Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	335,970	C→T	Q16* (CAA→TAA)	yahE →	DUF2877 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	335,970	0	C	T	100.0%	215.4 / NA	66	Q16* (CAA→TAA)	yahE	DUF2877 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/30);  total (36/30)

GGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTTGAT  >  NC_000913/335839‑336104                                                                                                                                    |                                                                                                                                       ggggCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACa                                                                                                                                 >  2:966137/1‑139 (MQ=255)               aTTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                                    >  2:304478/1‑122 (MQ=255)               aTTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                                    <  1:304478/122‑1 (MQ=255)                     cAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                             <  1:597948/139‑1 (MQ=255)                          aCTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCg                                                                                                        >  1:1313977/1‑139 (MQ=255)                           cTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCgg                                                                                                       >  2:1276409/1‑139 (MQ=255)                                  ccGCAGGTGCATACACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAatat                                                                                                >  2:119461/1‑139 (MQ=255)                                  ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAatat                                                                                                >  2:1507415/1‑139 (MQ=255)                                   cGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTc                                                                                                                    >  2:493604/1‑118 (MQ=255)                                   cGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTc                                                                                                                    <  1:493604/118‑1 (MQ=255)                                    gCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCg                                                                                              <  1:966137/139‑1 (MQ=255)                                     cAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGc                                                                                             >  2:1652267/1‑139 (MQ=255)                                          gCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATAACGCACTcc                                                                                        >  1:1006355/1‑139 (MQ=255)                                             tAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCgg                                                                                     >  2:1430359/1‑139 (MQ=255)                                                   gggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTaaa                                                                                                   <  2:930293/119‑1 (MQ=255)                                                   gggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTaaa                                                                                                   >  1:930293/1‑119 (MQ=255)                                                    ggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGaa                                                                                                                          >  1:46564/1‑95 (MQ=255)                                                    ggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGaa                                                                                                                          <  2:46564/95‑1 (MQ=255)                                                    ggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCaga                                                                                                           >  1:207216/1‑110 (MQ=255)                                                    ggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCaga                                                                                                           <  2:207216/110‑1 (MQ=255)                                                     gAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCcag                                                                             >  1:1362454/1‑139 (MQ=255)                                                     gAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGAGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCcag                                                                             >  2:1213206/1‑139 (MQ=255)                                                            gCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGCAAATATCGCACTCCCGGCTCGCCAGCAGTTGc                                                                      <  2:1006355/139‑1 (MQ=255)                                                                    cacacCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgctg                                                              >  1:599838/1‑139 (MQ=255)                                                                         cAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGt                                                                                                                     >  1:984664/1‑79 (MQ=255)                                                                         cAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAAAAGGt                                                                                                                     <  2:984664/79‑1 (MQ=255)                                                                          aGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgt                                                        >  2:691867/1‑139 (MQ=255)                                                                           gAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                                       <  1:1430359/139‑1 (MQ=255)                                                                           gAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                                       <  1:1515577/139‑1 (MQ=255)                                                                                 cGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgct                                                               >  2:668772/1‑125 (MQ=255)                                                                                 cGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgct                                                               <  1:668772/125‑1 (MQ=255)                                                                                          tgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCg                                                                                                        >  2:964079/1‑75 (MQ=255)                                                                                          tgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCg                                                                                                        <  1:964079/75‑1 (MQ=255)                                                                                          tgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                                       <  2:1434619/124‑1 (MQ=255)                                                                                          tgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                                       >  1:1434619/1‑124 (MQ=255)                                                                                              cgcTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTaaa                                                                                                   >  2:190549/1‑76 (MQ=255)                                                                                              cgcTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTaaa                                                                                                   <  1:190549/76‑1 (MQ=255)                                                                                                cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                                    <  1:1080042/41‑1 (MQ=255)                                                                                                cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                                    >  2:1080042/1‑41 (MQ=255)                                                                                                cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAAt                                         >  1:894404/1‑132 (MQ=255)                                                                                                cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAAt                                         <  2:894404/132‑1 (MQ=255)                                                                                                  aaCTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAAc                                >  2:641575/1‑139 (MQ=255)                                                                                                       cggatgcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgct                                                               >  2:424451/1‑103 (MQ=255)                                                                                                       cggatgcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgct                                                               <  1:424451/103‑1 (MQ=255)                                                                                                       cggatgcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTg                           <  1:1136896/139‑1 (MQ=255)                                                                                                            gcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAAc                                                                                                                         <  2:850501/40‑1 (MQ=255)                                                                                                            gcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAAc                                                                                                                         >  1:347129/1‑40 (MQ=39)                                                                                                            gcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAAc                                                                                                                         <  2:347129/40‑1 (MQ=255)                                                                                                            gcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAAc                                                                                                                         >  1:850501/1‑40 (MQ=39)                                                                                                             cggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGtt                     >  2:1126964/1‑139 (MQ=255)                                                                                                               gatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTccc                                                                                       >  2:1199379/1‑71 (MQ=255)                                                                                                               gatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTccc                                                                                       <  1:1199379/71‑1 (MQ=255)                                                                                                                 ttttGTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCt                                                                                   <  1:1222041/73‑1 (MQ=255)                                                                                                                 ttttCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCt                                                                                   >  2:1222041/1‑73 (MQ=255)                                                                                                                 ttttCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGca                 <  1:119461/139‑1 (MQ=255)                                                                                                                    tCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGcc                                                                               <  1:1236391/74‑1 (MQ=255)                                                                                                                    tCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGcc                                                                               <  1:1160614/74‑1 (MQ=255)                                                                                                                    tCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGcc                                                                               >  2:1160614/1‑74 (MQ=255)                                                                                                                    tCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGcc                                                                               >  2:1236391/1‑74 (MQ=255)                                                                                                                       ggCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACACTTGTCGGTTGGcactcact           <  1:1126964/139‑1 (MQ=255)                                                                                                                         cgcAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcactca         <  1:739362/139‑1 (MQ=255)                                                                                                                              cGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                                       <  2:278803/88‑1 (MQ=255)                                                                                                                              cGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                                       >  1:278803/1‑88 (MQ=255)                                                                                                                              cGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGaa                                                     <  2:686081/90‑1 (MQ=255)                                                                                                                              cGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGaa                                                     >  1:686081/1‑90 (MQ=255)                                                                                                                                ggggTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTtgat  >  2:1400633/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                       GGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACTTTGAT  >  NC_000913/335839‑336104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑