Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,673,768	G→A	*141* (TAG→TAA)	yphA →	DoxX family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,673,768	0	G	A	100.0%	201.3 / NA	62	*141* (TAG→TAA)	yphA	DoxX family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/31);  total (31/31)

TGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGC  >  NC_000913/2673650‑2673875                                                                                                                       |                                                                                                            tGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATgcggcg                                                                                         >  2:1020114/1‑139 (MQ=255)   aCCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTg                                                                                       <  1:1020114/139‑1 (MQ=255)       gCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACg                                                                                   >  1:50681/1‑139 (MQ=255)       gCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACg                                                                                   <  1:1395186/139‑1 (MQ=255)           tGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGc                                                                                  <  2:961032/136‑1 (MQ=255)           tGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGc                                                                                  >  1:961032/1‑136 (MQ=255)                  gggCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                        >  2:1118466/1‑139 (MQ=255)                          tatGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGa                                                                                                           <  2:613448/96‑1 (MQ=255)                          tatGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGa                                                                                                           >  1:613448/1‑96 (MQ=255)                              aTTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGACGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTg                                                                                       >  2:1505616/1‑112 (MQ=255)                              aTTAATTTCGGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTg                                                                                       <  1:1505616/112‑1 (MQ=255)                                   tttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTAttaaatgccggat                                                                 <  2:255420/129‑13 (MQ=255)                                   tttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTAttaaatgccggat                                                                 >  1:255420/1‑117 (MQ=255)                                     tCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCa                                                     >  1:1163723/1‑139 (MQ=255)                                                    gTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGa                                      <  1:1118466/139‑1 (MQ=255)                                                          cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTTTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAc                                               <  2:311561/124‑1 (MQ=255)                                                          cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTg                                                                                       <  1:849398/84‑1 (MQ=255)                                                          cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTg                                                                                       >  2:849398/1‑84 (MQ=255)                                                          cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGa                                                                                      <  1:480243/85‑1 (MQ=255)                                                          cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGa                                                                                      >  2:480243/1‑85 (MQ=255)                                                          cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                                       >  1:822186/1‑100 (MQ=255)                                                          cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                                       <  2:822186/100‑1 (MQ=255)                                                          cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAc                                               >  1:311561/1‑124 (MQ=255)                                                          cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACAtctc                                >  1:1338242/1‑139 (MQ=255)                                                             gcgcGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATc                                                                            >  1:27572/1‑92 (MQ=255)                                                             gcgcGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATc                                                                            <  2:27572/92‑1 (MQ=255)                                                               gcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACg                                                                                   <  1:756081/83‑1 (MQ=255)                                                               gcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACg                                                                                   >  2:756081/1‑83 (MQ=255)                                                                       gCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGAt                   <  2:1338242/139‑1 (MQ=255)                                                                           ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAAt                                                             >  2:398766/1‑93 (MQ=255)                                                                           ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAAt                                                             <  1:398766/93‑1 (MQ=255)                                                                             ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAtt                                                                     <  1:956515/83‑1 (MQ=255)                                                                             ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAtt                                                                     >  2:956515/1‑83 (MQ=255)                                                                             ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaa                                                                  >  1:729685/1‑86 (MQ=255)                                                                             ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaa                                                                  <  2:729685/86‑1 (MQ=255)                                                                             ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAAt                                                             >  1:1625101/1‑91 (MQ=255)                                                                             ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAAt                                                             <  2:1625101/91‑1 (MQ=255)                                                                             ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                       <  1:1368985/97‑1 (MQ=255)                                                                             ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                       >  2:1368985/1‑97 (MQ=255)                                                                             ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgtt                         <  2:398082/127‑1 (MQ=255)                                                                             ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgtt                         >  1:398082/1‑127 (MQ=255)                                                                             ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCt             <  2:50681/139‑1 (MQ=255)                                                                                   tACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGa                                                                                      <  2:1429695/60‑1 (MQ=255)                                                                                   tACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGa                                                                                      >  1:1429695/1‑60 (MQ=255)                                                                                   tACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                         <  2:1183977/73‑1 (MQ=255)                                                                                   tACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                         >  1:1183977/1‑73 (MQ=255)                                                                                     ccgggccgggGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAg     <  2:1163723/139‑1 (MQ=255)                                                                                        ggccgggGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGa                                                                                      <  2:1161732/55‑1 (MQ=255)                                                                                        ggccgggGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGa                                                                                      >  1:1161732/1‑55 (MQ=255)                                                                                        ggccgggGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                       >  1:743995/1‑86 (MQ=255)                                                                                        ggccgggGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                       <  2:743995/86‑1 (MQ=255)                                                                                        ggccgggGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgcgc  >  2:1277351/1‑139 (MQ=255)                                                                                             ggggCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                         <  2:1526048/63‑1 (MQ=255)                                                                                             ggggCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                         >  1:1526048/1‑63 (MQ=255)                                                                                              gggCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgc    <  1:710188/131‑1 (MQ=255)                                                                                              gggCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgc    >  2:710188/1‑131 (MQ=255)                                                                                              gggCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAg     >  1:1673086/1‑130 (MQ=255)                                                                                              gggCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAg     <  2:1673086/130‑1 (MQ=255)                                                                                                   aTTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAAt                                                             >  2:1260682/1‑69 (MQ=255)                                                                                                   aTTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAAt                                                             <  1:1260682/69‑1 (MQ=255)                                                                                                            gATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGa                                      >  2:633221/1‑83 (MQ=255)                                                                                                            gATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGa                                      <  1:633221/83‑1 (MQ=255)                                                                                                                       |                                                                                                            TGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGC  >  NC_000913/2673650‑2673875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑