Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,591,603	G→A	*119* (TAG→TAA)	yffB →	putative ArsC family reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,591,603	0	G	A	100.0%	196.5 / NA	61	*119* (TAG→TAA)	yffB	putative ArsC family reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/30);  total (31/30)

CCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTG  >  NC_000913/2591467‑2591727                                                                                                                                         |                                                                                                                             ccGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATc                                                                                                                            >  1:994170/1‑139 (MQ=255)     tGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATg                                                                                                                        <  1:440562/139‑1 (MQ=255)       cGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTc                                                                                                                      >  1:1265768/1‑139 (MQ=255)                        ttAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTAt                                                                                                          >  2:35760/1‑134 (MQ=255)                        ttAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTAt                                                                                                          <  1:35760/134‑1 (MQ=255)                        ttAATGACTGAAATGCCTGCAATTATCAAACATCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGc                                                                                                     >  2:1532272/1‑139 (MQ=255)                                tGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACacaa                                                                                             >  2:416257/1‑139 (MQ=255)                                  aaaTGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACacaaca                                                                                           >  2:767933/1‑139 (MQ=255)                                        cTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTAt                                                                                     >  2:1321917/1‑139 (MQ=255)                                         tGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGcc                                                                                                                 <  1:1429951/110‑1 (MQ=255)                                         tGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGcc                                                                                                                 >  2:1429951/1‑110 (MQ=255)                                          gCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTc                                                                                   >  2:1174653/1‑139 (MQ=255)                                                aTCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCgccgcc                                                                             <  1:416257/139‑1 (MQ=255)                                                  cAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGccc                                                                                                                <  2:308325/102‑1 (MQ=255)                                                  cAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGccc                                                                                                                >  1:308325/1‑102 (MQ=255)                                                   aaaCGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCtt                                                                          >  2:1669279/1‑139 (MQ=255)                                                       gTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCt                                                                      <  2:994170/139‑1 (MQ=255)                                                       gTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCt                                                                      <  1:1190573/139‑1 (MQ=255)                                                        tCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGc                                                                                                                  <  1:934947/94‑1 (MQ=255)                                                        tCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGc                                                                                                                  >  2:934947/1‑94 (MQ=255)                                                        tCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcg                                                                                  >  1:1214824/1‑126 (MQ=255)                                                        tCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTg                                                                     >  1:490932/1‑139 (MQ=255)                                                        tCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATGCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcg                                                                                  <  2:1214824/126‑1 (MQ=255)                                                                tctGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtga                                                             <  1:1669279/139‑1 (MQ=255)                                                                 ctGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGccc                                                                                                                <  2:145985/87‑1 (MQ=255)                                                                 ctGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGccc                                                                                                                >  1:145985/1‑87 (MQ=255)                                                                        cccGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAg                                                     <  2:1265768/139‑1 (MQ=255)                                                                         ccGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCt                                                                                                    <  2:1200592/91‑1 (MQ=255)                                                                         ccGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCt                                                                                                    >  1:1200592/1‑91 (MQ=255)                                                                              aaGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTg                                                                                                                   <  2:49266/71‑1 (MQ=255)                                                                              aaGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTg                                                                                                                   >  1:49266/1‑71 (MQ=255)                                                                                     tgctgcTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGa                                                                                                       <  2:350888/76‑1 (MQ=255)                                                                                     tgctgcTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGa                                                                                                       >  1:350888/1‑76 (MQ=255)                                                                                     tgctgcTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAg                                             <  2:103531/134‑1 (MQ=255)                                                                                     tgctgcTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAg                                             >  1:103531/1‑134 (MQ=255)                                                                                      gctgctGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTttg                                       <  1:1321917/139‑1 (MQ=255)                                                                                          ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCt                                                                                        <  1:1414618/86‑1 (MQ=255)                                                                                          ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCt                                                                                        >  2:1414618/1‑86 (MQ=255)                                                                                              gTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGttgattga                               <  1:767933/139‑1 (MQ=255)                                                                                               tttCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTg                                                                     >  1:1539036/1‑100 (MQ=255)                                                                                               tttCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTg                                                                     <  2:1539036/100‑1 (MQ=255)                                                                                                    gTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGcc                                               <  1:916038/117‑1 (MQ=255)                                                                                                    gTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGcc                                               >  2:916038/1‑117 (MQ=255)                                                                                                         tCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTccc                                                                       >  2:663488/1‑88 (MQ=255)                                                                                                         tCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTccc                                                                       <  1:663488/88‑1 (MQ=255)                                                                                                             gTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtga                                                             <  2:1090317/94‑1 (MQ=255)                                                                                                             gTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtga                                                             >  1:1090317/1‑94 (MQ=255)                                                                                                             gTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGAt                <  1:1532272/139‑1 (MQ=255)                                                                                                                 tCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGt            >  2:404393/1‑139 (MQ=255)                                                                                                                  cAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTAtt                                                                                    <  2:998240/66‑1 (MQ=255)                                                                                                                  cAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTAtt                                                                                    >  1:998240/1‑66 (MQ=255)                                                                                                                   aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACacaac                                                                                            <  2:713520/57‑1 (MQ=255)                                                                                                                   aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACacaac                                                                                            >  1:713520/1‑57 (MQ=255)                                                                                                                   aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGttt          >  2:322914/1‑139 (MQ=255)                                                                                                                   aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGttt          >  2:1497485/1‑139 (MQ=255)                                                                                                                   aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGttt          <  1:404393/139‑1 (MQ=255)                                                                                                                        tttttCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCgccgcc                                                                             <  2:509911/67‑1 (MQ=255)                                                                                                                        tttttCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCgccgcc                                                                             >  1:509911/1‑67 (MQ=255)                                                                                                                           ttCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcgccg                                                                               >  2:1121959/1‑62 (MQ=255)                                                                                                                           ttCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcgccg                                                                               <  1:1121959/62‑1 (MQ=255)                                                                                                                           ttCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTg  <  1:322914/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                             CCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTG  >  NC_000913/2591467‑2591727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑