Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,078,852 T→C N3S (AAC→AGC)  cmtA ← putative mannitol‑specific PTS IIB and IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,078,8520TC100.0% 147.3 / NA 42N3S (AAC→AGC) cmtAputative mannitol‑specific PTS IIB and IIC components
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/21);  total (21/21)

TCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTT  >  NC_000913/3078720‑3078977
                                                                                                                                    |                                                                                                                             
tCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGTATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCa                                                                                                                         <  2:1235352/139‑1 (MQ=255)
          gCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATc                                                                                                               <  1:1585781/139‑1 (MQ=255)
                                                                  atatTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCtg                                                       >  1:602899/1‑139 (MQ=255)
                                                                      tGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGa                                                                               >  1:1419403/1‑111 (MQ=255)
                                                                      tGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGa                                                                               <  2:1419403/111‑1 (MQ=255)
                                                                          gATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCa                                                                                                                         >  2:337847/1‑65 (MQ=255)
                                                                          gATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCa                                                                                                                         <  1:337847/65‑1 (MQ=255)
                                                                             gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCa                                                                                                                         <  2:1669664/62‑1 (MQ=255)
                                                                             gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCa                                                                                                                         >  1:1669664/1‑62 (MQ=255)
                                                                             gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTa                                                                                                          >  2:184897/1‑77 (MQ=255)
                                                                             gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTa                                                                                                          <  1:184897/77‑1 (MQ=255)
                                                                             gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcg                                                                                      <  2:64178/97‑1 (MQ=255)
                                                                             gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcg                                                                                      >  1:64178/1‑97 (MQ=255)
                                                                             gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                 <  1:1354133/134‑1 (MQ=255)
                                                                             gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                 >  2:1541950/1‑134 (MQ=255)
                                                                             gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                 >  2:1354133/1‑134 (MQ=255)
                                                                             gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                 <  1:1541950/134‑1 (MQ=255)
                                                                              aCCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTa                                                                                                          >  1:674680/1‑76 (MQ=255)
                                                                              aCCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTa                                                                                                          <  2:674680/76‑1 (MQ=255)
                                                                              aCCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGgaga                                                                                                   <  1:1449657/83‑1 (MQ=255)
                                                                              aCCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGgaga                                                                                                   >  2:1449657/1‑83 (MQ=255)
                                                                              aCCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTa                                           >  2:170027/1‑139 (MQ=255)
                                                                                cATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAag                                         >  1:1560171/1‑139 (MQ=255)
                                                                                   tGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTc                                                                        >  2:661177/1‑105 (MQ=255)
                                                                                   tGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTc                                                                        <  1:661177/105‑1 (MQ=255)
                                                                                        tCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCt                                                                    >  2:69722/1‑104 (MQ=255)
                                                                                        tCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCt                                                                    <  1:69722/104‑1 (MQ=255)
                                                                                                  cccAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCataata                                                                                                                    >  1:265833/1‑46 (MQ=255)
                                                                                                  cccAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCataata                                                                                                                    <  2:265833/46‑1 (MQ=255)
                                                                                                  cccAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATttct                       >  2:844725/1‑139 (MQ=255)
                                                                                                     aaaaGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATttcttct                    <  1:170027/139‑1 (MQ=255)
                                                                                                             ggACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATc                                                                                                               >  1:1326800/1‑40 (MQ=255)
                                                                                                             ggACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATc                                                                                                               <  2:1326800/40‑1 (MQ=255)
                                                                                                              gACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGgaga                                                                                                   <  2:243100/51‑1 (MQ=255)
                                                                                                              gACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGgaga                                                                                                   >  1:243100/1‑51 (MQ=255)
                                                                                                              gACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCgg                                                                                   >  2:561644/1‑67 (MQ=255)
                                                                                                              gACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCgg                                                                                   <  1:561644/67‑1 (MQ=255)
                                                                                                                   ttGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTagag                                                                                                       <  1:867375/42‑1 (MQ=255)
                                                                                                                   ttGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTagag                                                                                                       >  2:867375/1‑42 (MQ=255)
                                                                                                                       aCGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACtt  <  2:1560171/139‑1 (MQ=255)
                                                                                                                           gCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcgc                                                                                     >  1:946597/1‑52 (MQ=255)
                                                                                                                           gCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcgc                                                                                     <  2:946597/52‑1 (MQ=255)
                                                                                                                                    |                                                                                                                             
TCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTT  >  NC_000913/3078720‑3078977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: