Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	312,189	C→T	G26G (GGC→GGT)	ykgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	312,189	0	C	T	100.0%	208.5 / NA	65	G26G (GGC→GGT)	ykgL	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/32);  total (33/32)

TTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACA  >  NC_000913/312056‑312318                                                                                                                                      |                                                                                                                                  tttATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGc                                                                                                                              >  1:1229237/1‑139 (MQ=255)             ccAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGt                                                                                                                  <  1:1282101/139‑1 (MQ=255)                aCAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCaa                                                                                                               >  2:128546/1‑139 (MQ=255)                 cAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTc                                                                                                                 >  1:1035005/1‑136 (MQ=255)                 cAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTc                                                                                                                 <  2:1035005/136‑1 (MQ=255)                    aTTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACAtt                                                                                                           >  1:368535/1‑139 (MQ=255)                    aTTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACAtt                                                                                                           >  1:80080/1‑139 (MQ=255)                     tttACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGc                                                                                                                              <  1:250656/119‑1 (MQ=255)                     tttACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGc                                                                                                                              >  2:250656/1‑119 (MQ=255)                     tttACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTcaa                                                                                                                       <  2:299269/126‑1 (MQ=255)                     tttACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTcaa                                                                                                                       >  1:299269/1‑126 (MQ=255)                         ccACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTTAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGAt                                                                                                      >  2:395087/1‑139 (MQ=255)                                    atcTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATAtt                                                                                           >  2:1421864/1‑139 (MQ=255)                                      cTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCg                                                                                         <  1:1457686/139‑1 (MQ=255)                                         aaCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTg                                                                                                                            >  1:873896/1‑101 (MQ=255)                                         aaCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTg                                                                                                                            <  2:873896/101‑1 (MQ=255)                                         aaCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGATGAAGCTGGTCAACAAGt                                                                                                                  <  1:1113650/111‑1 (MQ=255)                                         aaCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGCGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGt                                                                                                                  >  2:1113650/1‑111 (MQ=255)                                               ttGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACAt                                                                                                            >  2:1485191/1‑111 (MQ=255)                                               ttGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACAt                                                                                                            <  1:1485191/111‑1 (MQ=255)                                                tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTc                                                                                                                 <  2:587279/105‑1 (MQ=255)                                                tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTc                                                                                                                 >  1:587279/1‑105 (MQ=255)                                                tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGgagag                                                                                    <  1:1173123/134‑1 (MQ=255)                                                tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGgagag                                                                                    >  2:1173123/1‑134 (MQ=255)                                                tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACg                                                                               >  1:1635478/1‑139 (MQ=255)                                                    tGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAg                                                                           >  2:138813/1‑139 (MQ=255)                                                     gAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAgg                                                                          <  1:138813/139‑1 (MQ=255)                                                                cTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGa                                                                                                >  2:298379/1‑106 (MQ=255)                                                                cTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGa                                                                                                <  1:298379/106‑1 (MQ=255)                                                                 tGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCa                                                              >  1:771664/1‑139 (MQ=255)                                                                        cAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGt                                                                                                                  <  1:356592/80‑1 (MQ=255)                                                                        cAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGt                                                                                                                  >  2:356592/1‑80 (MQ=255)                                                                        cAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                       <  2:368535/139‑1 (MQ=255)                                                                        cAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                       <  1:1421864/139‑1 (MQ=255)                                                                                ggagACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTcaa                                                                                                                       >  2:1566764/1‑67 (MQ=255)                                                                                ggagACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTcaa                                                                                                                       <  1:1566764/67‑1 (MQ=255)                                                                                  agaCTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTcaac                                                                                                                      <  2:853295/66‑1 (MQ=255)                                                                                  agaCTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTcaac                                                                                                                      >  1:853295/1‑66 (MQ=255)                                                                                   gaCTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGaaa                                                                                 >  2:214548/1‑102 (MQ=255)                                                                                   gaCTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGaaa                                                                                 <  1:214548/102‑1 (MQ=255)                                                                                       gAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGc                                                                                                                              <  1:989110/53‑1 (MQ=255)                                                                                       gAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGc                                                                                                                              >  2:989110/1‑53 (MQ=255)                                                                                       gAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATcc                                        >  2:1401490/1‑139 (MQ=255)                                                                                       gAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATcc                                        >  1:383982/1‑139 (MQ=255)                                                                                       gAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATAGATcc                                        >  1:1114301/1‑139 (MQ=255)                                                                                        aGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTc                                                                                                                         <  2:486026/57‑1 (MQ=255)                                                                                        aGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTc                                                                                                                         >  1:486026/1‑57 (MQ=255)                                                                                           ggCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAg                                    <  1:128546/139‑1 (MQ=255)                                                                                             cGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGc                                                                  >  1:1333306/1‑107 (MQ=255)                                                                                             cGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGc                                                                  <  2:1333306/107‑1 (MQ=255)                                                                                                           aaGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCaaa                                                            >  2:133594/1‑99 (MQ=255)                                                                                                           aaGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCaaa                                                            <  1:133594/99‑1 (MQ=255)                                                                                                           aaGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGgt                    <  1:1401490/139‑1 (MQ=255)                                                                                                              agGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGtt                 <  2:771664/139‑1 (MQ=255)                                                                                                              agGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGtt                 <  2:1229237/139‑1 (MQ=255)                                                                                                                ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATg                         <  1:395693/129‑1 (MQ=255)                                                                                                                ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATg                         >  2:395693/1‑129 (MQ=255)                                                                                                                  ttGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATaa             >  2:1374609/1‑139 (MQ=255)                                                                                                                    gATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                       >  1:1688948/1‑95 (MQ=255)                                                                                                                    gATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                       <  2:1688948/95‑1 (MQ=255)                                                                                                                        ccAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCACATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacacac       <  2:1114301/139‑1 (MQ=255)                                                                                                                        ccAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacacac       <  2:1635478/139‑1 (MQ=255)                                                                                                                          aGCATACAGGGGTGAAGCTGATCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacacacta     >  2:992837/1‑137 (MQ=255)                                                                                                                             aTACAGGGGTGAAGCTGGTCACCAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACa  <  1:992837/139‑1 (MQ=255)                                                                                                                             aTACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACa  <  2:383982/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  TTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACA  >  NC_000913/312056‑312318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑