Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,920,950	C→T	*80* (TAG→TAA)	atpE ←	F0 sector of membrane‑bound ATP synthase, subunit c

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,920,950	0	C	T	100.0%	251.8 / NA	76	*80* (TAG→TAA)	atpE	F0 sector of membrane‑bound ATP synthase, subunit c
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/36);  total (40/36)

CATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTGCGCCTTC  >  NC_000913/3920823‑3921081                                                                                                                                |                                                                                                                                    cATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGc                                                                                                                          >  2:954569/1‑139 (MQ=255)   tGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTAcg                                                                                                                                 <  1:625328/130‑1 (MQ=255)   tGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTAcg                                                                                                                                 >  2:625328/1‑130 (MQ=255)   tGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGa                                                                                                                        <  1:954569/139‑1 (MQ=255)         acagaacGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCa                                                                                                                  <  1:160169/139‑1 (MQ=255)                  acAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCttatta                                                                                                                                   <  1:1659003/113‑1 (MQ=255)                  acAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCttatta                                                                                                                                   >  2:1659003/1‑113 (MQ=255)                                    cctggccGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAca                                                                                                            >  1:1229117/1‑118 (MQ=255)                                    cctggccGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAca                                                                                                            <  2:1229117/118‑1 (MQ=255)                                        gccGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGatca                                                                                       <  1:174126/135‑1 (MQ=255)                                        gccGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGatca                                                                                       >  2:174126/1‑135 (MQ=255)                                             ggATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAg                                                                              >  1:1049951/1‑139 (MQ=255)                                              gATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGc                                                                             <  2:1437840/139‑1 (MQ=255)                                               aTTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCa                                                                            <  2:1049951/139‑1 (MQ=255)                                                  gttgCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGa                                                                                                                        >  1:760929/1‑92 (MQ=255)                                                  gttgCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGa                                                                                                                        <  2:760929/92‑1 (MQ=255)                                                   ttgCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGa                                                                                 >  2:987624/1‑130 (MQ=255)                                                   ttgCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGa                                                                                 <  1:987624/130‑1 (MQ=255)                                                     gCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacc                                                                      <  2:403901/139‑1 (MQ=255)                                                        ttAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCg                                                                                    <  2:1666430/122‑1 (MQ=255)                                                        ttAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCg                                                                                    >  1:1666430/1‑122 (MQ=255)                                                         tAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGAGATAGCATCCACCAGAc                                                                  >  1:1241282/1‑139 (MQ=255)                                                                cacaGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAcagacc                                                                                                        <  2:944703/94‑1 (MQ=255)                                                                cacaGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAcagacc                                                                                                        >  1:944703/1‑94 (MQ=255)                                                                  caGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGatcat                                                                                      >  2:960471/1‑110 (MQ=255)                                                                  caGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGatcat                                                                                      <  1:960471/110‑1 (MQ=255)                                                                   aGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGAGATAGCATCCACCAGACCCATAACGAt                                                        <  2:1241282/139‑1 (MQ=255)                                                                     cacaATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTAcgcg                                                                                                                               <  2:1230693/66‑1 (MQ=255)                                                                     cacaATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTAcgcg                                                                                                                               >  1:1230693/1‑66 (MQ=255)                                                                       caATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAg                                                    >  1:446951/1‑139 (MQ=255)                                                                          tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGa                                                                                 >  2:1630778/1‑107 (MQ=255)                                                                          tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGa                                                                                 <  1:1630778/107‑1 (MQ=255)                                                                                       ttAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCa                                                                            <  1:1480493/99‑1 (MQ=255)                                                                                       ttAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCa                                                                            >  2:1480493/1‑99 (MQ=255)                                                                                       ttAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACgcagca                                    >  1:259187/1‑139 (MQ=255)                                                                                         aaCGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCaga                                  <  2:259187/139‑1 (MQ=255)                                                                                          aCGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCagag                                 >  2:755684/1‑139 (MQ=255)                                                                                             ttCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTGCGCAGCAGAGGaa                              <  1:755684/139‑1 (MQ=255)                                                                                                    atTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCg                                                                                           <  1:505545/71‑1 (MQ=255)                                                                                                    atTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCg                                                                                           >  2:505545/1‑71 (MQ=255)                                                                                                     ttGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCa                                                                            >  2:81115/1‑85 (MQ=255)                                                                                                     ttGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCa                                                                            <  1:81115/85‑1 (MQ=255)                                                                                                       gCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACAt                                                                                                                    <  1:1450226/43‑1 (MQ=255)                                                                                                       gCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACAt                                                                                                                    >  2:1450226/1‑43 (MQ=255)                                                                                                        ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAcagacccag                                                                                                     <  2:200691/57‑1 (MQ=255)                                                                                                        ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAcagacccag                                                                                                     >  1:200691/1‑57 (MQ=255)                                                                                                        ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacca                                                                     <  2:835707/89‑1 (MQ=255)                                                                                                        ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacca                                                                     >  1:835707/1‑89 (MQ=255)                                                                                                        ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACt                                                <  1:873416/110‑1 (MQ=255)                                                                                                        ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACt                                                >  2:873416/1‑110 (MQ=255)                                                                                                        ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCaga                                  >  2:1259346/1‑124 (MQ=255)                                                                                                        ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCaga                                  <  1:1259346/124‑1 (MQ=255)                                                                                                        ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGt                   >  1:1365479/1‑139 (MQ=255)                                                                                                            ttAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACg                                         <  2:1059766/113‑1 (MQ=255)                                                                                                            ttAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACg                                         >  1:1059766/1‑113 (MQ=255)                                                                                                            ttAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGAc               >  1:1348159/1‑139 (MQ=255)                                                                                                             taaataaaAGCAACGCTTATTACGCGACAGCGAACATCACGTa                                                                                                              >  2:1281317/1‑43 (MQ=255)                                                                                                             taaataaaAGCAACGCTTATTACGCGACAGCGAACATCACGTa                                                                                                              <  1:1281317/43‑1 (MQ=255)                                                                                                             taaataaaAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCggg                                                                                  <  1:607752/71‑1 (MQ=255)                                                                                                             taaataaaAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCggg                                                                                  >  2:607752/1‑71 (MQ=255)                                                                                                             taaataaaAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCa                                                                            <  1:321664/77‑1 (MQ=255)                                                                                                             taaataaaAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCa                                                                            >  2:321664/1‑77 (MQ=255)                                                                                                             taaataaaAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGAcg              >  1:1617548/1‑139 (MQ=255)                                                                                                                ataaaAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACgcgc           >  1:1347959/1‑139 (MQ=255)                                                                                                                  aaaaGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCAGCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTg         >  2:1212344/1‑139 (MQ=255)                                                                                                                    aaGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTgcg       <  2:1348159/139‑1 (MQ=255)                                                                                                                     aGCAACGCTTATTACGCGACAGCGAACATCACGTa                                                                                                              <  2:116479/35‑1 (MQ=255)                                                                                                                     aGCAACGCTTATTACGCGACAGCGAACATCACGTa                                                                                                              >  1:116479/1‑35 (MQ=255)                                                                                                                     aGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacc                                                                      <  2:477076/75‑1 (MQ=255)                                                                                                                     aGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacc                                                                      >  1:477076/1‑75 (MQ=255)                                                                                                                     aGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTgcgc      >  1:883711/1‑139 (MQ=255)                                                                                                                         aCGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGatcat                                                                                      >  2:392851/1‑55 (MQ=255)                                                                                                                         aCGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGatcat                                                                                      <  1:392851/55‑1 (MQ=255)                                                                                                                         aCGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACg                                                          >  2:245413/1‑83 (MQ=255)                                                                                                                         aCGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACg                                                          <  1:245413/83‑1 (MQ=255)                                                                                                                         aCGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACg                                         >  1:175669/1‑100 (MQ=255)                                                                                                                         aCGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACg                                         <  2:175669/100‑1 (MQ=255)                                                                                                                         aCGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTGCGCCTTc  >  2:1610477/1‑139 (MQ=255)                                                                                                                                |                                                                                                                                    CATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTGCGCCTTC  >  NC_000913/3920823‑3921081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑