                                                                          gCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGaaa                                                <  1:1442470/139‑1 (MQ=255)
                                                                            cGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGa                                                  <  2:1177879/135‑1 (MQ=255)
                                                                            cGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGa                                                  >  1:1177879/1‑135 (MQ=255)
                                                                                ataGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTgg                                          <  1:676918/139‑1 (MQ=255)
                                                                                ataGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTgg                                          <  1:1437484/139‑1 (MQ=255)
                                                                                ataGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTgg                                          <  2:101721/139‑1 (MQ=255)
                                                                                 taGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGcc                                                          >  1:1498538/1‑122 (MQ=255)
                                                                                 taGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGcc                                                          <  2:1498538/122‑1 (MQ=255)
                                                                                  aGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGga                                        >  1:733651/1‑139 (MQ=255)
                                                                                     tAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGagag                                     <  2:733651/139‑1 (MQ=255)
                                                                                     tAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGagag                                     <  2:831899/139‑1 (MQ=255)
                                                                                       aCGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAAt                                               <  1:1264576/127‑1 (MQ=255)
                                                                                       aCGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAAt                                               >  2:1264576/1‑127 (MQ=255)
                                                                                         ggTACTGTTGTCGCCCTACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCatg                                 <  2:231033/139‑1 (MQ=255)
                                                                                              tgttgtCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGa                                                                                  <  1:1360369/85‑1 (MQ=255)
                                                                                              tgttgtCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGa                                                                                  >  2:1360369/1‑85 (MQ=255)
                                                                                               gttgtCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTa                                                                                              <  2:305277/72‑1 (MQ=255)
                                                                                               gttgtCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTa                                                                                              >  1:305277/1‑72 (MQ=255)
                                                                                               gttgtCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGcc                                                                                           >  1:383352/1‑75 (MQ=255)
                                                                                               gttgtCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGcc                                                                                           <  2:383352/75‑1 (MQ=255)
                                                                                                ttgtCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGccc                                                                                                                    <  2:274791/49‑1 (MQ=255)
                                                                                                ttgtCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGccc                                                                                                                    >  1:274791/1‑49 (MQ=255)
                                                                                                ttgtCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGAtgttgt                          >  1:1565763/1‑139 (MQ=255)
                                                                                                   tCGCCCCACGGCATACTGAGCGAAATTTACATGcc                                                                                                                               >  1:565270/1‑35 (MQ=255)
                                                                                                   tCGCCCCACGGCATACTGAGCGAAATTTACATGcc                                                                                                                               <  2:565270/35‑1 (MQ=255)
                                                                                                   tCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                            >  1:1287577/1‑86 (MQ=255)
                                                                                                   tCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                            <  2:1287577/86‑1 (MQ=255)
                                                                                                     gCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGcc                                                                                                                     <  1:1261767/43‑1 (MQ=255)
                                                                                                     gCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGcc                                                                                                                     >  2:1261767/1‑43 (MQ=255)
                                                                                                          aCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCgg                                                                                                                  <  2:1052337/41‑1 (MQ=255)
                                                                                                          aCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCgg                                                                                                                  >  1:1052337/1‑41 (MQ=255)
                                                                                                          aCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCgg                                                                                                                  <  1:510593/41‑1 (MQ=255)
                                                                                                          aCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCgg                                                                                                                  >  2:510593/1‑41 (MQ=255)
                                                                                                          aCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGcc                                                          >  1:1095482/1‑97 (MQ=255)
                                                                                                          aCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCa                                                        >  2:1266928/1‑99 (MQ=255)
                                                                                                          aCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCa                                                        <  1:1266928/99‑1 (MQ=255)
                                                                                                          aCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGa                                                  >  2:1499363/1‑105 (MQ=255)
                                                                                                          aCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGa                                                  <  1:1499363/105‑1 (MQ=255)
                                                                                                          aCGGCATACTGAGCGAAATTTACATGCCCTTATCCACCCGGTTGTCATACTCGTAGCGGTAGGCAGTCTGGGAAACGCTCATATTACGAATGCTGcc                                                          <  2:1095482/97‑1 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGc                                                                                            >  1:95540/1‑52 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGc                                                                                            <  2:95540/52‑1 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                            <  2:83434/68‑1 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                            <  1:1016337/68‑1 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                            >  1:83434/1‑68 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                            >  2:1016337/1‑68 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGcc                                                          <  1:1585540/86‑1 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGcc                                                          >  2:1585540/1‑86 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGaaa                                                >  1:1479429/1‑96 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGaaa                                                <  2:1479429/96‑1 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGAtgt                             <  1:877607/115‑1 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGAtgt                             >  2:877607/1‑115 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGAt     >  2:205558/1‑139 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGAt     >  1:694675/1‑139 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTAAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGctc             >  2:1118681/1‑131 (MQ=255)
                                                                                                                     aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTACCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGctc             <  1:1118681/131‑1 (MQ=255)
                                                                                                                      gCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATc    >  1:806308/1‑139 (MQ=255)
                                                                                                                       cGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                            >  2:766675/1‑66 (MQ=255)
                                                                                                                       cGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                            <  1:766675/66‑1 (MQ=255)
                                                                                                                       cGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTAc                                                                    >  1:388428/1‑74 (MQ=255)
                                                                                                                       cGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTAc                                                                    <  2:388428/74‑1 (MQ=255)
                                                                                                                        gAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATccc  <  2:806308/139‑1 (MQ=255)
                                                                                                                             |                                                                                                                                     
AACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCCC  >  NC_000913/2453140‑2453398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: