Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,983,288	C→T	*279* (TAG→TAA)	kduI ←	hexuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,983,288	0	C	T	100.0%	156.3 / NA	49	*279* (TAG→TAA)	kduI	hexuronate isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/25);  total (24/25)

AGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGAT  >  NC_000913/2983156‑2983413                                                                                                                                     |                                                                                                                              aGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAgcgc                                                                                                                         <  1:737445/139‑1 (MQ=255)                   ccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                       <  2:348394/139‑1 (MQ=255)                            tCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCata                                                                                              <  1:1387010/139‑1 (MQ=255)                                        gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                   <  1:1442767/138‑1 (MQ=255)                                        gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                   >  2:1442767/1‑138 (MQ=255)                                              tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCa                                                                                                                        >  2:447563/1‑95 (MQ=255)                                              tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCa                                                                                                                        <  1:447563/95‑1 (MQ=255)                                               aTCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGtttt                                                                           >  2:649865/1‑139 (MQ=255)                                                   caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCAcc                                                                       >  1:1060554/1‑139 (MQ=255)                                                        aGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCa                                                                  <  1:649865/139‑1 (MQ=255)                                                            acgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                              <  2:1550494/139‑1 (MQ=255)                                                                 cGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                       >  2:391926/1‑93 (MQ=255)                                                                 cGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                       <  1:391926/93‑1 (MQ=255)                                                                   cAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                       <  2:1060554/139‑1 (MQ=255)                                                                         tACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGta                                                 <  2:414/139‑1 (MQ=255)                                                                              tCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGc                                            >  1:916621/1‑139 (MQ=255)                                                                                               tAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCttt                                                                                                                 <  2:1343069/53‑1 (MQ=255)                                                                                               tAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCttt                                                                                                                 >  1:1343069/1‑53 (MQ=255)                                                                                                aaaaTCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa                          >  2:108891/1‑139 (MQ=255)                                                                                                aaaaTCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa                          <  2:685462/139‑1 (MQ=255)                                                                                                aaaaTCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa                          >  1:946418/1‑139 (MQ=255)                                                                                                aaaaTCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa                          <  2:916621/139‑1 (MQ=255)                                                                                                        aaCAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                       >  2:965763/1‑54 (MQ=255)                                                                                                        aaCAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                       <  1:965763/54‑1 (MQ=255)                                                                                                        aaCAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                               >  2:1668889/1‑126 (MQ=255)                                                                                                        aaCAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                               <  1:1668889/126‑1 (MQ=255)                                                                                                        aaCAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCg                  >  1:719689/1‑139 (MQ=255)                                                                                                             tACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                   <  1:457044/69‑1 (MQ=255)                                                                                                             tACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                   >  2:457044/1‑69 (MQ=255)                                                                                                              aCCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGc            <  2:946418/139‑1 (MQ=255)                                                                                                                cTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTc          >  1:1644504/1‑139 (MQ=255)                                                                                                                  tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                       <  1:403671/44‑1 (MQ=255)                                                                                                                  tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                       >  2:403671/1‑44 (MQ=255)                                                                                                                    ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACgg                                                                                                            <  1:541416/37‑1 (MQ=255)                                                                                                                    ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACgg                                                                                                            >  2:541416/1‑37 (MQ=255)                                                                                                                    ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                              >  1:432759/1‑83 (MQ=255)                                                                                                                    ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                              <  2:432759/83‑1 (MQ=255)                                                                                                                    ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                 <  2:1012121/128‑1 (MQ=255)                                                                                                                    ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                 >  1:1012121/1‑128 (MQ=255)                                                                                                                    ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg      >  1:1273777/1‑139 (MQ=255)                                                                                                                     tCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                       >  2:1042803/1‑41 (MQ=255)                                                                                                                     tCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                       <  1:1042803/41‑1 (MQ=255)                                                                                                                        tttATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGAt  >  2:1303997/1‑139 (MQ=255)                                                                                                                         ttATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCa                                                                                                >  2:952142/1‑44 (MQ=255)                                                                                                                         ttATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCa                                                                         >  2:164405/1‑67 (MQ=255)                                                                                                                         ttATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCa                                                                         <  1:164405/67‑1 (MQ=255)                                                                                                                         ttATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                               <  2:900841/109‑1 (MQ=255)                                                                                                                         ttATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGACAAAGGTATAAGCTTTGGTTCCGaca                               >  1:900841/1‑109 (MQ=255)                                                                                                                         ttATGCCCACAATTAGCGCAAATCTTTAACGGCCACAGGGTCCa                                                                                                <  1:952142/44‑1 (MQ=255)                                                                                                                                     |                                                                                                                              AGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGAT  >  NC_000913/2983156‑2983413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑