| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,158,370 | G→A | V241I (GTT→ATT) | yqhG → | DUF3828 family putative periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,158,370 | 0 | G | A | 97.8% | 141.6 / ‑4.0 | 46 | V241I (GTT→ATT) | yqhG | DUF3828 family putative periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (25/20); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01 | |||||||||||
TTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCGTTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAA > NC_000913/3158248‑3158496 | ttAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTc < 1:900277/135‑1 (MQ=255)ttAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTc > 2:900277/1‑135 (MQ=255) cTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTc > 1:1108571/1‑125 (MQ=255) cTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGaaa > 1:321966/1‑139 (MQ=255) cTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGAAGAAATTc < 2:1108571/125‑1 (MQ=255) caccTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGAt < 2:321966/139‑1 (MQ=255) ccTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTc < 2:234207/119‑1 (MQ=255) ccTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTc > 1:234207/1‑119 (MQ=255) ccTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTc > 2:294358/1‑139 (MQ=255) ccTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTc > 2:217265/1‑139 (MQ=255) ttCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTccg > 2:676536/1‑139 (MQ=255) aCGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCa < 1:217265/139‑1 (MQ=255) gCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGa < 2:1669862/105‑1 (MQ=255) gCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGa > 1:1669862/1‑105 (MQ=255) ccGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTccgc > 2:1381774/1‑121 (MQ=255) ccGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTccgc < 1:1381774/121‑1 (MQ=255) gATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGa > 1:844044/1‑139 (MQ=255) gCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAAt < 1:294358/139‑1 (MQ=255) aGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGcaac > 2:857301/1‑139 (MQ=255) aaCGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGaa > 2:1421087/1‑85 (MQ=255) aaCGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGaa < 1:1421087/85‑1 (MQ=255) ggCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGa < 1:857301/139‑1 (MQ=255) tttatttaCGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCt > 1:1445153/1‑116 (MQ=255) tttatttaCGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCt < 2:1445153/116‑1 (MQ=255) cAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGttt > 1:32038/1‑139 (MQ=255) gATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAg < 1:676536/139‑1 (MQ=255) aTCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAg > 1:186222/1‑49 (MQ=255) aTCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAg < 2:186222/49‑1 (MQ=255) ttGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGa > 2:270722/1‑139 (MQ=255) gagTCGCTATTTTCACGCGTTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAGCAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGt > 1:107752/1‑139 (MQ=255) gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGAt > 2:968768/1‑54 (MQ=255) gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGAt < 1:968768/54‑1 (MQ=255) gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTccgcc > 2:245215/1‑60 (MQ=255) gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTccgcc < 1:245215/60‑1 (MQ=255) gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCAt > 2:200657/1‑65 (MQ=255) gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCAt < 1:200657/65‑1 (MQ=255) gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGca < 1:936157/94‑1 (MQ=255) gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGca > 2:936157/1‑94 (MQ=255) gCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGaa > 1:1399781/1‑139 (MQ=255) cTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAgg < 2:141785/128‑1 (MQ=255) cTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAgg > 1:141785/1‑128 (MQ=255) cTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGaaa > 2:1435156/1‑139 (MQ=255) ttCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGa > 1:370093/1‑90 (MQ=255) ttCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGa < 2:370093/90‑1 (MQ=255) tCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGa < 1:704522/98‑1 (MQ=255) tCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGa > 2:704522/1‑98 (MQ=255) | TTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCGTTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAA > NC_000913/3158248‑3158496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |