Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,277,726	C→T	F130F (TTC→TTT)	yhaV →	toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,277,726	0	C	T	100.0%	188.8 / NA	58	F130F (TTC→TTT)	yhaV	toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/27);  total (31/27)

GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTC  >  NC_000913/3277590‑3277861                                                                                                                                         |                                                                                                                                        gggTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAg                                                                                                                                       >  1:470547/1‑139 (MQ=255)  ggTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGc                                                                                                                                      >  2:1354529/1‑139 (MQ=255)                    tCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGa                                                                                                                    <  1:869187/139‑1 (MQ=255)                        tATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACAtc                                                                                                                >  2:1003471/1‑139 (MQ=255)                              ctctTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTg                                                                                                          <  2:470547/139‑1 (MQ=255)                                     ttCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTg                                                                                                   >  1:502919/1‑139 (MQ=255)                                       cGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                                                                                                 <  1:1354529/139‑1 (MQ=255)                                             aGTGTAAAAGAGAAAGTCATCATTCTGGGATGGATGACCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGt                                                                                                                               <  1:1258230/103‑1 (MQ=255)                                             aGTGAAAAAGAGAAAGTCATCATTCTGGGCTGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGt                                                                                                                               >  2:1258230/1‑103 (MQ=255)                                               tGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                                                         <  2:502919/139‑1 (MQ=255)                                                      gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCgag                                                                                  <  1:1003471/139‑1 (MQ=255)                                                         aaaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAag                                                                                                                        <  1:1088127/98‑1 (MQ=255)                                                         aaaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAag                                                                                                                        >  2:1088127/1‑98 (MQ=255)                                                           aGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTTCCGACTGGGAAACCCTCACCCGAGAAACa                                                                             >  2:289356/1‑139 (MQ=255)                                                                tcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACagaaga                                                                        <  1:541263/139‑1 (MQ=255)                                                                           tggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGaaa                                                                               <  2:1306944/121‑1 (MQ=255)                                                                           tggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGaaa                                                                               >  1:1306944/1‑121 (MQ=255)                                                                             gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACa                                                                                                                  >  2:1474521/1‑84 (MQ=255)                                                                             gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACa                                                                                                                  <  1:1474521/84‑1 (MQ=255)                                                                             gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                                                      <  2:881217/128‑1 (MQ=255)                                                                             gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                                                      >  1:881217/1‑128 (MQ=255)                                                                             gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAACCATTGATgg                                                           >  1:556800/1‑139 (MQ=255)                                                                              atgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCg                                                                                                       >  2:785028/1‑94 (MQ=255)                                                                              atgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCg                                                                                                       <  1:785028/94‑1 (MQ=255)                                                                                        aaaaCACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATg                                                >  2:329480/1‑139 (MQ=255)                                                                                                ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                                                                                                 <  1:589830/82‑1 (MQ=255)                                                                                                ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                                                                                                 <  2:79417/82‑1 (MQ=255)                                                                                                ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                                                                                                 >  1:79417/1‑82 (MQ=255)                                                                                                ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                                                                                                 >  2:589830/1‑82 (MQ=255)                                                                                                ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGAt                                                             <  1:928018/118‑1 (MQ=255)                                                                                                ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGAt                                                             >  2:928018/1‑118 (MQ=255)                                                                                                ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTCAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGa                                        >  2:979347/1‑139 (MQ=255)                                                                                                       ccTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAtt                                 >  1:1009803/1‑139 (MQ=255)                                                                                                         tACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCg                                                                                                       <  1:433266/67‑1 (MQ=255)                                                                                                         tACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCg                                                                                                       >  2:433266/1‑67 (MQ=255)                                                                                                         tACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGa                                                              <  1:1340323/108‑1 (MQ=255)                                                                                                         tACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGa                                                              >  2:1340323/1‑108 (MQ=255)                                                                                                         tACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCg                               >  1:648311/1‑139 (MQ=255)                                                                                                           cGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGaaa                                                                               <  2:656274/89‑1 (MQ=255)                                                                                                           cGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGaaa                                                                               >  1:656274/1‑89 (MQ=255)                                                                                                           cGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCgtg                             >  2:254281/1‑139 (MQ=255)                                                                                                                 aaaaaCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGAcc                       <  2:648311/139‑1 (MQ=255)                                                                                                                         aTGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCa               <  1:329480/139‑1 (MQ=255)                                                                                                                             cTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCt           <  1:979347/139‑1 (MQ=255)                                                                                                                                  ccGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTtctc      <  2:1009803/139‑1 (MQ=255)                                                                                                                                  ccGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTtctc      <  2:28947/139‑1 (MQ=255)                                                                                                                                    gTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCAc    >  2:933118/1‑139 (MQ=255)                                                                                                                                     tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                                                                                                 >  1:1355258/1‑45 (MQ=255)                                                                                                                                     tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                                                                                                 <  2:1355258/45‑1 (MQ=255)                                                                                                                                     tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt                                                                                          >  2:1681481/1‑52 (MQ=255)                                                                                                                                     tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt                                                                                          <  1:1681481/52‑1 (MQ=255)                                                                                                                                     tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAAc                                                                              >  1:620321/1‑64 (MQ=255)                                                                                                                                     tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAAc                                                                              <  2:620321/64‑1 (MQ=255)                                                                                                                                     tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAt                                  >  2:818320/1‑108 (MQ=255)                                                                                                                                     tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAt                                  <  1:818320/108‑1 (MQ=255)                                                                                                                                     tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACt   >  2:798862/1‑139 (MQ=255)                                                                                                                                     tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACt   >  1:102903/1‑139 (MQ=255)                                                                                                                                      aTTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTc  >  2:221299/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                        GGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTC  >  NC_000913/3277590‑3277861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑