Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,434,577 G→A A122T (GCA→ACA)  fmt → 10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,434,5770GA100.0% 189.9 / NA 59A122T (GCA→ACA) fmt10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/31);  total (28/31)

GTCGCCGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTGCACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGT  >  NC_000913/3434445‑3434711
                                                                                                                                    |                                                                                                                                      
gTCGCCGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCaa                                                                                                                                  >  1:262222/1‑139 (MQ=255)
       tataagagacagATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                           <  1:306363/128‑1 (MQ=255)
       aaCTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                           <  1:529615/139‑1 (MQ=255)
       aaCTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                           <  2:44572/139‑1 (MQ=255)
       aaCTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                           >  2:529615/1‑139 (MQ=255)
       aaCTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                           <  2:624634/139‑1 (MQ=255)
             aGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACt                                                                                                                     <  2:262222/139‑1 (MQ=255)
                 tGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAAACCAACGCTCACTATgg                                                                                                                 >  2:1541104/1‑139 (MQ=255)
                      ttATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCggg                                                                                                            <  2:340527/139‑1 (MQ=255)
                        aTGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTg                                                                                                          <  2:378306/139‑1 (MQ=255)
                             cgtcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATggg                                                                                                                >  2:1217456/1‑128 (MQ=255)
                             cgtcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATggg                                                                                                                <  1:1217456/128‑1 (MQ=255)
                              gtcggcgCCTATGGTTTAATTCTGCCGAAAGTAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCGCTGCTGCCACGCTGGCGCGTTGCTACACCAATCCAAGGCTCACTATGGGctgtctcttata                                                                                                    >  2:306363/1‑128 (MQ=255)
                                cgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                           >  2:408600/1‑114 (MQ=255)
                                cgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACg                                                                                                                           <  1:408600/114‑1 (MQ=255)
                                 gtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGaaa                                                                                                 <  2:1262171/139‑1 (MQ=255)
                                              tAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCAt                                                                                    <  2:799489/139‑1 (MQ=255)
                                               aaTTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCAtt                                                                                   >  1:1068290/1‑139 (MQ=255)
                                                     gCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGcc                                                                                                                         >  2:1263883/1‑94 (MQ=255)
                                                     gCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCt                                                                                                                         <  1:1263883/95‑1 (MQ=255)
                                                     gCCGAAAGCAGTGCTGGAGATGCCGCATCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCaa                                                                             >  1:404052/1‑139 (MQ=255)
                                                           aGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGAt                                                                       >  2:544454/1‑139 (MQ=255)
                                                                 gCTTGAGATGCCGCGTCTTGGCTGTATCCACGTTCATGGTTCACTGCTGCCCCGCTGGCGCGGTGCTACCCCAATCCAACGCTCAATATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGt                                                                 <  1:1541104/139‑1 (MQ=255)
                                                                        aTGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGaca                                                          >  2:32966/1‑139 (MQ=255)
                                                                          gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATcc                                                                                                                               >  1:930648/1‑68 (MQ=255)
                                                                          gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATcc                                                                                                                               <  2:930648/68‑1 (MQ=255)
                                                                          gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                          >  1:1277847/1‑105 (MQ=255)
                                                                          gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                          >  1:1282337/1‑105 (MQ=255)
                                                                          gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                          <  2:1277847/105‑1 (MQ=255)
                                                                          gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                          <  2:1282337/105‑1 (MQ=255)
                                                                          gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACAcc                                                        >  1:692101/1‑139 (MQ=255)
                                                                          gCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTACCACGCTGGCGCGGCGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGGAAATGGATGACGGTTTAGACAcc                                                        >  2:1116591/1‑139 (MQ=255)
                                                                            cgcgTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCgg                                                      >  2:35662/1‑139 (MQ=255)
                                                                                  ttGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGatat                                                <  2:404052/139‑1 (MQ=255)
                                                                                   tGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATg                                               <  1:544454/139‑1 (MQ=255)
                                                                                    ggCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGc                                              <  2:692101/139‑1 (MQ=255)
                                                                                     gCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGct                                             >  2:114439/1‑139 (MQ=255)
                                                                                         tATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGAt                                                                                                        <  1:1203416/76‑1 (MQ=255)
                                                                                         tATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGAt                                                                                                        >  2:1203416/1‑76 (MQ=255)
                                                                                                   cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCaa                                                                                                                             >  2:604603/1‑45 (MQ=255)
                                                                                                   cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCaa                                                                                                                             <  1:604603/45‑1 (MQ=255)
                                                                                                   cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGc                                                                                                      >  2:136514/1‑68 (MQ=255)
                                                                                                   cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGc                                                                                                      <  1:1332714/68‑1 (MQ=255)
                                                                                                   cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGc                                                                                                      <  1:136514/68‑1 (MQ=255)
                                                                                                   cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGc                                                                                                      >  2:1332714/1‑68 (MQ=255)
                                                                                                   cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCa                                                                                     <  2:174998/85‑1 (MQ=255)
                                                                                                   cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCa                                                                                     >  1:174998/1‑85 (MQ=255)
                                                                                                           aCTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCAt                                                                                    <  2:999398/78‑1 (MQ=255)
                                                                                                           aCTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCAt                                                                                    >  1:999398/1‑78 (MQ=255)
                                                                                                           aCTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGAtt                       >  2:880280/1‑139 (MQ=255)
                                                                                                             tgctgcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGc                             <  1:20276/131‑1 (MQ=255)
                                                                                                             tgctgcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGc                             >  2:20276/1‑131 (MQ=255)
                                                                                                              gctgcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATg                                                                      <  1:173934/89‑1 (MQ=255)
                                                                                                              gctgcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATg                                                                      >  2:173934/1‑89 (MQ=255)
                                                                                                                 gcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCa                 <  1:35662/139‑1 (MQ=255)
                                                                                                                 gcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCa                 <  2:1068290/139‑1 (MQ=255)
                                                                                                                       cTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGAt           >  1:88715/1‑139 (MQ=255)
                                                                                                                         ggCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATAc         <  1:32966/139‑1 (MQ=255)
                                                                                                                              ggTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTg    <  2:88715/139‑1 (MQ=255)
                                                                                                                                tgctacACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                          >  1:1385717/1‑51 (MQ=255)
                                                                                                                                tgctacACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                          <  2:1385717/51‑1 (MQ=255)
                                                                                                                                tgctacACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGt  >  1:737620/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                      
GTCGCCGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTGCACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGT  >  NC_000913/3434445‑3434711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑