Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,731,980 G→A G284S (GGT→AGT)  xylF → D‑xylose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,731,9800GA100.0% 282.7 / NA 87G284S (GGT→AGT) xylFD‑xylose transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/43);  total (44/43)

AATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATGGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAG  >  NC_000913/3731850‑3732111
                                                                                                                                  |                                                                                                                                   
aaTCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGaa                                                                                                                             >  1:1675159/1‑139 (MQ=255)
             gATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                >  1:200344/1‑139 (MQ=255)
                       tcGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACca                                                                                                             <  1:435870/132‑1 (MQ=255)
                       tcGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACca                                                                                                             >  2:435870/1‑132 (MQ=255)
                         gCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaata                                                                                                    <  2:1675159/139‑1 (MQ=255)
                              tATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaataa                                                                                                   <  1:340895/135‑1 (MQ=255)
                              tATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaataa                                                                                                   >  2:340895/1‑135 (MQ=255)
                              tATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGc                                                                                               >  2:1271293/1‑139 (MQ=255)
                                  aaaCGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGa                                                                                           <  2:491081/139‑1 (MQ=255)
                                       tATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                >  1:1095858/1‑113 (MQ=255)
                                       tATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                <  2:1095858/113‑1 (MQ=255)
                                        aTTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                >  2:372732/1‑112 (MQ=255)
                                        aTTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                <  1:372732/112‑1 (MQ=255)
                                        aTTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATg                                                                                     >  1:928967/1‑139 (MQ=255)
                                          tgctgcCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTc                                                                                   >  2:646868/1‑139 (MQ=255)
                                                          aCTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGa                                                                                                                  <  2:598974/92‑1 (MQ=255)
                                                          aCTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGa                                                                                                                  >  1:598974/1‑92 (MQ=255)
                                                                aCGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACcga                                                             <  2:928967/139‑1 (MQ=255)
                                                                    tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           <  2:662860/121‑1 (MQ=255)
                                                                    tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           >  1:662860/1‑121 (MQ=255)
                                                                      tataAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGa                                                                                                                  >  2:1179278/1‑80 (MQ=255)
                                                                      tataAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGa                                                                                                                  <  1:1179278/80‑1 (MQ=255)
                                                                      tataAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                       >  1:491344/1‑139 (MQ=255)
                                                                              tATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                <  2:277482/74‑1 (MQ=255)
                                                                              tATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                >  1:277482/1‑74 (MQ=255)
                                                                              tATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaa                                               <  1:646868/139‑1 (MQ=255)
                                                                                ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGc                                                                                                                     >  1:330655/1‑67 (MQ=255)
                                                                                ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGc                                                                                                                     <  2:330655/67‑1 (MQ=255)
                                                                                ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACca                                                                                                             <  1:1021539/75‑1 (MQ=255)
                                                                                ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACca                                                                                                             >  2:1021539/1‑75 (MQ=255)
                                                                                ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACGCTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           >  2:1211172/1‑109 (MQ=255)
                                                                                ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGGCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaaaa                                             >  1:1072052/1‑139 (MQ=255)
                                                                                ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCt                                                                                             >  2:1068274/1‑91 (MQ=255)
                                                                                ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCt                                                                                             <  1:1068274/91‑1 (MQ=255)
                                                                                ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           <  2:1466128/109‑1 (MQ=255)
                                                                                ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           <  2:485159/109‑1 (MQ=255)
                                                                                ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           >  1:485159/1‑109 (MQ=255)
                                                                                ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           <  1:1211172/109‑1 (MQ=255)
                                                                                ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           >  1:1466128/1‑109 (MQ=255)
                                                                                  aCGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAg                                                                                                                      >  1:644035/1‑64 (MQ=255)
                                                                                  aCGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAg                                                                                                                      <  2:644035/64‑1 (MQ=255)
                                                                                      tgttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACAt                                       >  2:1344912/1‑139 (MQ=255)
                                                                                       gttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGt                                                                                                                                   <  2:497426/46‑1 (MQ=255)
                                                                                       gttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGt                                                                                                                                   >  1:497426/1‑46 (MQ=255)
                                                                                       gttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGa                                                                                                                  <  1:114834/63‑1 (MQ=255)
                                                                                       gttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGa                                                                                                                  >  2:114834/1‑63 (MQ=255)
                                                                                       gttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaataa                                                                                                   >  1:429063/1‑78 (MQ=255)
                                                                                       gttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaataa                                                                                                   <  2:429063/78‑1 (MQ=255)
                                                                                       gttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATc                                      <  2:491344/139‑1 (MQ=255)
                                                                                        ttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCa                                     >  2:801609/1‑139 (MQ=255)
                                                                                          ggCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAGcc                                                                                                                           <  2:1415586/51‑1 (MQ=255)
                                                                                          ggCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAAcc                                                                                                                           >  1:1415586/1‑51 (MQ=255)
                                                                                          ggCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           >  2:887575/1‑99 (MQ=255)
                                                                                          ggCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           <  1:887575/99‑1 (MQ=255)
                                                                                          ggCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                       <  2:81311/119‑1 (MQ=255)
                                                                                          ggCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                       >  1:81311/1‑119 (MQ=255)
                                                                                                tACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                >  1:1531945/1‑56 (MQ=255)
                                                                                                tACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                <  2:1531945/56‑1 (MQ=255)
                                                                                                tACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGACACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACg                             <  2:200344/139‑1 (MQ=255)
                                                                                                  cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           >  2:306326/1‑91 (MQ=255)
                                                                                                  cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           <  1:306326/91‑1 (MQ=255)
                                                                                                  cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCaa                                    <  1:1024369/130‑1 (MQ=255)
                                                                                                  cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGAGGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCaa                                    >  2:1024369/1‑130 (MQ=255)
                                                                                                     ccGCAGAAATTTCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           <  2:1214237/88‑1 (MQ=255)
                                                                                                     ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                         >  1:1146905/1‑42 (MQ=255)
                                                                                                     ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                <  2:1426332/51‑1 (MQ=255)
                                                                                                     ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                >  1:1426332/1‑51 (MQ=255)
                                                                                                     ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           >  1:1214237/1‑88 (MQ=255)
                                                                                                     ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                       >  2:1154957/1‑108 (MQ=255)
                                                                                                     ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                       <  1:1154957/108‑1 (MQ=255)
                                                                                                     ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAaaca                                           >  2:473929/1‑120 (MQ=255)
                                                                                                     ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAaaca                                           <  1:473929/120‑1 (MQ=255)
                                                                                                     ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAAt                        >  1:1468387/1‑139 (MQ=255)
                                                                                                     ccGCAGAAATTGCCGTTCAGTTGGGCAATAGTCAGGAACCaa                                                                                                                         <  2:1146905/42‑1 (MQ=38)
                                                                                                        cAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACt                                                                                                         <  2:829056/55‑1 (MQ=255)
                                                                                                        cAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACt                                                                                                         >  1:829056/1‑55 (MQ=255)
                                                                                                        cAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaaaa                                             <  1:641015/115‑1 (MQ=255)
                                                                                                        cAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaaaa                                             >  2:641015/1‑115 (MQ=255)
                                                                                                         aGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGc                                                                                                                     <  1:995441/42‑1 (MQ=255)
                                                                                                         aGAAATTGCCGTTGAGTTGGACAATAGTCAGGAACCAAAAGc                                                                                                                     >  2:995441/1‑42 (MQ=255)
                                                                                                          gAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAg                   <  2:1468387/139‑1 (MQ=255)
                                                                                                          gAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAg                   <  1:801609/139‑1 (MQ=255)
                                                                                                                gCCGTTGAGTTGGGCAATAGTCAGGAGCCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACTGAt             <  1:1271293/139‑1 (MQ=255)
                                                                                                                     tGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           >  1:920707/1‑72 (MQ=255)
                                                                                                                     tGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                           <  2:920707/72‑1 (MQ=255)
                                                                                                                        gTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCACCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACaaa     <  2:1072052/139‑1 (MQ=255)
                                                                                                                           gggCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAagag  >  2:1188141/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                   
AATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATGGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAG  >  NC_000913/3731850‑3732111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑