Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,856,453 C→T *118* (TAG→TAA ygbA ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,856,4530CT100.0% 148.7 / NA 46*118* (TAG→TAAygbAuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/21);  total (25/21)

GCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCT  >  NC_000913/2856336‑2856583
                                                                                                                     |                                                                                                                                  
gcgcAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGa                                                                                                               <  2:1267326/139‑1 (MQ=255)
                  aaaCGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGt                                                                                             >  2:303609/1‑139 (MQ=255)
                  aaaCGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGt                                                                                             >  1:1293086/1‑139 (MQ=255)
                                 ctgTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGc                                                                              >  1:1651088/1‑139 (MQ=255)
                                      aCGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGtt                                                                                            >  2:7572/1‑120 (MQ=255)
                                      aCGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGtt                                                                                            <  1:7572/120‑1 (MQ=255)
                                             aaGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgatg                                                                  <  1:1486318/139‑1 (MQ=255)
                                              aGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgatga                                                                 >  1:583404/1‑139 (MQ=255)
                                                   cTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATAtttt                                                                                                      <  2:698319/97‑1 (MQ=255)
                                                   cTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATAtttt                                                                                                      >  1:698319/1‑97 (MQ=255)
                                                    tGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGAc                                                           <  2:44805/139‑1 (MQ=255)
                                                      ggAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc                                                         <  1:1242659/139‑1 (MQ=255)
                                                       gAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCa                                                        <  1:303609/139‑1 (MQ=255)
                                                             aTAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTa                                                  >  2:389938/1‑139 (MQ=255)
                                                                aaTCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgatgagatg                                                             <  1:555296/125‑1 (MQ=255)
                                                                aaTCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgatgagatg                                                             >  2:555296/1‑125 (MQ=255)
                                                                  tCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGa                                                                                 >  1:419224/1‑103 (MQ=255)
                                                                  tCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGa                                                                                 <  2:419224/103‑1 (MQ=255)
                                                                      cATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGAt                                         <  2:583404/139‑1 (MQ=255)
                                                                               aaCTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCcaca                                <  1:415873/139‑1 (MQ=255)
                                                                                     aaaTTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgatg                                                                  <  1:607144/99‑1 (MQ=255)
                                                                                     aaaTTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgatg                                                                  >  2:607144/1‑99 (MQ=255)
                                                                                     aaaTTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc                                                         >  2:1391786/1‑108 (MQ=255)
                                                                                     aaaTTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc                                                         <  1:1391786/108‑1 (MQ=255)
                                                                                           ccGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTcggcc                    <  1:389938/139‑1 (MQ=255)
                                                                                           ccGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTcggcc                    <  2:1293086/139‑1 (MQ=255)
                                                                                                   aTCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCaa            >  2:232418/1‑139 (MQ=255)
                                                                                                        ccTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGTACGCTTGTCATCGATGAGATGACGCAc                                                       <  1:1094264/91‑1 (MQ=255)
                                                                                                        ccTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCAt       >  1:230175/1‑139 (MQ=255)
                                                                                                        ccTGTCCGGCATATTATTCATGAGGTTTGTTCGGACGATATTTTTCCGGCAGTTCTGGCACTGGACGCTTGTCATCGATGAGATGACGCAc                                                       >  2:1094264/1‑91 (MQ=255)
                                                                                                           gTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgat                                                                   <  1:1483007/76‑1 (MQ=255)
                                                                                                           gTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgat                                                                   >  2:1483007/1‑76 (MQ=255)
                                                                                                           gTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCGTTCTCGGCCCGGCCCAACGCATAAt    >  2:1026791/1‑139 (MQ=255)
                                                                                                           gTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAAt    >  2:528257/1‑139 (MQ=255)
                                                                                                             ccGGCATTTTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGTACGCTTGTCATCGTTGGTCTGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCt  >  2:459465/1‑139 (MQ=255)
                                                                                                             ccGGCATATTATTCATGAGGTTTTTTCGGACTATATTTTTCCGGCAGTTCTGGCACCGGACGCCTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACgt                                    <  1:1039183/105‑2 (MQ=255)
                                                                                                             ccGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTc                                                                                           >  2:546398/1‑50 (MQ=255)
                                                                                                             ccGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTc                                                                                           <  1:546398/50‑1 (MQ=255)
                                                                                                             ccGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGc                                    >  2:1039183/1‑105 (MQ=255)
                                                                                                             ccGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCt  >  2:1174122/1‑139 (MQ=255)
                                                                                                             ccGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCt  >  1:275326/1‑139 (MQ=255)
                                                                                                             ccGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCt  >  1:1168384/1‑139 (MQ=255)
                                                                                                              cGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGAt                                              <  2:1681702/94‑1 (MQ=255)
                                                                                                              cGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGAt                                              >  1:1681702/1‑94 (MQ=255)
                                                                                                                 cATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAg                               >  1:978762/1‑106 (MQ=255)
                                                                                                                 cATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAg                               <  2:978762/106‑1 (MQ=255)
                                                                                                                     |                                                                                                                                  
GCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCT  >  NC_000913/2856336‑2856583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: