Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 253,084 A→G T126A (ACA→GCA)  yafP → GNAT family putative N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913253,0840AG100.0% 195.0 / NA 54T126A (ACA→GCA) yafPGNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/27);  total (27/27)

AATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGACAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTATGTGCGCGTTTTCT  >  NC_000913/252965‑253210
                                                                                                                       |                                                                                                                              
aaTACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCC‑TTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGtt                                                                                                            <  1:1478650/139‑1 (MQ=255)
 aTACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGtt                                                                                                            <  2:1356493/139‑1 (MQ=255)
 aTACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGtt                                                                                                            <  1:454059/139‑1 (MQ=255)
      cccGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATg                                                                                                       >  1:1612347/1‑139 (MQ=255)
       ccgccgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGc                                                                                                      >  1:333172/1‑139 (MQ=255)
          ccgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCgg                                                                                                   >  2:22531/1‑139 (MQ=255)
           cgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCggg                                                                                                  <  1:1467108/139‑1 (MQ=255)
             tGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGtt                                                                                                            <  2:735801/127‑1 (MQ=255)
             tGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGtt                                                                                                            >  1:735801/1‑127 (MQ=255)
                    gCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGtt                                                                                                            <  2:926898/120‑1 (MQ=255)
                    gCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGtt                                                                                                            >  1:926898/1‑120 (MQ=255)
                         cgcTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCg                                                                                             >  1:194310/1‑130 (MQ=255)
                         cgcTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCg                                                                                             <  2:194310/130‑1 (MQ=255)
                               gTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAAtttt                                                                              >  2:1043809/1‑139 (MQ=255)
                                                   cTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCa                                                          <  2:1126377/139‑1 (MQ=255)
                                                   cTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCa                                                          <  2:333172/139‑1 (MQ=255)
                                                    tGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCaa                                                         >  1:1180372/1‑139 (MQ=255)
                                                      aaTCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACa                                                       >  1:348987/1‑139 (MQ=255)
                                                            aaCTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAAt                                                 >  1:1538824/1‑139 (MQ=255)
                                                                    gTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTatat                                                                         >  1:570771/1‑107 (MQ=255)
                                                                    gTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTatat                                                                         <  2:570771/107‑1 (MQ=255)
                                                                       gACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCaa                                      >  2:201603/1‑139 (MQ=255)
                                                                         cGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATg                                    >  2:527903/1‑139 (MQ=255)
                                                                           cAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAagcagca                                                                                                                   >  1:748484/1‑58 (MQ=255)
                                                                           cAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAagcagca                                                                                                                   <  2:748484/58‑1 (MQ=255)
                                                                               cATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCg                                                                                                    <  2:334754/69‑1 (MQ=255)
                                                                               cATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCg                                                                                                    >  1:334754/1‑69 (MQ=255)
                                                                                  aaCCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcg                           <  1:527903/139‑1 (MQ=255)
                                                                                  aaCCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcg                           <  2:1180372/139‑1 (MQ=255)
                                                                                    ccGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcgcc                         >  1:539519/1‑139 (MQ=255)
                                                                                    ccGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcgcc                         <  2:1612347/139‑1 (MQ=255)
                                                                                      gCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTatat                                                                         <  1:506153/89‑1 (MQ=255)
                                                                                      gCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTatat                                                                         >  2:506153/1‑89 (MQ=255)
                                                                                        aaaaCCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAAt                                                                                                        <  2:931654/56‑1 (MQ=255)
                                                                                        aaaaCCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAAt                                                                                                        >  1:931654/1‑56 (MQ=255)
                                                                                        aaaaCCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCg                                                                                             >  1:489453/1‑67 (MQ=255)
                                                                                        aaaaCCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCg                                                                                             <  2:489453/67‑1 (MQ=255)
                                                                                        aaaaCCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcgcccgcc                     <  1:1043809/139‑1 (MQ=255)
                                                                                        aaaaCCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcgcccgcc                     <  1:201603/139‑1 (MQ=255)
                                                                                          aaCCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCt                                           >  1:534817/1‑115 (MQ=255)
                                                                                          aaCCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCt                                           <  2:534817/115‑1 (MQ=255)
                                                                                                   ttGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTATGTgcg          <  1:22531/139‑1 (MQ=255)
                                                                                                      aaCGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTg                                                                                           >  1:887245/1‑55 (MQ=255)
                                                                                                      aaCGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTg                                                                                           <  2:887245/55‑1 (MQ=255)
                                                                                                       aCGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATG‑AAATAACGCCCGCCTGGTATGTGCGCGttt     >  1:1460254/1‑139 (MQ=255)
                                                                                                        cGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCg                                                                                             <  1:433563/51‑1 (MQ=255)
                                                                                                        cGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCg                                                                                             >  2:433563/1‑51 (MQ=255)
                                                                                                           tATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTATGTGCGCGTTTTCt  >  1:406155/1‑139 (MQ=255)
                                                                                                                ttttCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCggg                                                                                                  >  2:805816/1‑38 (MQ=255)
                                                                                                                ttttCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCggg                                                                                                  <  1:805816/38‑1 (MQ=38)
                                                                                                                  ttCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCg                                                                      <  2:583649/64‑1 (MQ=255)
                                                                                                                  ttCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCg                                                                      >  1:583649/1‑64 (MQ=255)
                                                                                                                   tCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTtat                                                                           <  1:404285/58‑1 (MQ=255)
                                                                                                                   tCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTtat                                                                           >  2:404285/1‑58 (MQ=255)
                                                                                                                       |                                                                                                                              
AATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGACAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTATGTGCGCGTTTTCT  >  NC_000913/252965‑253210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑