Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,389,934	A→G	Y143H (TAC→CAC)	psd ←	phosphatidylserine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,389,934	0	A	G	100.0%	199.1 / NA	56	Y143H (TAC→CAC)	psd	phosphatidylserine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/28);  total (28/28)

AGGCAAATCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTAGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTT  >  NC_000913/4389798‑4390062                                                                                                                                         |                                                                                                                                 aGGCAAATCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCggtggtggt                                                                                                                                <  1:1174903/139‑1 (MQ=255)     aaaTCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcg                                                                                                                            <  1:1678852/139‑1 (MQ=255)       aTCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAcgcg                                                                                                                          >  1:827894/1‑139 (MQ=255)       aTCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAc                                                                                                                             >  2:862329/1‑136 (MQ=255)       aTCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCAc                                                                                                                             <  1:862329/136‑1 (MQ=255)        tCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCgg                                                                                                                         <  2:1118376/139‑1 (MQ=255)         cACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCggg                                                                                                                        <  1:438671/139‑1 (MQ=255)             cgttcgttACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGgaga                                                                                                                    <  2:827894/139‑1 (MQ=255)                            aaCAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCATGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACaa                                                                                                     >  1:547681/1‑139 (MQ=255)                                 aTTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTa                                                                                                >  2:1360740/1‑139 (MQ=255)                                  ttCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCATGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTAc                                                                                               <  2:547681/139‑1 (MQ=255)                                       cACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTg                                                                                          >  1:1085771/1‑139 (MQ=255)                                       cACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAAATCATCTCACGCAGAATACCGTTGCACGGCATATGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTca                                                                                                        <  2:1518504/125‑1 (MQ=255)                                       cACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAAATCATCTCACGCAGAATACCGTTGCACGGCATATGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTca                                                                                                        >  1:1518504/1‑125 (MQ=255)                                                     tGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGt                                                                                                          >  1:1184824/1‑109 (MQ=255)                                                     tGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGt                                                                                                          <  2:1184824/109‑1 (MQ=255)                                                     tGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGc                                                                            >  1:1037191/1‑139 (MQ=255)                                                     tGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGc                                                                            >  1:69236/1‑139 (MQ=255)                                                       agaTGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCa                                                                          <  1:1360740/139‑1 (MQ=255)                                                                      aagaGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGc                                                                                         >  1:1198840/1‑109 (MQ=255)                                                                      aagaGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTAGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGc                                                                                         <  2:1198840/109‑1 (MQ=255)                                                                           aTCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCcagc                                                      >  2:853858/1‑139 (MQ=255)                                                                            tCGCCTGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCcagca                                                     >  2:1352359/1‑139 (MQ=255)                                                                              gCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCggtggtggt                                                                                                                                <  1:252132/62‑1 (MQ=255)                                                                              gCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCggtggtggt                                                                                                                                >  2:252132/1‑62 (MQ=255)                                                                                   gCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGTGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTc                                              <  2:1085771/139‑1 (MQ=255)                                                                                   gCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAg                                                                                                              <  1:720622/75‑1 (MQ=255)                                                                                   gCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAg                                                                                                              >  2:720622/1‑75 (MQ=255)                                                                                   gCACGTAGACCATCTCACGCAGAATACCGTTGAACGGCATGAGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTGACAAACGTAACGTTGCGGACCAGGTCCGCCATCAGCTAGTTGCCTGCCCGCAGGCCTTc                                              >  2:1185615/1‑139 (MQ=255)                                                                                    cACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCg                                             <  1:1352359/139‑1 (MQ=255)                                                                                       gTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCgg                                                                                                                         <  1:1556696/60‑1 (MQ=255)                                                                                       gTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCgg                                                                                                                         >  2:1556696/1‑60 (MQ=255)                                                                                         aGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCt                                        <  2:1037191/139‑1 (MQ=255)                                                                                                ctcACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGt                                                                                                             >  2:1343342/1‑63 (MQ=255)                                                                                                ctcACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGt                                                                                                             <  1:1343342/63‑1 (MQ=255)                                                                                                 tcACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTtgt                                <  1:853858/139‑1 (MQ=255)                                                                                                  cACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTtgtg                               >  2:1562637/1‑139 (MQ=255)                                                                                                     gCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCg                                             <  1:1291988/122‑1 (MQ=255)                                                                                                     gCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCg                                             >  2:1291988/1‑122 (MQ=255)                                                                                                     gCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGcc                            >  1:1081121/1‑139 (MQ=255)                                                                                                         aaTACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTg                        <  1:1562637/139‑1 (MQ=255)                                                                                                           tACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTAc                                                                                               <  2:825500/66‑1 (MQ=255)                                                                                                           tACCGTTGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTAc                                                                                               >  1:825500/1‑66 (MQ=255)                                                                                                                ttGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTcac                                                                                                       >  1:1215256/1‑53 (MQ=255)                                                                                                                ttGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTcac                                                                                                       <  2:1215256/53‑1 (MQ=255)                                                                                                                 tGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCg                                                                             <  1:1494064/78‑1 (MQ=255)                                                                                                                 tGCACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCg                                                                             >  2:1494064/1‑78 (MQ=255)                                                                                                                   cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGa                                            >  2:1083850/1‑109 (MQ=255)                                                                                                                   cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGa                                            <  1:1083850/109‑1 (MQ=255)                                                                                                                   cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCa                 <  1:449703/136‑1 (MQ=255)                                                                                                                   cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCa                 >  2:449703/1‑136 (MQ=255)                                                                                                                   cACGGCATGTGTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGa              >  2:568750/1‑139 (MQ=255)                                                                                                                          tgtgTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGt                                                                                >  1:655247/1‑66 (MQ=255)                                                                                                                          tgtgTACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGt                                                                                <  2:655247/66‑1 (MQ=255)                                                                                                                           gtgtACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTCCCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCcagcag                                                    <  2:1199392/93‑1 (MQ=255)                                                                                                                           gtgtACGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCcagcag                                                    >  1:1199392/1‑93 (MQ=255)                                                                                                                               aCGCGGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATcttctt  <  2:1081121/139‑1 (MQ=255)                                                                                                                                cgcgGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTg                                                                                          <  2:1144950/50‑1 (MQ=255)                                                                                                                                cgcgGTGGTGGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTg                                                                                          >  1:1144950/1‑50 (MQ=255)                                                                                                                                      ggtggtCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTc                                                                               <  2:204930/55‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 AGGCAAATCACGCGTTCGTTACGGGCAAACAGATTCGGCACGTTCTGAGCCGTGAGATGGTTAACGGAGAAGAGATCGCCCGGCACGTAGATCATCTCACGCAGAATACCGTTGCACGGCATGTGTACGCGGTGGTAGTCACGCGGGGAGAGGTAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTT  >  NC_000913/4389798‑4390062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑