| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,515,284 | C→T | G465D (GGC→GAC) | fecA ← | TonB‑dependent outer membrane ferric citrate transporter and signal transducer, ferric citrate extracelluar receptor, FecR‑interacting protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,515,284 | 0 | C | T | 96.7% | 192.5 / ‑1.0 | 61 | coding (1394/2325 nt) | fecA | TonB‑dependent outer membrane ferric citrate transporter and signal transducer, ferric citrate extracelluar receptor, FecR‑interacting protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (29/30); minor base . (1/1); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01 | |||||||||||
GACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCG > NC_000913/4515161‑4515411 | gACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCATGAGTCGGACGGCAACAGcc < 2:1111217/139‑1 (MQ=255) aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGccc > 1:1101363/1‑139 (MQ=255) aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGccc > 1:415789/1‑139 (MQ=255) aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGccc > 2:566215/1‑139 (MQ=255) aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGccc > 2:764159/1‑139 (MQ=255) aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTACCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAG‑TGGACGGCAACTGCCCg > 1:166518/1‑139 (MQ=255) cTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCg < 1:1610537/139‑1 (MQ=255) atatGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGcc < 1:914608/133‑1 (MQ=255) atatGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGcc > 2:914608/1‑133 (MQ=255) tCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg < 1:84157/139‑1 (MQ=255) cGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCAtt > 1:402461/1‑139 (MQ=255) tGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTc < 1:764159/139‑1 (MQ=255) tGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTc < 2:1226099/139‑1 (MQ=255) aTGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTgtag < 1:1342188/126‑1 (MQ=255) aTGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTgtag > 2:1342188/1‑126 (MQ=255) ggTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGc > 2:1627160/1‑139 (MQ=255) aGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTACGCCTCGGTGCCGGAACGCGTATCGCCGTCGTAAGGGCTTGAGTCGGACGGCAACTGCTCGCTGCTGGTGGCGGTGTAGTAACGGATTTCATGCGTTGa < 1:1029345/139‑1 (MQ=255) gCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCgg > 1:315952/1‑110 (MQ=255) gCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCgg < 2:315952/110‑1 (MQ=255) ccGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGattcat < 1:1645378/139‑1 (MQ=255) gATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGattcaatc > 2:631210/1‑136 (MQ=255) ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCgg > 2:1366319/1‑53 (MQ=255) ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCgg < 1:1366319/53‑1 (MQ=255) ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCg > 2:917331/1‑101 (MQ=255) ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCg < 1:917331/101‑1 (MQ=255) ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCg > 2:1174608/1‑139 (MQ=255) gCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCg < 2:427610/100‑1 (MQ=255) gCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCg > 1:427610/1‑100 (MQ=255) tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGcc < 1:1523390/61‑1 (MQ=255) tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGcc > 2:1523390/1‑61 (MQ=255) tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg > 1:1405392/1‑73 (MQ=255) tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg < 2:1405392/73‑1 (MQ=255) tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCg < 2:1189603/135‑1 (MQ=255) tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCg > 1:1189603/1‑135 (MQ=255) tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTgcg > 2:1569374/1‑139 (MQ=255) tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTgcg > 1:94082/1‑139 (MQ=255) cgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg > 1:879658/1‑71 (MQ=255) cgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg < 2:879658/71‑1 (MQ=255) cgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTCTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGcgcg < 2:1101363/139‑1 (MQ=255) ccTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGa < 2:94082/139‑1 (MQ=255) gCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCg < 1:1174608/139‑1 (MQ=255) ggAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTgta < 1:1583207/66‑1 (MQ=255) ggAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTgta > 2:1583207/1‑66 (MQ=255) aCGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATa < 1:1627160/139‑1 (MQ=255) cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg < 2:1223130/53‑1 (MQ=255) cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg > 1:1223130/1‑53 (MQ=255) cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCg < 1:1084197/55‑1 (MQ=255) cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCg > 2:1084197/1‑55 (MQ=255) cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCaaa < 1:724948/91‑1 (MQ=255) cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCaaa > 2:724948/1‑91 (MQ=255) cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGc < 2:557786/128‑1 (MQ=255) cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGc > 1:557786/1‑128 (MQ=255) cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAg > 1:334438/1‑139 (MQ=255) tATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGAt > 1:1321596/1‑139 (MQ=255) aTCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg > 1:993261/1‑50 (MQ=255) aTCGCGGTCGTAAGGGCTTGAGTCGGACGCCAACTGCCCGCTGCtggtgg < 2:993261/50‑1 (MQ=255) tAAGGGCTTGAG‑TGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCg < 2:166518/139‑1 (MQ=255) aaGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCg < 2:402461/139‑1 (MQ=255) aaGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCAATCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCg < 1:631210/139‑1 (MQ=255) ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCaaa > 2:39277/1‑71 (MQ=255) ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCaaa < 1:39277/71‑1 (MQ=255) | GACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCG > NC_000913/4515161‑4515411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |