| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,604,202 | G→A | *242* (TAG→TAA) E15K (GAA→AAA) |
yjjQ → bglJ → |
putative transcriptional regulator bgl operon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,604,202 | 0 | G | A | 96.8% | 200.3 / ‑1.1 | 63 | coding (726/726 nt) coding (43/678 nt) | yjjQ bglJ | putative transcriptional regulator bgl operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (30/31); minor base . (1/1); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
CGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCT > NC_000913/4604079‑4604322 | cGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGa > 2:910250/1‑139 (MQ=255)cGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGa > 1:600282/1‑139 (MQ=255)cGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGa < 2:412106/139‑1 (MQ=255) cTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATg < 2:1582319/136‑1 (MQ=255) cTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATg > 1:1582319/1‑136 (MQ=255) cTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGagt < 1:1175915/139‑1 (MQ=255) tGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACGTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATc < 2:126460/139‑1 (MQ=255) tATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGCGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCAt < 2:989832/127‑1 (MQ=255) tATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCAt > 1:989832/1‑127 (MQ=255) gATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAAt < 2:473056/112‑1 (MQ=255) gATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAAt > 1:473056/1‑112 (MQ=255) cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAAt > 1:293327/1‑101 (MQ=255) cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAAt < 2:293327/101‑1 (MQ=255) cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAgtagta < 2:58359/116‑1 (MQ=255) cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAgtagta > 1:58359/1‑116 (MQ=255) cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATA‑AAAAATGCGTCATGAGTAGTATCg > 1:272175/1‑118 (MQ=255) cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATA‑AAAAATGCGTCATGAGTAGTATCg < 2:272175/118‑1 (MQ=255) tGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCa < 1:1465895/100‑1 (MQ=255) tGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCa > 2:1465895/1‑100 (MQ=255) gagaACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGt < 1:910250/139‑1 (MQ=255) aacttaacttGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGt > 2:44403/1‑136 (MQ=255) aacttaacttGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGt < 1:44403/136‑1 (MQ=255) cttaacttGCCTTCTGTTTATGGAGATGCCGCAGAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGag < 1:955338/96‑1 (MQ=255) cttaacttGCCTTCTGTTTATGGAGATGCCGCAGAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGag > 2:955338/1‑96 (MQ=255) cttaacttGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGag < 1:770216/94‑1 (MQ=255) cttaacttGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGag > 2:770216/1‑94 (MQ=255) cttaacttGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGa > 2:1525072/1‑93 (MQ=255) cttaacttGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGa < 1:1525072/93‑1 (MQ=255) cttaacttGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGa > 1:805991/1‑122 (MQ=255) cttaacttGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGa < 2:805991/122‑1 (MQ=255) cttaacttGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAAccc > 2:848505/1‑139 (MQ=255) cttaacttGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAAccc > 2:108873/1‑139 (MQ=255) cttaacttGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAAccc > 1:618722/1‑139 (MQ=255) gCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGt < 1:605312/80‑1 (MQ=255) gCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGt > 2:605312/1‑80 (MQ=255) cTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc < 2:618722/139‑1 (MQ=255) ttCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTcc < 1:848505/139‑1 (MQ=255) ttCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTcc < 2:600282/139‑1 (MQ=255) aTGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAaaaa < 2:539512/62‑1 (MQ=255) aTGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAaaaa > 1:539512/1‑62 (MQ=255) tGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCtata < 1:108873/139‑1 (MQ=255) tGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAgtagta > 2:1341470/1‑73 (MQ=255) tGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAgtagta < 1:1341470/73‑1 (MQ=255) tGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTc > 1:22071/1‑139 (MQ=255) ccGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTcc > 2:1270082/1‑122 (MQ=255) ccGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTcc < 1:1270082/122‑1 (MQ=255) aGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTAt < 1:1367018/68‑1 (MQ=255) aGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTAt > 2:1367018/1‑68 (MQ=255) aGCCGAATTAAGAAGAGAAATGTCGCACTCATAaaaaaa < 2:763914/39‑1 (MQ=255) aGCCGAATTAAGAAGAGAAATGTCGCACTCATAaaaaaa > 1:763914/1‑39 (MQ=255) aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc < 2:1610804/45‑1 (MQ=255) aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc > 1:1610804/1‑45 (MQ=255) aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGagag > 2:434254/1‑68 (MQ=255) aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGagag < 1:434254/68‑1 (MQ=255) ggAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTCGTATCGGTAATGAGAGCTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCa < 2:741692/138‑1 (MQ=255) aaTTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTtata > 2:1308377/1‑95 (MQ=255) aaTTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTtata < 1:1308377/95‑1 (MQ=255) aagaGAAATGTCGCACTCATAACAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTtata < 2:1153285/88‑1 (MQ=255) aagaGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTtata > 1:1153285/1‑88 (MQ=255) aagaGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCAt < 1:1414195/96‑1 (MQ=255) aagaGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCAt > 2:1414195/1‑96 (MQ=255) agaAATGTCGCACTCATAAAACAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACGAGTCAGGAGTCATTTCAGGATGCCATTTCGCGAAtcc > 1:741692/1‑138 (MQ=255) tCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTaa < 1:722603/70‑1 (MQ=255) tCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTaa > 2:722603/1‑70 (MQ=255) cTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAAcc > 1:1539077/1‑67 (MQ=255) cTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAAcc < 2:1539077/67‑1 (MQ=255) aTAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTcc > 2:921514/1‑76 (MQ=255) | CGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGC‑‑AGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCT > NC_000913/4604079‑4604322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |