Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,061,157	G→A	*237* (TAG→TAA)	yihL →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,061,157	0	G	A	100.0%	174.6 / NA	54	*237* (TAG→TAA)	yihL	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/28);  total (26/28)

TATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAT  >  NC_000913/4061026‑4061281                                                                                                                                    |                                                                                                                             tATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGATAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGtttt                                                                                                                       >  2:1041885/1‑139 (MQ=255) tATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGtttt                                                                                                                       >  1:672442/1‑139 (MQ=255)   tCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTAt                                                                                                                     >  2:397586/1‑139 (MQ=255)       ggCGGTTCTGC‑GGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGa                                                                                                                >  1:524407/1‑139 (MQ=255)              cTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACaataa                                                                                                          >  1:746272/1‑139 (MQ=255)                      aTGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCa                                                                                                  >  1:528886/1‑139 (MQ=255)                        gCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaag                                                                                                <  1:1494327/139‑1 (MQ=255)                          cAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                              <  2:746272/139‑1 (MQ=255)                          cAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                              <  1:397586/139‑1 (MQ=255)                               aTCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaaa                                                                                             <  1:958264/135‑1 (MQ=255)                               aTCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaaa                                                                                             >  2:958264/1‑135 (MQ=255)                                   tAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTAc                                                                                     <  2:524407/139‑1 (MQ=255)                                              cATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                              <  1:918559/119‑1 (MQ=255)                                              cATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                              >  2:918559/1‑119 (MQ=255)                                               aTCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGaca                                                                         <  2:1192869/139‑1 (MQ=255)                                                   cgcCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATgg                                                                                                                   >  1:1578419/1‑93 (MQ=255)                                                   cgcCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATgg                                                                                                                   <  2:1578419/93‑1 (MQ=255)                                                   cgcCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                              <  2:1189674/114‑1 (MQ=255)                                                   cgcCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                              >  1:1189674/1‑114 (MQ=255)                                                       gACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCct                                                                 >  2:463112/1‑139 (MQ=255)                                                       gACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCct                                                                 <  2:672442/139‑1 (MQ=255)                                                                ggCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttattta                                                        <  2:528886/139‑1 (MQ=255)                                                                aggaatttttgccAAATCGATATTTAATAATGGCGATTTTAGGGCGTAAACCTTATCATTACTCTTTAAGTGTTTTATGGGGAAAAAAAAATACGGAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttattta                                                        <  1:1041885/128‑1 (MQ=255)                                                                  cAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTAt                                                                                                                     >  2:963022/1‑76 (MQ=255)                                                                  cAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTAt                                                                                                                     <  1:963022/76‑1 (MQ=255)                                                                  cAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAAGAACGc                                                                                                   >  1:1071859/1‑94 (MQ=255)                                                                  cAATATTGTCCAAATAAATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGc                                                                                                   <  2:1071859/94‑1 (MQ=255)                                                                          tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTAt                                                                                                                     >  1:31065/1‑68 (MQ=255)                                                                          tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTAt                                                                                                                     <  2:31065/68‑1 (MQ=255)                                                                          tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATg                                              >  1:1120900/1‑139 (MQ=255)                                                                           ccAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGc                                             >  2:286066/1‑139 (MQ=255)                                                                            cAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                              >  2:1021845/1‑89 (MQ=255)                                                                            cAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                              <  1:1021845/89‑1 (MQ=255)                                                                             aaaTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                              >  1:1571633/1‑88 (MQ=255)                                                                             aaaTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                              <  2:1571633/88‑1 (MQ=255)                                                                             aaaTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCt                                           <  1:286066/139‑1 (MQ=255)                                                                                         aaTATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAata                               <  2:1120900/139‑1 (MQ=255)                                                                                                   aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACa                                                                                                              >  2:965982/1‑50 (MQ=255)                                                                                                   aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACa                                                                                                              <  1:965982/50‑1 (MQ=255)                                                                                                   aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACaa                                                                                   >  2:427859/1‑77 (MQ=255)                                                                                                   aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACaa                                                                                   <  1:427859/77‑1 (MQ=255)                                                                                                   aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGa                     <  1:463112/139‑1 (MQ=255)                                                                                                   aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGa                     >  2:1553622/1‑139 (MQ=255)                                                                                                     ttGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACa                                                                                                              >  1:925685/1‑48 (MQ=255)                                                                                                     ttGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACa                                                                                                              <  2:925685/48‑1 (MQ=255)                                                                                                                     catcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaaa                                                                                             >  2:444849/1‑49 (MQ=255)                                                                                                                     catcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaaa                                                                                             <  1:444849/49‑1 (MQ=255)                                                                                                                     catcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACaa                                                                                   >  1:763374/1‑59 (MQ=255)                                                                                                                     catcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACaa                                                                                   <  2:763374/59‑1 (MQ=255)                                                                                                                     catcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACaa                                                                                   <  1:1141607/59‑1 (MQ=255)                                                                                                                     catcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACaa                                                                                   >  2:1141607/1‑59 (MQ=255)                                                                                                                     catcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCctc                                                                <  1:727994/78‑1 (MQ=255)                                                                                                                     catcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCctc                                                                >  2:727994/1‑78 (MQ=255)                                                                                                                      atcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAt  <  1:1553622/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                             TATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAT  >  NC_000913/4061026‑4061281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑