Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	300,314	C→T	W207* (TGG→TGA)	paoC ←	PaoABC aldehyde oxidoreductase, Moco‑containing subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	300,314	0	C	T	100.0%	225.0 / NA	69	W207* (TGG→TGA)	paoC	PaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/36);  total (33/36)

GCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACC  >  NC_000913/300180‑300448                                                                                                                                       |                                                                                                                                       gcATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCaga                                                                                                                                    >  2:1344254/1‑139 (MQ=255)     cGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGt                                                                                                                                <  1:1344254/139‑1 (MQ=255)          ctcAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCt                                                                                                                           <  2:606485/139‑1 (MQ=255)          ctcAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCt                                                                                                                           <  1:1572635/139‑1 (MQ=255)                  cAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCa                                                                                                                   <  2:318813/139‑1 (MQ=255)                     cTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGt                                                                                                                                >  2:3247/1‑123 (MQ=255)                     cTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGt                                                                                                                                <  1:3247/123‑1 (MQ=255)                     cTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCt                                                                                                                           >  2:1270962/1‑128 (MQ=255)                     cTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCt                                                                                                                           <  1:1270962/128‑1 (MQ=255)                     cTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATcc                                                                                                                <  1:1292378/139‑1 (MQ=255)                            ccAACCCCTCCTCCGATATACGGTGAGATAATACGCACATCCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACgg                                                                                                         <  2:1261238/139‑1 (MQ=255)                            ccAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACgg                                                                                                         <  2:649512/139‑1 (MQ=255)                                      cgccgATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACgg                                                                                                         <  2:694646/129‑1 (MQ=255)                                      cgccgATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACgg                                                                                                         >  1:694646/1‑129 (MQ=255)                                            tataCGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCgg                                                                                         <  1:739812/139‑1 (MQ=255)                                                   ggAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATc                                                                                  >  1:1002250/1‑139 (MQ=255)                                                     agaTAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCa                                                                                              >  2:192283/1‑125 (MQ=255)                                                     agaTAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCa                                                                                              <  1:192283/125‑1 (MQ=255)                                                      gaTAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTc                                                                                                                     <  2:1143501/101‑1 (MQ=255)                                                      gaTAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTc                                                                                                                     >  1:1143501/1‑101 (MQ=255)                                                      gaTAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCa                                                                                    >  2:234110/1‑134 (MQ=255)                                                      gaTAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCa                                                                                    <  1:234110/134‑1 (MQ=255)                                                       ataataCGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCa                                                                              >  2:1169664/1‑139 (MQ=255)                                                        taataCGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCat                                                                             >  1:1202195/1‑139 (MQ=255)                                                        taataCGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCat                                                                             >  2:838159/1‑139 (MQ=255)                                                             cGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTa                                                                                                                               >  2:759351/1‑84 (MQ=255)                                                             cGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTa                                                                                                                               <  1:759351/84‑1 (MQ=255)                                                               cacaTTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGctc                                                                      >  2:996205/1‑139 (MQ=255)                                                                  aTTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCAt                                                                                   <  1:627268/123‑1 (MQ=255)                                                                  aTTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCAt                                                                                   >  2:627268/1‑123 (MQ=255)                                                                   ttCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGt                                                                  >  1:347691/1‑139 (MQ=255)                                                                    tctcCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCa                                                                                                                                      >  2:1099425/1‑70 (MQ=255)                                                                    tctcCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTc                                                                 >  2:22575/1‑139 (MQ=255)                                                                      tcCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAgg                                                                                                >  1:95790/1‑106 (MQ=255)                                                                      tcCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAgg                                                                                                <  2:95790/106‑1 (MQ=255)                                                                      tcCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCg                                                               <  1:22575/139‑1 (MQ=255)                                                                        cACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCggg                                                             <  1:996205/139‑1 (MQ=255)                                                                        cACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCggg                                                             <  1:838159/139‑1 (MQ=255)                                                                          cGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCgg                                                                                         <  2:631662/109‑1 (MQ=255)                                                                          cGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCgg                                                                                         >  1:631662/1‑109 (MQ=255)                                                                            ggAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCggg                                                             <  1:565860/135‑1 (MQ=255)                                                                            ggAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCggg                                                             >  2:565860/1‑135 (MQ=255)                                                                              aaCTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCgtg                                                       >  1:659888/1‑139 (MQ=255)                                                                                       cGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTAt                                                                                                                        <  2:720038/65‑1 (MQ=255)                                                                                       cGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTAt                                                                                                                        >  1:720038/1‑65 (MQ=255)                                                                                       cGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCggg                                                             >  1:278140/1‑124 (MQ=255)                                                                                       cGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCggg                                                             <  2:278140/124‑1 (MQ=255)                                                                                           tttGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCaa                                          >  2:694053/1‑139 (MQ=255)                                                                                           tttGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCaa                                          >  2:197335/1‑139 (MQ=255)                                                                                                   aTCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCggg                                                             <  1:586774/112‑1 (MQ=255)                                                                                                   aTCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCggg                                                             >  2:586774/1‑112 (MQ=255)                                                                                                       gTGCGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAg                              <  1:1169664/139‑1 (MQ=255)                                                                                                          cGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCgg                                                                                         >  2:1524398/1‑77 (MQ=255)                                                                                                          cGGCACCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCgg                                                                                         <  1:1524398/77‑1 (MQ=255)                                                                                                             cACCAGTCAATCATCTGATTTGAGGTTCAGCGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCAGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACTGCCGCGGAgg                        >  2:646262/1‑139 (MQ=255)                                                                                                              aCCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACgg                                                                                                         <  2:89019/57‑1 (MQ=255)                                                                                                              aCCAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACgg                                                                                                         >  1:89019/1‑57 (MQ=255)                                                                                                                cAGTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGa                     <  2:1002250/139‑1 (MQ=255)                                                                                                                  gTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCa                                           <  1:514898/115‑1 (MQ=255)                                                                                                                  gTCAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCa                                           >  2:514898/1‑115 (MQ=255)                                                                                                                    cAATCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGc                 <  2:1202195/139‑1 (MQ=255)                                                                                                                     aaTCATCTGATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGcc                <  2:659888/139‑1 (MQ=255)                                                                                                                             gATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAAt                                         <  1:770320/106‑1 (MQ=255)                                                                                                                             gATTTGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAAt                                         >  2:770320/1‑106 (MQ=255)                                                                                                                                ttGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCggg                                                             <  2:1106145/83‑1 (MQ=255)                                                                                                                                ttGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCAGCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCggg                                                             >  1:1106145/1‑83 (MQ=255)                                                                                                                                 tGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCgtg                                                       <  1:1211922/88‑1 (MQ=255)                                                                                                                                 tGAGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGGCCGGGGTCgtg                                                       >  2:1211922/1‑88 (MQ=255)                                                                                                                                   aGGTTCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCAcc  <  1:694053/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACC  >  NC_000913/300180‑300448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑