Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,525,930 C→T *309* (TAG→TAA yfeR ← transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,525,9300CT100.0% 170.5 / NA 53*309* (TAG→TAAyfeRtranscriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/26);  total (27/26)

CTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAC  >  NC_000913/2525800‑2526046
                                                                                                                                  |                                                                                                                    
cacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc                                                                                                                >  2:1243299‑M2/127‑137 (MQ=255)
cacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc                                                                                                                <  1:1243299‑M2/11‑1 (MQ=255)
            cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACa                                                                                                         <  2:1536640‑M2/18‑1 (MQ=255)
            cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACa                                                                                                         >  1:1536640‑M2/115‑132 (MQ=255)
                 attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCtt                                                                                             >  1:1242036‑M2/110‑139 (MQ=255)
                 attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCtt                                                                                             >  2:1405922‑M2/110‑139 (MQ=255)
                   tcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtata                                                                                                                  <  1:660817‑M2/9‑1 (MQ=255)
                   tcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgactgattactgccggggctcccggcaCACTTtata                                                                                                                  >  2:660817‑M2/108‑116 (MQ=255)
                        atcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                      >  2:1098628‑M2/103‑139 (MQ=255)
                         tcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                     <  1:516738‑M2/38‑1 (MQ=255)
                                 cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc                                                                                                  >  2:988341‑M2/94‑118 (MQ=255)
                                 cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc                                                                                                  <  1:988341‑M2/25‑1 (MQ=255)
                                   gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATa                                                                                                           >  2:45400‑M2/92‑107 (MQ=255)
                                   gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATa                                                                                                           <  1:45400‑M2/16‑1 (MQ=255)
                                     tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                         <  1:1467971‑M2/50‑1 (MQ=255)
                                     tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                         >  2:1467971‑M2/90‑139 (MQ=255)
                                     tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                         <  2:1242036‑M2/50‑1 (MQ=255)
                                              gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTgg                                                                >  2:930769‑M2/81‑139 (MQ=255)
                                                                ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCAt                                              <  1:1098628‑M2/77‑1 (MQ=255)
                                                                 gatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                >  2:982812‑M2/62‑104 (MQ=255)
                                                                 gatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                <  1:982812‑M2/43‑1 (MQ=255)
                                                                           tctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc                                                                                                                >  1:1255677‑M2/52‑62 (MQ=255)
                                                                           tctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc                                                                                                                <  2:1255677‑M2/11‑1 (MQ=255)
                                                                           tctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                    >  1:1044694‑M2/52‑138 (MQ=255)
                                                                           tctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                    <  2:1044694‑M2/87‑1 (MQ=255)
                                                                                  catcaaatttaacttctgattgattactgccggggctcccgtcaCACTTTATATc                                                                                                                <  2:1156597‑M2/11‑1 (MQ=255)
                                                                                  catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc                                                                                                                >  1:1156597‑M2/45‑55 (MQ=255)
                                                                                  catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc                                                                                                          <  1:436325‑M2/17‑1 (MQ=255)
                                                                                  catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc                                                                                                          >  2:436325‑M2/45‑61 (MQ=255)
                                                                                  catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTg                            >  2:1545816‑M2/45‑139 (MQ=255)
                                                                                  catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTg                            >  1:1660957‑M2/45‑139 (MQ=255)
                                                                                    tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                         <  1:766484‑M2/50‑1 (MQ=255)
                                                                                    tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                         >  2:766484‑M2/43‑92 (MQ=255)
                                                                                    tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                          >  2:138323‑M2/43‑139 (MQ=255)
                                                                                     caaatttaacttctgattgattactgccggggctcccggcaCACTTtata                                                                                                                  <  1:167130‑M2/9‑1 (MQ=255)
                                                                                     caaatttaacttctgattgattactgccggggctcccggcaCACTTtata                                                                                                                  >  2:167130‑M2/42‑50 (MQ=255)
                                                                                         tttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTg                     <  1:1545816‑M2/102‑1 (MQ=255)
                                                                                                    attgattactgccggggctcccggcaCTCTTTATATCTGCTACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc          <  1:930769‑M2/113‑1 (MQ=255)
                                                                                                       gattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTg       <  1:138323‑M2/116‑1 (MQ=255)
                                                                                                        attactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgc      >  1:1082679‑M2/23‑139 (MQ=255)
                                                                                                         ttactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATTAAGCTTCAgcgg                                 >  2:1066757‑M2/22‑111 (MQ=255)
                                                                                                         ttactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcgg                                 <  1:1066757‑M2/90‑1 (MQ=255)
                                                                                                            ctgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAc  <  1:1405922‑M2/121‑1 (MQ=255)
                                                                                                               ccggggctcccggcaCACTTTATATCTGATACAACg                                                                                                      >  2:1089793‑M2/16‑36 (MQ=255)
                                                                                                               ccggggctcccggcaCACTTTATATCTGATACAACg                                                                                                      <  1:1089793‑M2/21‑1 (MQ=255)
                                                                                                                  gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                        >  1:960525‑M2/13‑63 (MQ=255)
                                                                                                                  gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                        <  2:960525‑M2/51‑1 (MQ=255)
                                                                                                                   ggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGGGCa   >  1:1100778‑M2/12‑131 (MQ=255)
                                                                                                                   ggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa   <  2:1100778‑M2/120‑1 (MQ=255)
                                                                                                                       cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGc                                                                                        >  2:708561‑M2/8‑42 (MQ=255)
                                                                                                                       cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGc                                                                                        <  1:708561‑M2/35‑1 (MQ=255)
                                                                                                                         cggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                 >  2:608599‑M2/6‑63 (MQ=255)
                                                                                                                         cggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                 <  1:608599‑M2/58‑1 (MQ=255)
                                                                                                                                  |                                                                                                                    
CTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAC  >  NC_000913/2525800‑2526046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: