Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,697,915	C→T	*212* (TAG→TAA)	pgpC ←	phosphatidylglycerophosphatase C, membrane bound

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,697,915	0	C	T	100.0%	192.0 / NA	59	*212* (TAG→TAA)	pgpC	phosphatidylglycerophosphatase C, membrane bound
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/24);  total (35/24)

CTCATCCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGA‑TTTTGCGCTCCA  >  NC_000913/2697783‑2698048                                                                                                                                     |                                                                                                                                       cTCATCCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTcc                                                                                                                                  >  2:76586/1‑139 (MQ=255)     tCCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGtt                                                                                                                              >  1:1525724/1‑139 (MQ=255)                tttCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCa                                                                                                                   >  2:1237190/1‑139 (MQ=255)                tttCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGAC‑AAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCAc                                                                                                                  >  1:330220/1‑139 (MQ=255)                           tCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGt                                                                                                        <  2:800233/139‑1 (MQ=255)                                                   cGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATg                                                                                         <  1:552652/130‑1 (MQ=255)                                                   cGTGGCTAAATTCGAATTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATg                                                                                         >  2:552652/1‑130 (MQ=255)                                                    gTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACt                                                                                                    >  2:271415/1‑118 (MQ=255)                                                    gTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACt                                                                                                    <  1:271415/118‑1 (MQ=255)                                                    gTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGaaa                                                                               >  1:1558377/1‑139 (MQ=255)                                                     tGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGa                                                                                                                        >  2:851961/1‑97 (MQ=255)                                                     tGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGa                                                                                                                        <  1:851961/97‑1 (MQ=255)                                                      ggCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATa                                                                             <  2:1525724/139‑1 (MQ=255)                                                       gCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATaa                                                                            >  1:798237/1‑139 (MQ=255)                                                        cTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTCTGCTTTACTTTATTCCAGTTGCTAGAGTTCACcgcgc                                                                                                             <  2:1134132/105‑1 (MQ=255)                                                        cTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcgc                                                                                                             >  1:1134132/1‑105 (MQ=255)                                                           aaTTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCgggg                                                                                                         <  2:1483097/106‑1 (MQ=255)                                                           aaTTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCgggg                                                                                                         >  1:1483097/1‑106 (MQ=255)                                                           aaTTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAagca                                                                        >  2:722673/1‑139 (MQ=255)                                                                gACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGa                                                                   <  2:1558377/139‑1 (MQ=255)                                                                gACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGa                                                                   <  1:722673/139‑1 (MQ=255)                                                                gACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGa                                                                   <  2:1027086/139‑1 (MQ=255)                                                                  cTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGt                                                                                                        <  2:390317/100‑1 (MQ=255)                                                                  cTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGt                                                                                                        >  1:390317/1‑100 (MQ=255)                                                                      aGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTcc                                                             >  2:1421503/1‑139 (MQ=255)                                                                                aaaaCTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGAtt                                                                 <  1:1676603/125‑1 (MQ=255)                                                                                aaaaCTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGAtt                                                                 >  2:1676603/1‑125 (MQ=255)                                                                                  aaCTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCg                                                 >  2:1502198/1‑139 (MQ=255)                                                                                      ccGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCa                                                                                                                   >  2:1421036/1‑69 (MQ=255)                                                                                      ccGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCa                                                                                                                   <  1:1421036/69‑1 (MQ=255)                                                                                      ccGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCtat                                             >  2:1381448/1‑139 (MQ=255)                                                                                           tataAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCa                                        <  1:76586/139‑1 (MQ=255)                                                                                            ataAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCAc                                       <  1:1502198/139‑1 (MQ=255)                                                                                                   cgcgGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAg                                >  2:60907/1‑139 (MQ=255)                                                                                                      ggcgCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAagca                                                                        <  1:1609625/96‑1 (MQ=255)                                                                                                      ggcgCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAagca                                                                        >  2:1609625/1‑96 (MQ=255)                                                                                                       gcgcATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGc                            >  1:1446854/1‑139 (MQ=255)                                                                                                                    ggACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGa               >  2:484163/1‑139 (MQ=255)                                                                                                                    ggACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGa               >  2:881027/1‑139 (MQ=255)                                                                                                                    ggACGCTATGCTTTACTTTATT‑CAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGAtt                                                                 >  1:480457/1‑88 (MQ=255)                                                                                                                    ggACGCTATGCTTTACTTTATT‑CAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGAtt                                                                 <  2:480457/88‑1 (MQ=255)                                                                                                                     gACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGa                                                          <  2:862980/95‑1 (MQ=255)                                                                                                                     gACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGa                                                          >  1:862980/1‑95 (MQ=255)                                                                                                                      aCGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                              <  1:241748/42‑1 (MQ=255)                                                                                                                      aCGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                              >  2:241748/1‑42 (MQ=255)                                                                                                                      aCGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGAtt                                                                 <  2:465543/87‑1 (MQ=255)                                                                                                                      aCGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGAtt                                                                 >  1:465543/1‑87 (MQ=255)                                                                                                                       cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                              >  2:1526260/1‑41 (MQ=255)                                                                                                                       cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                              <  1:1526260/41‑1 (MQ=255)                                                                                                                       cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCa                                                                                               <  1:1436830/56‑1 (MQ=255)                                                                                                                       cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCa                                                                                               >  2:1436830/1‑56 (MQ=255)                                                                                                                       cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGA‑ttt           >  2:1638316/1‑139 (MQ=255)                                                                                                                         cTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGA‑TTTTg         >  2:1492521/1‑139 (MQ=255)                                                                                                                         cTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGA‑TTTTg         >  2:647207/1‑139 (MQ=255)                                                                                                                         cTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGA‑TTTTg         >  2:67627/1‑139 (MQ=255)                                                                                                                           aTGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGA‑TTTTgcg       <  1:647207/139‑1 (MQ=255)                                                                                                                            tGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGA‑TTTTgcgc      <  2:1348940/139‑1 (MQ=255)                                                                                                                            tGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGA‑TTTTgcgc      >  1:1348940/1‑139 (MQ=255)                                                                                                                                 tACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTTGCGCTCCa  >  1:1315451/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                       CTCATCCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGA‑TTTTGCGCTCCA  >  NC_000913/2697783‑2698048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑