Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,993,856 G→A *73* (TAG→TAA ygeI → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,993,8560GA100.0% 84.9 / NA 27*73* (TAG→TAAygeIuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/13);  total (14/13)

CATCAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATAT  >  NC_000913/2993727‑2993987
                                                                                                                                 |                                                                                                                                   
caTCAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGAc                                                                                                                            <  2:1517888/139‑1 (MQ=255)
                aCTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTAACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAAca                                                                                                            >  2:727314/1‑138 (MQ=255)
                    aTACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGAt                                                                                                        <  1:727314/139‑1 (MQ=255)
                          aTCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACtttt                                                                                                  >  1:306644/1‑139 (MQ=255)
                           tCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGa                                                                                                                             >  2:642893/1‑111 (MQ=255)
                           tCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGa                                                                                                                             <  1:642893/111‑1 (MQ=255)
                                tACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTataata                                                                                            <  2:306644/139‑1 (MQ=255)
                                  cAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGt                                                                                          <  2:1123317/139‑1 (MQ=255)
                                                    aCATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGa                                                                                                                             >  2:1569067/1‑86 (MQ=255)
                                                    aCATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGa                                                                                                                             <  1:1569067/86‑1 (MQ=255)
                                                         gatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCg                                                                                    >  1:1439891/1‑122 (MQ=255)
                                                         gatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCg                                                                                    <  2:1439891/122‑1 (MQ=255)
                                                                    ttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcattc                                                                        <  2:692781/123‑1 (MQ=255)
                                                                    ttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcattc                                                                        >  1:692781/1‑123 (MQ=255)
                                                                                      cAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCaa                                      >  1:674143/1‑139 (MQ=255)
                                                                                       agaagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGATCACCaaa                                     >  1:1003592/1‑139 (MQ=255)
                                                                                         aagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAg                                   >  1:1382639/1‑139 (MQ=255)
                                                                                                tCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTGTCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAAtt                            >  1:1044516/1‑139 (MQ=255)
                                                                                                tCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAAtt                            >  1:982916/1‑139 (MQ=255)
                                                                                                          ccTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATaa                                                                                                              <  2:544823/47‑1 (MQ=255)
                                                                                                          ccTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATaa                                                                                                              >  1:544823/1‑47 (MQ=255)
                                                                                                          ccTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATAAAAAAATATGCGCTCACCAAAAGTTa                                <  1:622/125‑1 (MQ=255)
                                                                                                          ccTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATAAAAAAATATGCGCTCACCAAAAGTTa                                >  2:622/1‑125 (MQ=255)
                                                                                                            ttACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCtt                <  2:674143/139‑1 (MQ=255)
                                                                                                              aCTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAATAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCa              <  2:1044516/139‑1 (MQ=255)
                                                                                                              aCTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCa              <  2:982916/139‑1 (MQ=255)
                                                                                                                          aGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTatat  >  2:256150/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                   
CATCAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATAT  >  NC_000913/2993727‑2993987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: