Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,819,488 C→T H205H (CAC→CAT
*561* (TAG→TAA
yicG →
ligB ←
UPF0126 family inner membrane protein
DNA ligase, NAD(+)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,819,4880CT100.0% 186.0 / NA 57H205H (CAC→CAT
*561* (TAG→TAA
yicG
ligB
UPF0126 family inner membrane protein
DNA ligase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/28);  total (29/28)

ACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAAC  >  NC_000913/3819367‑3819617
                                                                                                                         |                                                                                                                                 
acTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCtgt                                                                                                                  <  1:863144/139‑1 (MQ=255)
  tATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGa                                                                                                                >  2:1527717/1‑139 (MQ=255)
   aTGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGAt                                                                                                               <  1:510560/139‑1 (MQ=255)
              cATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCtgtg                                                                                                                 <  2:1262848/126‑1 (MQ=255)
              cATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCtgtg                                                                                                                 >  1:1262848/1‑126 (MQ=255)
                    gtgGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCagccagcc                                                                                              <  2:348528/139‑1 (MQ=255)
                           ttATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                       <  1:1527717/139‑1 (MQ=255)
                               ctcCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCaa                                                                                            <  2:139586/130‑1 (MQ=255)
                               ctcCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCaa                                                                                            >  1:139586/1‑130 (MQ=255)
                                        ggTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGAtt                                                                          >  1:62474/1‑139 (MQ=255)
                                         gTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCaa                                                                                                                         >  1:11373/1‑91 (MQ=255)
                                         gTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCaa                                                                                                                         <  2:11373/91‑1 (MQ=255)
                                              cGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTa                                                                                                                                 <  2:1657668/78‑1 (MQ=255)
                                              cGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCa                                                                    >  1:1673144/1‑139 (MQ=255)
                                              cGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCa                                                                    >  2:392267/1‑139 (MQ=255)
                                              cGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCa                                                                    >  2:339954/1‑139 (MQ=255)
                                               ggCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCagccagcc                                                                                              >  1:216758/1‑112 (MQ=255)
                                               ggCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCagccagcc                                                                                              <  2:216758/112‑1 (MQ=255)
                                                 cTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCAttt                                                                 <  2:1673144/139‑1 (MQ=255)
                                                   tttttGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                       >  1:781829/1‑115 (MQ=255)
                                                   tttttGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                       <  2:781829/115‑1 (MQ=255)
                                                                        tACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCt                                          >  1:1632624/1‑139 (MQ=255)
                                                                            ccTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGgcg                                      <  1:339954/139‑1 (MQ=255)
                                                                                      ggATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttcct                                                             <  2:1226638/106‑1 (MQ=255)
                                                                                      ggATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttcct                                                             >  1:1226638/1‑106 (MQ=255)
                                                                                       gATTACCGGTCTTTTACTACAGCCACGAAGGACATTaa                                                                                                                                <  1:1545063/38‑1 (MQ=255)
                                                                                       gATTACCGGTCTTTTACTACAGCCACGAAGGACATTaa                                                                                                                                >  2:1545063/1‑38 (MQ=255)
                                                                                       gATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                     <  1:1502234/81‑1 (MQ=255)
                                                                                       gATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                     >  2:1502234/1‑81 (MQ=255)
                                                                                                    ttACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGc                                                                                                      <  1:695791/51‑1 (MQ=255)
                                                                                                    ttACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGc                                                                                                      >  2:695791/1‑51 (MQ=255)
                                                                                                     tactacAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAgct             >  1:294010/1‑139 (MQ=255)
                                                                                                     tactacAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAgct             <  2:1632624/139‑1 (MQ=255)
                                                                                                          cAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAgct             <  1:749917/134‑1 (MQ=255)
                                                                                                          cAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAgct             >  2:749917/1‑134 (MQ=255)
                                                                                                            gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGc                                                                                                      <  2:737913/43‑1 (MQ=255)
                                                                                                            gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGc                                                                                                      >  1:737913/1‑43 (MQ=255)
                                                                                                            gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttccttcc                                                          <  1:1148764/87‑1 (MQ=255)
                                                                                                            gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttccttcc                                                          >  2:1148764/1‑87 (MQ=255)
                                                                                                            gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttccttc                                                           <  2:495084/86‑1 (MQ=255)
                                                                                                            gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttccttc                                                           >  1:495084/1‑86 (MQ=255)
                                                                                                            gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGgcg                                      >  1:342141/1‑107 (MQ=255)
                                                                                                            gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGgcg                                      <  2:342141/107‑1 (MQ=255)
                                                                                                            gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCa      >  2:407571/1‑139 (MQ=255)
                                                                                                            gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCa      >  1:496111/1‑139 (MQ=255)
                                                                                                            gCCACGAAGGACATTAAGGTGCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCtgctgcta      >  2:993405/1‑137 (MQ=255)
                                                                                                             ccACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGcagcca                                                                                                 <  2:1190532/47‑1 (MQ=255)
                                                                                                             ccACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGcagcca                                                                                                 >  1:1190532/1‑47 (MQ=255)
                                                                                                             ccACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCAttt                                                                 >  2:1332421/1‑79 (MQ=255)
                                                                                                             ccACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCAttt                                                                 <  1:1332421/79‑1 (MQ=255)
                                                                                                             ccACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAAc                                                <  2:1613653/96‑1 (MQ=255)
                                                                                                             ccACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAAc                                                >  1:1613653/1‑96 (MQ=255)
                                                                                                               aCGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAACCAGCCAACTGCCCAGCTTCTTGATTTgcg                                                                      >  1:687309/1‑72 (MQ=255)
                                                                                                               aCGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAACCAGCCAACTGCCCAGCTTCTTGATTTgcg                                                                      <  2:687309/72‑1 (MQ=255)
                                                                                                                cGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                     <  1:1497567/56‑1 (MQ=255)
                                                                                                                cGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                     >  2:1497567/1‑56 (MQ=255)
                                                                                                                cGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAAc  <  2:496111/139‑1 (MQ=255)
                                                                                                                         |                                                                                                                                 
ACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAAC  >  NC_000913/3819367‑3819617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: