Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,913	A→G	E104G (GAA→GGA)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,913	0	A	G	100.0%	211.9 / NA	58	E104G (GAA→GGA)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/32);  total (26/32)

TGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTT  >  NC_000913/4619784‑4620044                                                                                                                                  |                                                                                                                                    tGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCgg                                                                                                                            <  1:406868/139‑1 (MQ=255)   gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGgtg                                                                                                                          >  1:252029/1‑139 (MQ=255)     gAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTc                                                                                                                        <  2:716403/139‑1 (MQ=255)     gAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTc                                                                                                                        <  1:551175/139‑1 (MQ=255)               gAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGt                                                                                                              <  2:1029659/139‑1 (MQ=255)                       tACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTgg                                                                                                      <  1:1093861/139‑1 (MQ=255)                       tACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTgg                                                                                                      >  1:1069533/1‑139 (MQ=255)                       tACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTgg                                                                                                      >  2:847638/1‑139 (MQ=255)                            gTTTCATACAGGCGGGAACGGGCGGAAACGCTGAAGTTATGTGCGCGTACGCATGGGCGCACGAAATGTCATCCAGTAAAGATACCCCGTGTGGTCACTGGGGAATTGCCGATGTCACGGTTCTGTTTGAGTGGGGata                                                                                                 <  2:296019/139‑1 (MQ=255)                             tttCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGatat                                                                                                >  2:404976/1‑139 (MQ=255)                                 aTTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCgg                                                                                                                            >  2:424734/1‑107 (MQ=255)                                 aTTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCgg                                                                                                                            <  1:424734/107‑1 (MQ=255)                                      ggcggGAATGGACGGCAACGCTGAGGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGAt                                                                                       >  1:514674/1‑139 (MQ=255)                                                     cAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCgg                                                                                                                            <  2:487202/87‑1 (MQ=255)                                                     cAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCgg                                                                                                                            >  1:487202/1‑87 (MQ=255)                                                     cAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGt                                                                                                        <  2:1378109/107‑1 (MQ=255)                                                     cAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGt                                                                                                        >  1:1378109/1‑107 (MQ=255)                                                     cAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTtctc                                                                                           >  1:238633/1‑120 (MQ=255)                                                     cAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTtctc                                                                                           <  2:238633/120‑1 (MQ=255)                                                     cAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAACGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAg                                                                                                         >  1:1288542/1‑106 (MQ=255)                                                     cAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAACGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAg                                                                                                         <  2:1288542/106‑1 (MQ=255)                                                        cGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGc                                                                                                                               <  2:604315/81‑1 (MQ=255)                                                        cGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGc                                                                                                                               >  1:604315/1‑81 (MQ=255)                                                            gAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAag                                                                 <  2:1571740/139‑1 (MQ=255)                                                                ttATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgct                                                             <  2:252029/139‑1 (MQ=255)                                                                  aTCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGtt                                                                                                             <  1:494740/89‑1 (MQ=255)                                                                  aTCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGtt                                                                                                             >  2:494740/1‑89 (MQ=255)                                                                  aTCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctgc                                                           >  1:1171954/1‑139 (MQ=255)                                                                   tCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctgct                                                          <  2:1069533/139‑1 (MQ=255)                                                                             cGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTcc                                                                                                                       >  1:401444/1‑68 (MQ=255)                                                                             cGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTcc                                                                                                                       <  2:401444/68‑1 (MQ=255)                                                                                  gggCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGa                                           <  1:404976/139‑1 (MQ=255)                                                                                   ggCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                            >  2:1443392/1‑121 (MQ=255)                                                                                   ggCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                            <  1:1443392/121‑1 (MQ=255)                                                                                   ggCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGaa                                          <  2:514674/139‑1 (MQ=255)                                                                                     cgcACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAAcg                                        <  1:1178385/139‑1 (MQ=255)                                                                                       cACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGa                                           <  2:756050/134‑1 (MQ=255)                                                                                       cACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGa                                           >  1:756050/1‑134 (MQ=255)                                                                                                        tAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTAc                     >  2:1398400/1‑139 (MQ=255)                                                                                                         aaaGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTAcc                    <  1:847638/139‑1 (MQ=255)                                                                                                             aTACCCCGTCTGGTCACTGGGGAATTGCCGGTGTcc                                                                                                                       <  2:714978/36‑1 (MQ=255)                                                                                                             aTACCCCGTCTGGTCACTGGGGAATTGCCGGTGTcc                                                                                                                       >  1:714978/1‑36 (MQ=255)                                                                                                                ccccGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGt                                                                                                        >  2:111146/1‑48 (MQ=255)                                                                                                                ccccGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGt                                                                                                        <  1:111146/48‑1 (MQ=255)                                                                                                                    gTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGt                                                                                                        >  1:1144609/1‑44 (MQ=255)                                                                                                                    gTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGt                                                                                                        <  2:1144609/44‑1 (MQ=255)                                                                                                                    gTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGc         >  1:997894/1‑139 (MQ=255)                                                                                                                     tCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGtt                                                                                                             >  1:1261513/1‑38 (MQ=255)                                                                                                                     tCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGtt                                                                                                             <  2:1261513/38‑1 (MQ=255)                                                                                                                      cTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa       <  2:1171954/139‑1 (MQ=255)                                                                                                                      cTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa       >  2:819978/1‑139 (MQ=255)                                                                                                                        ggTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATc                                                                                      <  1:830147/58‑1 (MQ=255)                                                                                                                        ggTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATc                                                                                      >  2:830147/1‑58 (MQ=255)                                                                                                                           cACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACTAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCtt  <  2:997894/139‑1 (MQ=255)                                                                                                                            aCTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgct                                                             <  2:1607013/79‑1 (MQ=255)                                                                                                                            aCTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgct                                                             >  1:1607013/1‑79 (MQ=255)                                                                                                                              tGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAagag                                                               <  1:948834/75‑1 (MQ=255)                                                                                                                              tGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAagag                                                               >  2:948834/1‑75 (MQ=255)                                                                                                                                  |                                                                                                                                    TGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTT  >  NC_000913/4619784‑4620044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑