Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,331,634 C→T A378V (GCG→GTG)  proP → proline/glycine betaine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,331,6340CT100.0% 234.8 / NA 72A378V (GCG→GTG) proPproline/glycine betaine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/34);  total (38/34)

TTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATT  >  NC_000913/4331499‑4331768
                                                                                                                                       |                                                                                                                                      
ttGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGAt                                                                                                                                     >  2:876785/1‑139 (MQ=255)
 tGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGCCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGAta                                                                                                                                    >  1:389668/1‑138 (MQ=255)
   cccTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGtt                                                                                                                                  >  2:2230/1‑139 (MQ=255)
     cTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGTTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATATTcc                                                                                                                                <  2:389668/139‑1 (MQ=255)
     cTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTcc                                                                                                                                >  1:714271/1‑139 (MQ=255)
           gtgtTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                          <  1:787378/139‑1 (MQ=255)
            tgtTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCa                                                                                                                         <  1:1403139/139‑1 (MQ=255)
             gtTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTccc                                                                                                                               <  2:832521/132‑1 (MQ=255)
             gtTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTccc                                                                                                                               >  1:832521/1‑132 (MQ=255)
                  cGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCg                                                                                                                   >  2:844182/1‑139 (MQ=255)
                   gATCCCGGGGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTTATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGt                                                                                                                  <  2:1036381/139‑1 (MQ=255)
                      cccGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTAc                                                                                                               <  1:167300/139‑1 (MQ=255)
                                           cAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCAttt                                                                                          >  1:1355179/1‑139 (MQ=255)
                                              tAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAat                                                                                       >  1:1442503/1‑139 (MQ=255)
                                                aCGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAatat                                                                                     >  2:76106/1‑139 (MQ=255)
                                                  gTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATAttt                                                                                   <  1:1493197/139‑1 (MQ=255)
                                                      tCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCat                                                                                                                        <  1:1105722/98‑1 (MQ=255)
                                                      tCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCat                                                                                                                        >  2:1105722/1‑98 (MQ=255)
                                                             gATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCa                                                                                                                         >  1:590419/1‑90 (MQ=255)
                                                             gATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCa                                                                                                                         <  2:590419/90‑1 (MQ=255)
                                                                    gCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTc                                                                 <  1:876785/139‑1 (MQ=255)
                                                                                  gCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTg                                                   <  2:1442503/139‑1 (MQ=255)
                                                                                   cTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTgg                                                  >  2:1529737/1‑139 (MQ=255)
                                                                                       cGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTggcggc                                              >  2:409524/1‑139 (MQ=255)
                                                                                            aTCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAgcgcg                                                                                                         <  1:427688/75‑1 (MQ=255)
                                                                                            aTCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAgcgcg                                                                                                         >  2:427688/1‑75 (MQ=255)
                                                                                                   aCTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                  >  1:386398/1‑75 (MQ=255)
                                                                                                   aCTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                  <  2:386398/75‑1 (MQ=255)
                                                                                                       cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAg                                                                                                             <  1:800484/60‑1 (MQ=255)
                                                                                                       cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAg                                                                                                             >  2:800484/1‑60 (MQ=255)
                                                                                                       cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCAtt                                                                                           >  2:1364456/1‑78 (MQ=255)
                                                                                                       cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCAtt                                                                                           <  1:1364456/78‑1 (MQ=255)
                                                                                                       cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGc                              >  1:98447/1‑139 (MQ=255)
                                                                                                        tttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                  >  1:462248/1‑70 (MQ=255)
                                                                                                        tttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                  <  2:462248/70‑1 (MQ=255)
                                                                                                        tttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCt                             >  2:79019/1‑139 (MQ=255)
                                                                                                         ttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTc                            >  1:846826/1‑139 (MQ=255)
                                                                                                            cGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCa                         <  2:1633676/139‑1 (MQ=255)
                                                                                                            cGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCa                         >  2:1641168/1‑139 (MQ=255)
                                                                                                              ggCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGa                       <  1:2230/139‑1 (MQ=255)
                                                                                                              ggCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGa                       <  1:76106/139‑1 (MQ=255)
                                                                                                               gCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCat                                                                                                                        <  1:261011/41‑1 (MQ=255)
                                                                                                               gCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCat                                                                                                                        >  2:261011/1‑41 (MQ=255)
                                                                                                                cGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAAt                     <  1:79019/139‑1 (MQ=255)
                                                                                                                 gTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATc                    >  2:1520570/1‑139 (MQ=255)
                                                                                                                  ttATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                                >  1:1042796/1‑62 (MQ=255)
                                                                                                                  ttATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCt                   >  1:985669/1‑139 (MQ=255)
                                                                                                                  ttATGGCTTCTACCTTGCCACTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                                <  2:1042796/62‑1 (MQ=255)
                                                                                                                    aTGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                  >  2:463390/1‑58 (MQ=255)
                                                                                                                    aTGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                  <  1:463390/58‑1 (MQ=255)
                                                                                                                     tGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCtgat                <  1:1520570/139‑1 (MQ=255)
                                                                                                                         ttCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCctgg                                          >  1:724383/1‑109 (MQ=255)
                                                                                                                         ttCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCctgg                                          <  2:724383/109‑1 (MQ=255)
                                                                                                                         ttCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGatgatg            >  2:1072463/1‑139 (MQ=255)
                                                                                                                          tCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCt                                                    <  2:1653189/98‑1 (MQ=255)
                                                                                                                          tCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCt                                                    >  1:1653189/1‑98 (MQ=255)
                                                                                                                          tCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAACTCGCAGAATCTGATGAtgc           <  1:1529737/139‑1 (MQ=255)
                                                                                                                           cTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                  >  1:529695/1‑51 (MQ=255)
                                                                                                                           cTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                  <  2:529695/51‑1 (MQ=255)
                                                                                                                            tACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcc          >  2:914577/1‑138 (MQ=255)
                                                                                                                            tACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcc          <  1:914577/138‑1 (MQ=255)
                                                                                                                             aCCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcctg        <  1:1641168/139‑1 (MQ=255)
                                                                                                                             aCCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcctg        >  2:1059936/1‑139 (MQ=255)
                                                                                                                               cTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                  <  2:645070/47‑1 (MQ=255)
                                                                                                                               cTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                  >  1:645070/1‑47 (MQ=255)
                                                                                                                                 tGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                                <  1:140334/47‑1 (MQ=255)
                                                                                                                                 tGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                                >  2:140334/1‑47 (MQ=255)
                                                                                                                                  gCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcggc                                                                                              <  1:902769/48‑1 (MQ=255)
                                                                                                                                  gCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcggc                                                                                              >  2:902769/1‑48 (MQ=255)
                                                                                                                                  gCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAt   >  2:117372/1‑139 (MQ=255)
                                                                                                                                  gCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAt   <  2:1355179/139‑1 (MQ=255)
                                                                                                                                   ccAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAtt  >  2:65280/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATT  >  NC_000913/4331499‑4331768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑