Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,140,294	T→C	S9G (AGC→GGC)	hybF ←	protein involved with the maturation of hydrogenases 1 and 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,140,294	0	T	C	100.0%	165.8 / NA	46	S9G (AGC→GGC)	hybF	protein involved with the maturation of hydrogenases 1 and 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/24);  total (22/24)

CGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTT  >  NC_000913/3140159‑3140429                                                                                                                                        |                                                                                                                                        cGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTg                                                                                                                                      >  2:1209819/1‑139 (MQ=255)   tGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGc                                                                                                                                    <  2:38838/139‑1 (MQ=255)          cAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAgaga                                                                                                                             <  2:1287156/139‑1 (MQ=255)          cAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAgaga                                                                                                                             <  1:1296527/139‑1 (MQ=255)                            ggacggCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGc                                                                                                           <  2:852442/139‑1 (MQ=255)                                     tctcCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGc                                                                                                  <  1:1209819/139‑1 (MQ=255)                                       tcctcAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGTCGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGa                                                                                                <  1:4604/139‑1 (MQ=255)                                              cGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAAca                                                                                         >  1:837833/1‑139 (MQ=255)                                                 aGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAAcagca                                                                                      >  1:989000/1‑139 (MQ=255)                                                   gagaGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAgg                                                                                    >  1:125369/1‑139 (MQ=255)                                                      agCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGc                                                                                                                                    <  2:544733/88‑1 (MQ=255)                                                      agCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGc                                                                                                                                    >  1:544733/1‑88 (MQ=255)                                                        cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGcgcg                                                                               >  2:220595/1‑139 (MQ=255)                                                                tttCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                                            >  1:1148450/1‑86 (MQ=255)                                                                tttCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                                            <  2:1148450/86‑1 (MQ=255)                                                                  tCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCg                                                                     <  2:837833/139‑1 (MQ=255)                                                                     aGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCgtgt                                                                  >  1:1159975/1‑139 (MQ=255)                                                                          cacGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGc                                                                                                  >  1:1306202/1‑102 (MQ=255)                                                                          cacGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGc                                                                                                  <  2:1306202/102‑1 (MQ=255)                                                                                  tGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGAt                                                     >  2:70290/1‑139 (MQ=255)                                                                                     cgcgCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCgt                                                                    >  1:881278/1‑121 (MQ=255)                                                                                     cgcgCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCgt                                                                    <  2:881278/121‑1 (MQ=255)                                                                                         cTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                                            <  2:905986/61‑1 (MQ=255)                                                                                         cTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                                            >  1:905986/1‑61 (MQ=255)                                                                                         cTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGcc                                                                          <  1:161951/111‑1 (MQ=255)                                                                                         cTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGcc                                                                          >  2:161951/1‑111 (MQ=255)                                                                                         cTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGAc                                              >  1:4980/1‑139 (MQ=255)                                                                                           tAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAgaga                                                                                                                             <  1:1425270/58‑1 (MQ=255)                                                                                           tAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAgaga                                                                                                                             >  2:1425270/1‑58 (MQ=255)                                                                                            aaCATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTgg                                           <  1:182176/139‑1 (MQ=255)                                                                                                  gTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGccct                                                                                                                                       >  2:47381/1‑41 (MQ=255)                                                                                                  gTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGccct                                                                                                                                       <  1:47381/41‑1 (MQ=255)                                                                                                    gctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGc                                                                                                  >  1:1684826/1‑76 (MQ=255)                                                                                                    gctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGc                                                                                                  <  2:1684826/76‑1 (MQ=255)                                                                                                        ctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGa                                                                                                <  1:754622/74‑1 (MQ=255)                                                                                                        ctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGa                                                                                                >  2:754622/1‑74 (MQ=255)                                                                                                         tCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACGACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGcc                                                                          >  2:1685703/1‑95 (MQ=255)                                                                                                         tCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACGACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGcc                                                                          <  1:1685703/95‑1 (MQ=255)                                                                                                           cGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCaa                                                                                                                                  >  1:674965/1‑37 (MQ=255)                                                                                                           cGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCaa                                                                                                                                  <  2:674965/37‑1 (MQ=255)                                                                                                               tGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGt                        <  2:1159975/139‑1 (MQ=255)                                                                                                                  cGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcg                     <  2:125369/139‑1 (MQ=255)                                                                                                                               cAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCa        <  1:70290/139‑1 (MQ=255)                                                                                                                                 aCGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCaaa                                                                                           >  2:1054181/1‑54 (MQ=255)                                                                                                                                 aCGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCaaa                                                                                           <  1:1054181/54‑1 (MQ=255)                                                                                                                                     cgcCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTgg                                           <  2:1029216/98‑1 (MQ=255)                                                                                                                                     cgcCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGtt  >  1:1155793/1‑139 (MQ=255)                                                                                                                                     cgcCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCGGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTgg                                           >  1:1029216/1‑98 (MQ=255)                                                                                                                                        |                                                                                                                                        CGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTT  >  NC_000913/3140159‑3140429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑