Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,353,081	G→A	*58* (TAG→TAA)	ghoT →	toxin of GhoTS toxin‑antitoxin pair, membrane‑lytic protein, stimulator of persister cell formation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,353,081	0	G	A	100.0%	231.5 / NA	71	*58* (TAG→TAA)	ghoT	toxin of GhoTS toxin‑antitoxin pair, membrane‑lytic protein, stimulator of persister cell formation
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/36);  total (35/36)

ATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCT  >  NC_000913/4352958‑4353207                                                                                                                            |                                                                                                                               atCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAagag                                                                                                                             >  2:172680/1‑127 (MQ=255) atCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAagag                                                                                                                             <  1:172680/127‑1 (MQ=255) atCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGt                                                                                                                 <  2:583577/139‑1 (MQ=255) atCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGt                                                                                                                 >  2:926271/1‑139 (MQ=255) atCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGt                                                                                                                 >  2:247333/1‑139 (MQ=255)      ttGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGAcc                                                                                                            >  1:302263/1‑139 (MQ=255)      ttGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGAc                                                                                                             <  1:1334862/138‑1 (MQ=255)      ttGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGAc                                                                                                             >  2:1334862/1‑138 (MQ=255)         tCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGAccc                                                                                                           >  1:839005/1‑137 (MQ=255)         tCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGAccc                                                                                                           <  2:839005/137‑1 (MQ=255)           aTTATCTGGTTTATCTCACATGAGGAAACACATATTCGTTAACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGAc                                                                                                             >  1:1032999/1‑133 (MQ=255)           aTTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGAc                                                                                                             <  2:1032999/133‑1 (MQ=255)             tATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGt                                                                                                     <  1:926271/139‑1 (MQ=255)                   gTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTa                                                                                                   <  1:1543820/135‑1 (MQ=255)                   gTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTa                                                                                                   >  2:1543820/1‑135 (MQ=255)                   gTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGt                                                                                               >  1:865823/1‑139 (MQ=255)                    tttATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTg                                                                                              >  1:867227/1‑139 (MQ=255)                         ctcACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAg                                                                                                                       <  2:484518/109‑1 (MQ=255)                         ctcACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAg                                                                                                                       >  1:484518/1‑109 (MQ=255)                          tcACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGa                                                                                        <  1:247333/139‑1 (MQ=255)                          tcACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGa                                                                                        <  2:865823/139‑1 (MQ=255)                             caTGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGt                                                                                                     <  1:951354/123‑1 (MQ=255)                             caTGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGt                                                                                                     >  2:951354/1‑123 (MQ=255)                               tGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGt                                                                                   <  2:867227/139‑1 (MQ=255)                                 agaAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGtct                                                                                 >  1:1091239/1‑139 (MQ=255)                                   aaaaCACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGAccc                                                                                                           >  2:418176/1‑111 (MQ=255)                                   aaaaCACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGAccc                                                                                                           <  1:418176/111‑1 (MQ=255)                                      acacATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTctcct                                                                            <  1:407129/139‑1 (MQ=255)                                            atTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAg                                                                                      <  1:1455091/123‑1 (MQ=255)                                            atTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAg                                                                                      >  2:1455091/1‑123 (MQ=255)                                                 tttACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGc                                                                                                         <  1:627461/99‑1 (MQ=255)                                                 tttACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGc                                                                                                         >  2:627461/1‑99 (MQ=255)                                                              ttCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTgg                                                                                                      <  1:758512/89‑1 (MQ=255)                                                              ttCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTgg                                                                                                      >  2:758512/1‑89 (MQ=255)                                                                    gTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTa                                                                                                                        <  1:477996/65‑1 (MQ=255)                                                                    gTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTa                                                                                                                        >  2:477996/1‑65 (MQ=255)                                                                    gTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACg                                                       <  2:20043/130‑1 (MQ=255)                                                                    gTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACg                                                       >  1:20043/1‑130 (MQ=255)                                                                         aaTAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGt                                         >  2:379999/1‑139 (MQ=255)                                                                              ccTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTg                                                                                              <  1:195798/81‑1 (MQ=255)                                                                              ccTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTg                                                                                              >  2:195798/1‑81 (MQ=255)                                                                                tGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGa                                  <  1:379999/139‑1 (MQ=255)                                                                                tGGCCAATGAGTCTGCCTGTGGCATTACTATTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGa                                  <  2:1091239/139‑1 (MQ=255)                                                                                   ccAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGAccc                                                                                                           >  1:880771/1‑63 (MQ=255)                                                                                   ccAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGAccc                                                                                                           <  2:880771/63‑1 (MQ=255)                                                                                   ccAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTctcct                                                                            >  2:1150569/1‑94 (MQ=255)                                                                                   ccAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTctcct                                                                            <  1:1150569/94‑1 (MQ=255)                                                                                   ccAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCa                                           <  1:72258/127‑1 (MQ=255)                                                                                   ccAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCa                                           >  2:72258/1‑127 (MQ=255)                                                                                       tGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTctcctc                                                                           >  2:1011085/1‑91 (MQ=255)                                                                                       tGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTctcctc                                                                           <  1:1011085/91‑1 (MQ=255)                                                                                       tGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTctcct                                                                            >  2:993450/1‑90 (MQ=255)                                                                                       tGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTctcct                                                                            <  1:993450/90‑1 (MQ=255)                                                                                             tGCCTGTGGCATTACTTTTTTCTCTCTTTTTAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGAt                                                     <  2:1212731/107‑1 (MQ=255)                                                                                             tGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGaa                                                                                            >  2:981952/1‑68 (MQ=255)                                                                                             tGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGaa                                                                                            <  1:981952/68‑1 (MQ=255)                                                                                             tGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGAt                                                     >  1:1212731/1‑107 (MQ=255)                                                                                             tGCCTGTGGCATTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCAt                     >  2:882515/1‑139 (MQ=255)                                                                                                       aTTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAg                                          >  2:1082477/1‑108 (MQ=255)                                                                                                       aTTACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAg                                          <  1:1082477/108‑1 (MQ=255)                                                                                                        ttACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTgg                                                                                                               <  2:95193/38‑1 (MQ=255)                                                                                                        ttACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTgg                                                                                                               >  1:95193/1‑38 (MQ=255)                                                                                                         tACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTa                                                                                                   <  2:448114/49‑1 (MQ=255)                                                                                                         tACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTa                                                                                                   >  1:448114/1‑49 (MQ=255)                                                                                                         tACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCt                                    <  1:804468/112‑1 (MQ=255)                                                                                                         tACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCt                                    >  2:804468/1‑112 (MQ=255)                                                                                                         tACTTTTTTCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAatt         >  1:398926/1‑139 (MQ=255)                                                                                                               tttCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTc   <  2:302263/139‑1 (MQ=255)                                                                                                                ttCTCTCTTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCt  <  2:398926/139‑1 (MQ=255)                                                                                                                   tctctTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTa                   <  1:1284753/119‑1 (MQ=255)                                                                                                                   tctctTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTa                   >  2:1284753/1‑119 (MQ=255)                                                                                                                     tctTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGtct                                                                                 >  1:1203384/1‑55 (MQ=255)                                                                                                                     tctTTTAAGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGtct                                                                                 <  2:1203384/55‑1 (MQ=255)                                                                                                                            |                                                                                                                               ATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCT  >  NC_000913/4352958‑4353207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑