| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,330,396 | G→A | *478* (TAG→TAA) | dacB → | D‑alanyl‑D‑alanine carboxypeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,330,396 | 0 | G | A | 100.0% | 109.6 / NA | 35 | *478* (TAG→TAA) | dacB | D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/17); total (18/17) | |||||||||||
TCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCA > NC_000913/3330294‑3330510 | tCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCa > 2:293161/1‑139 (MQ=255) ttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac‑a < 2:950250/122‑1 (MQ=255) ttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac‑a > 1:950250/1‑122 (MQ=255) aGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACaa < 2:1418910/133‑1 (MQ=255) aGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACaa > 1:1418910/1‑133 (MQ=255) gAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTgcgc < 1:293161/139‑1 (MQ=255) aaCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt < 2:275267/139‑1 (MQ=255) ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCttt > 1:1300649/1‑139 (MQ=255) tGCGGATCAGCGTAAACGCCATATTCCGTTAGTGCGTTTTTGAAACCCATTGGTAAAAGGAAATTTATCAGAACAATAAAT‑AAAAAAAAAACCCAGCACAATGGCCGGGGCAGCAGATAATTAACaaaatcagcttaga > 2:56998/1‑128 (MQ=255) gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCg < 2:937484/106‑1 (MQ=255) gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCg > 1:937484/1‑106 (MQ=255) ggATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGt > 2:566919/1‑127 (MQ=255) ggATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGt < 1:566919/127‑1 (MQ=255) gTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac < 1:1692558/80‑1 (MQ=255) gTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac > 2:1692558/1‑80 (MQ=255) ttCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCtt < 1:1415803/113‑1 (MQ=255) ttCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCtt > 2:1415803/1‑113 (MQ=255) cgtttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTGATCAAAAAGa > 2:1198839/4‑55 (MQ=255) cgtttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa < 1:1198839/52‑1 (MQ=255) cgtttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGatta < 2:1023465/83‑1 (MQ=255) cgtttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGatta > 1:1023465/4‑86 (MQ=255) tttGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCa > 1:778556/1‑139 (MQ=255) ttGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCaa > 2:418710/1‑139 (MQ=255) tGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCaaa < 1:418710/139‑1 (MQ=255) taAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa < 2:778556/139‑1 (MQ=255) tatTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac > 1:603170/1‑39 (MQ=38) tatTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac < 2:603170/39‑1 (MQ=38) tttATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTc > 2:29169/1‑51 (MQ=255) tttATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTc < 1:29169/51‑1 (MQ=255) tttATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATg < 2:1407600/88‑1 (MQ=255) tttATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATg > 1:1407600/1‑88 (MQ=255) aaCAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTc > 2:228560/1‑43 (MQ=255) aaCAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTc < 1:228560/43‑1 (MQ=255) cAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt < 1:686184/62‑1 (MQ=255) cAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt > 2:686184/1‑62 (MQ=255) | TCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCA > NC_000913/3330294‑3330510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |