| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,599,313 | G→A | R150C (CGT→TGT) | dapA ← | dihydrodipicolinate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,599,313 | 0 | G | A | 100.0% | 144.6 / NA | 45 | R150C (CGT→TGT) | dapA | dihydrodipicolinate synthase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/24); total (21/24) | |||||||||||
AAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCGACGGA > NC_000913/2599180‑2599434 | aaGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCa < 1:1518824/139‑1 (MQ=255) tCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCg > 1:941774/1‑139 (MQ=255) cAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGtt < 1:340523/139‑1 (MQ=255) cAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGtt < 1:1510221/139‑1 (MQ=255) aGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGttt > 2:730515/1‑139 (MQ=255) aGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGttt > 2:1284111/1‑139 (MQ=255) tCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAg < 2:1360111/139‑1 (MQ=255) ccgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGc < 1:1284111/139‑1 (MQ=255) cgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGc > 1:82000/1‑138 (MQ=255) cgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGc < 2:82000/138‑1 (MQ=255) cTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTc > 2:608816/1‑119 (MQ=255) cTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTc < 1:608816/119‑1 (MQ=255) aCAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACg < 1:555118/139‑1 (MQ=255) tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTc < 1:118899/101‑1 (MQ=255) tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTc > 2:118899/1‑101 (MQ=255) tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCACATCGCAGCCAGTACGGGACGGCACATtat > 1:1146061/1‑139 (MQ=255) cTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATtata > 2:629126/1‑139 (MQ=255) ctctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa > 2:535786/1‑117 (MQ=255) ctctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa < 1:535786/117‑1 (MQ=255) ctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCgg < 2:941774/139‑1 (MQ=255) tttGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGc > 2:351012/1‑139 (MQ=255) ttGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACAGCACATTATACAGAATTTGCGGCa < 2:1146061/139‑1 (MQ=255) tGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAg > 1:623040/1‑139 (MQ=255) tGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAg > 1:1049941/1‑139 (MQ=255) tCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTc < 2:1049941/139‑1 (MQ=255) tCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTc < 2:623040/139‑1 (MQ=255) tttACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGc < 2:1406614/94‑1 (MQ=255) tttACACGCGTTAAGTTCCCTGTTGCCTCTATGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGc > 1:1406614/1‑94 (MQ=255) gTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGa > 1:1040944/1‑139 (MQ=255) ttAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGAt > 2:683802/1‑139 (MQ=255) cTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa < 2:1489449/85‑1 (MQ=255) cTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa > 1:1489449/1‑85 (MQ=255) tGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGc < 1:683802/139‑1 (MQ=255) ccTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATgg < 1:891436/126‑1 (MQ=255) ccTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATgg > 2:891436/1‑126 (MQ=255) ctTTGATTCCGATAATATTTTTTACTTTCGCC‑‑ACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGa > 2:371777/1‑118 (MQ=255) ctTTGATTCCGATAATATTTTTTACTTTCGCC‑‑ACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGa < 1:371777/118‑1 (MQ=255) gATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACaaa < 1:351012/139‑1 (MQ=255) gATAATATTTTTTACTTTCGCCAGACAGCCCATCGTTTCCGGGAGCAGATCGCAGCCAGTa > 2:1003678/1‑61 (MQ=255) gATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTa < 1:1003678/61‑1 (MQ=255) ataataTTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATtat < 2:1523675/76‑1 (MQ=255) ataataTTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATtat > 1:1523675/1‑76 (MQ=255) ttttACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacgga < 2:1040944/139‑1 (MQ=255) tCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCa < 1:1076479/37‑1 (MQ=255) tCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCa > 2:1076479/1‑37 (MQ=255) | AAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCGACGGA > NC_000913/2599180‑2599434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |