Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,047,494 A→G I177V (ATC→GTC)  polA → 5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,047,4940AG100.0% 339.9 / NA 94I177V (ATC→GTC) polA5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (52/42);  total (52/42)

CAGCACTGGCGATAAAGATATGGCGCAGCTGGTGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCATCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGC  >  NC_000913/4047367‑4047617
                                                                                                                               |                                                                                                                           
cAGCACTGGCGATAAAGATATGGCGCAGCTGGTGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTg                                                                                                                  <  1:547668/139‑1 (MQ=255)
        gCGATAAAGATATGGCGCAGCTGGTGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGAt                                                                                                          >  1:1254824/1‑139 (MQ=255)
               aGATATGGCGCAGCTGGTGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGAc                                                                                                   <  2:912831/139‑1 (MQ=255)
                  tatGGCGCAGCTGGTGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTcc                                                                                                                    >  1:596413/1‑119 (MQ=255)
                  tatGGCGCAGCTGGTGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTcc                                                                                                                    <  2:596413/119‑1 (MQ=255)
                     ggCGCAGCTGGTGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCctct                                                                                             >  2:545821/1‑139 (MQ=255)
                            cTGGTGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACa                                                                                      >  1:115067/1‑139 (MQ=255)
                               gTGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGActc                                                                                                 <  1:191419/125‑1 (MQ=255)
                               gTGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGActc                                                                                                 >  2:191419/1‑125 (MQ=255)
                                      cAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCt                                                                                                             <  1:1605167/106‑1 (MQ=255)
                                      cAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCt                                                                                                             >  2:1605167/1‑106 (MQ=255)
                                              aCGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGgc                                                                                                                <  2:1072653/95‑1 (MQ=255)
                                              aCGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGgc                                                                                                                >  1:1072653/1‑95 (MQ=255)
                                               cGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGActc                                                                                                 <  2:117093/109‑1 (MQ=255)
                                               cGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGActc                                                                                                 >  1:117093/1‑109 (MQ=255)
                                                    aTCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGa                                                                                                                         <  2:231254/80‑1 (MQ=255)
                                                    aTCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGa                                                                                                                         >  1:231254/1‑80 (MQ=255)
                                                    aTCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGgcg                                                                                                               >  1:811124/1‑90 (MQ=255)
                                                    aTCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGgcg                                                                                                               <  2:811124/90‑1 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGGGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTc                                                                  <  2:1013156/125‑1 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTcc                                                                                                                    <  2:448082/75‑1 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTcc                                                                                                                    >  1:448082/1‑75 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTgg                                                                                                                 <  2:422879/78‑1 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTgg                                                                                                                 >  1:422879/1‑78 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTg                                                                                                                  >  2:1431587/1‑77 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTg                                                                                                                  <  1:1431587/77‑1 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGgcgc                                                                                                              <  2:1150895/81‑1 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGgcgc                                                                                                              >  1:1150895/1‑81 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGt                                                                                                      >  2:1358124/1‑89 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGt                                                                                                      <  1:1358124/89‑1 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGActc                                                                                                 >  2:194888/1‑94 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGActc                                                                                                 <  1:194888/94‑1 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGAct                                                                                                  >  2:563301/1‑93 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGAct                                                                                                  <  1:563301/93‑1 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGAc                                                                                                   >  2:659990/1‑92 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGAc                                                                                                   <  1:659990/92‑1 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTc                                                                  >  1:1013156/1‑125 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGa                                                             >  2:964125/1‑130 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGa                                                             <  1:964125/130‑1 (MQ=255)
                                                              tGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACcgc                                                    >  2:1258914/1‑139 (MQ=255)
                                                               gACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACcgcg                                                   <  1:1258914/139‑1 (MQ=255)
                                                                   aaTACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTgg                                                                                                                 <  1:339520/73‑1 (MQ=255)
                                                                   aaTACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAgg                                               <  2:1254824/139‑1 (MQ=255)
                                                                   aaTACCATCCTCGGACCGGAAGAGGCGGTGAATAAGTACGGCGTGCCGCCAGAACTGAACGTCGATTTCCTgg                                                                                                                 >  2:339520/1‑73 (MQ=255)
                                                                     tACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATaa                                                                                        >  2:1445530/1‑96 (MQ=255)
                                                                     tACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATaa                                                                                        <  1:1445530/96‑1 (MQ=255)
                                                                     tACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCa                                             >  1:71935/1‑139 (MQ=255)
                                                                          tCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTcc                                                                                                                    <  2:1418260/63‑1 (MQ=255)
                                                                          tCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTcc                                                                                                                    >  1:1418260/1‑63 (MQ=255)
                                                                          tCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGa                                                                                                    >  1:1680195/1‑79 (MQ=255)
                                                                          tCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGa                                                                                                    <  2:1680195/79‑1 (MQ=255)
                                                                          tCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTgg                                                                               >  2:1654612/1‑100 (MQ=255)
                                                                          tCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTgg                                                                               <  1:1654612/100‑1 (MQ=255)
                                                                          tCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATtgct                                        >  1:1651345/1‑139 (MQ=255)
                                                                          tCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATtgct                                        >  2:41774/1‑139 (MQ=255)
                                                                               ggACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCaa                                    <  2:909533/138‑1 (MQ=255)
                                                                               ggACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCaa                                    >  1:909533/1‑138 (MQ=255)
                                                                                     gAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTg                             >  2:1172979/1‑139 (MQ=255)
                                                                                     gAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTg                             >  1:1439080/1‑139 (MQ=255)
                                                                                      aaGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTg                                                                                            >  2:1419308/1‑75 (MQ=255)
                                                                                      aaGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTg                                                                                            <  1:1419308/75‑1 (MQ=255)
                                                                                          ggtggtGAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGa                        <  1:1172979/139‑1 (MQ=255)
                                                                                           gtggtgAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGgcg                                                                                                               <  2:655219/51‑1 (MQ=255)
                                                                                           gtggtgAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGgcg                                                                                                               >  1:655219/1‑51 (MQ=255)
                                                                                           gtggtgAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGAct                                                                                                  >  2:260846/1‑64 (MQ=255)
                                                                                           gtggtgAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGAct                                                                                                  <  1:260846/64‑1 (MQ=255)
                                                                                           gtggtgAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGAc                       >  2:331259/1‑139 (MQ=255)
                                                                                           gtggtgAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGAc                       >  1:501018/1‑139 (MQ=255)
                                                                                             ggtgAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAAc                                                       >  1:1331177/1‑105 (MQ=255)
                                                                                             ggtgAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAAc                                                       <  2:1331177/105‑1 (MQ=255)
                                                                                              gtgAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTgg                    >  2:1384638/1‑139 (MQ=255)
                                                                                               tgAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATaa                                                                                        <  1:965617/70‑1 (MQ=255)
                                                                                               tgAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATaa                                                                                        >  2:965617/1‑70 (MQ=255)
                                                                                               tgAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGa                   >  2:569939/1‑139 (MQ=255)
                                                                                               tgAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGACGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGAc                       >  2:545119/1‑135 (MQ=255)
                                                                                               tgAATAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGACGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGAc                       <  1:545119/135‑1 (MQ=255)
                                                                                                 aaTAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATa                 <  1:545821/139‑1 (MQ=255)
                                                                                                   tAAGTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACg               <  2:1439080/139‑1 (MQ=255)
                                                                                                      gTACGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTg            >  2:117382/1‑139 (MQ=255)
                                                                                                        aCGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGgcg                                                                                                               >  1:141894/1‑38 (MQ=38)
                                                                                                        aCGGCGTGCCGCCAGAACTGATCGTCGATTTCCTGgcg                                                                                                               <  2:141894/38‑1 (MQ=38)
                                                                                                           gCGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGc       >  1:272896/1‑139 (MQ=255)
                                                                                                            cGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCggg                                                                      <  2:1473030/75‑1 (MQ=255)
                                                                                                            cGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCggg                                                                      >  1:1473030/1‑75 (MQ=255)
                                                                                                            cGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTc                                                                  <  2:1557896/79‑1 (MQ=255)
                                                                                                            cGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTc                                                                  >  1:1557896/1‑79 (MQ=255)
                                                                                                            cGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACTgcgc                                                  <  2:196089/95‑1 (MQ=255)
                                                                                                            cGTGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACTgcgc                                                  >  1:196089/1‑95 (MQ=255)
                                                                                                              tGCCGCCAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGa    >  1:360109/1‑139 (MQ=255)
                                                                                                               gccgccAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATACCATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCAtt                                           <  2:977496/99‑1 (MQ=255)
                                                                                                               gccgccAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCAtt                                           >  1:977496/1‑99 (MQ=255)
                                                                                                                ccgccAGAACTGATCGTCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGc  >  2:204353/1‑139 (MQ=255)
                                                                                                                 cgccAGAACTGATCGTCGATTTCCTGGCGCTGATggg                                                                                                       <  1:1360553/37‑1 (MQ=255)
                                                                                                                 cgccAGAACTGATCGTCGATTTCCTGGCGCTGATggg                                                                                                       >  2:1360553/1‑37 (MQ=255)
                                                                                                                               |                                                                                                                           
CAGCACTGGCGATAAAGATATGGCGCAGCTGGTGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCATCGATTTCCTGGCGCTGATGGGTGACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGC  >  NC_000913/4047367‑4047617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑