Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,077,469 G→A L7L (TTG→TTA yiiD → GNAT family putative N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,077,4690GA100.0% 219.6 / NA 68L7L (TTG→TTAyiiDGNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/31);  total (37/31)

TTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTGTCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACC  >  NC_000913/4077339‑4077588
                                                                                                                                  |                                                                                                                       
ttCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGa                                                                                                                 <  1:1319388/139‑1 (MQ=255)
   gtcgAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAAc                                                                                                              >  2:363438/1‑139 (MQ=255)
    tcgAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACa                                                                                                             >  2:1267927/1‑139 (MQ=255)
               cGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTAt                                                                                                  <  1:1267927/139‑1 (MQ=255)
                         agaTGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTAt                                                                                        <  1:1584236/139‑1 (MQ=255)
                             gAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCAcc                                                                                    >  2:538806/1‑139 (MQ=255)
                               acacGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCa                                                                                                                      >  2:595981/1‑103 (MQ=255)
                               acacGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCa                                                                                                                      <  1:595981/103‑1 (MQ=255)
                               acacGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                    <  2:389727/105‑1 (MQ=255)
                               acacGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                    >  1:389727/1‑105 (MQ=255)
                                   gCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCggg                                                                              >  2:757079/1‑139 (MQ=255)
                                   gCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCggg                                                                              >  1:1149005/1‑139 (MQ=255)
                                   gCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCggg                                                                              <  1:363438/139‑1 (MQ=255)
                                     aaaCAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGtt                                                                            >  2:1591668/1‑139 (MQ=255)
                                           gACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGAGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACaa                                                                      >  1:902249/1‑139 (MQ=255)
                                             cGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                    >  1:156596/1‑91 (MQ=255)
                                             cGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                    <  2:156596/91‑1 (MQ=255)
                                               cTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACag                                                                  >  1:359992/1‑139 (MQ=255)
                                                  cGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaag                                                               <  1:757079/139‑1 (MQ=255)
                                                  cGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaag                                                               <  2:1149005/139‑1 (MQ=255)
                                                   gCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaaga                                                              >  2:1186848/1‑139 (MQ=255)
                                                                aGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGt                                                 <  1:538806/139‑1 (MQ=255)
                                                                 ggTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaag                                                               >  1:84188/1‑124 (MQ=255)
                                                                 ggTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaag                                                               <  2:84188/124‑1 (MQ=255)
                                                                 ggTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGtt                                                >  1:566336/1‑139 (MQ=255)
                                                                 ggTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGtt                                                <  2:359992/139‑1 (MQ=255)
                                                                   tATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTAc                                              >  1:477143/1‑139 (MQ=255)
                                                                   tATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCACTATGTATCACCTTCGGGTTCCACaaa                                                                     <  2:831246/116‑1 (MQ=255)
                                                                   tATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCACTATGTATCACCTTCGGGTTCCACaaa                                                                     >  1:831246/1‑116 (MQ=255)
                                                                    aTCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCa                                                                                                                      <  2:819433/66‑1 (MQ=255)
                                                                    aTCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCa                                                                                                                      >  1:819433/1‑66 (MQ=255)
                                                                    aTCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACggg                                                                                                                  <  2:480305/70‑1 (MQ=255)
                                                                    aTCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACggg                                                                                                                  >  1:480305/1‑70 (MQ=255)
                                                                    aTCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGa                                                                                                                 <  1:1188847/71‑1 (MQ=255)
                                                                    aTCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGa                                                                                                                 >  2:1188847/1‑71 (MQ=255)
                                                                    aTCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACt                                             >  2:577347/1‑139 (MQ=255)
                                                                                tgatgGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCt                                 >  1:173268/1‑139 (MQ=255)
                                                                                     gCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACaaa                                                                     <  1:1057618/98‑1 (MQ=255)
                                                                                     gCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGaa                            >  1:369688/1‑139 (MQ=255)
                                                                                     gCCCCGCGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACaaa                                                                     >  2:1057618/1‑98 (MQ=255)
                                                                                      ccccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGaaa                           >  2:1066146/1‑139 (MQ=255)
                                                                                           tGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTg                      >  2:1447272/1‑139 (MQ=255)
                                                                                               aTTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTa                  <  2:566336/139‑1 (MQ=255)
                                                                                               aTTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTa                  <  1:1066146/139‑1 (MQ=255)
                                                                                               aTTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTa                  <  1:1186848/139‑1 (MQ=255)
                                                                                                 tCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGaaa                                                                                                               <  1:634865/44‑1 (MQ=255)
                                                                                                 tCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGaaa                                                                                                               >  2:634865/1‑44 (MQ=255)
                                                                                                    ggTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGccc             <  2:173268/139‑1 (MQ=255)
                                                                                                     gTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAagaga                                                                                                        <  1:784918/47‑1 (MQ=255)
                                                                                                     gTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAagaga                                                                                                        >  2:784918/1‑47 (MQ=255)
                                                                                                     gTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGcccc            >  2:514707/1‑139 (MQ=255)
                                                                                                       tGCAGGTATGAGCCAGCTTCCAGGGTTATCACggg                                                                                                                  <  1:895607/35‑1 (MQ=255)
                                                                                                       tGCAGGTATGAGCCAGCTTCCAGGGTTATCACggg                                                                                                                  >  2:895607/1‑35 (MQ=37)
                                                                                                       tGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaagaa                                                             <  2:1589507/88‑1 (MQ=255)
                                                                                                       tGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaagaa                                                             >  1:1589507/1‑88 (MQ=255)
                                                                                                        gCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTcc                                                                          >  1:805109/1‑74 (MQ=255)
                                                                                                        gCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTcc                                                                          <  2:805109/74‑1 (MQ=255)
                                                                                                         cAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAagagagag                                                                                                     >  2:1363445/1‑46 (MQ=255)
                                                                                                         cAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAagagagag                                                                                                     <  1:1363445/46‑1 (MQ=255)
                                                                                                               tGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAAcc  >  1:1366923/1‑139 (MQ=255)
                                                                                                                      gCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTAt                                                                                        >  1:565611/1‑46 (MQ=255)
                                                                                                                      gCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTAt                                                                                        <  2:565611/46‑1 (MQ=255)
                                                                                                                      gCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTa                                                       <  2:566897/79‑1 (MQ=255)
                                                                                                                      gCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTa                                                       >  1:566897/1‑79 (MQ=255)
                                                                                                                       cTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATc                                                                                                 <  1:1490345/36‑1 (MQ=255)
                                                                                                                       cTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATc                                                                                                 >  2:1490345/1‑36 (MQ=255)
                                                                                                                         tCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTa                  <  1:1452043/113‑1 (MQ=255)
                                                                                                                         tCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTa                  >  2:1452043/1‑113 (MQ=255)
                                                                                                                                  |                                                                                                                       
TTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTGTCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACC  >  NC_000913/4077339‑4077588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: