Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	383,259	C→T	R226Q (CGG→CAG)	yaiP ←	putative family 2 glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	383,259	0	C	T	100.0%	215.3 / NA	66	R226Q (CGG→CAG)	yaiP	putative family 2 glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/35);  total (31/35)

CCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCG  >  NC_000913/383124‑383393                                                                                                                                        |                                                                                                                                        ccGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGaa                                                                                                                                      <  1:1585386/139‑1 (MQ=255) ccGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGaa                                                                                                                                      <  2:731480/139‑1 (MQ=255)             aataaaAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGt                                                                                                                          <  1:63374/139‑1 (MQ=255)               taaaAGTCTTTTATGCATGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCCCTCCTCACGCGGGCTGTTGCATTCCTGTGGGGATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGtta                                                                                                                        <  1:1038964/139‑2 (MQ=255)                      cTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 <  1:238347/139‑1 (MQ=255)                      cTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 <  1:1102817/139‑1 (MQ=255)                      cTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 <  1:1279858/139‑1 (MQ=255)                        tttATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAAt                                                                                                               >  1:849357/1‑139 (MQ=255)                         ttATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 >  1:500825/1‑136 (MQ=255)                         ttATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 <  2:500825/136‑1 (MQ=255)                                           aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGt                                                                                           >  1:1267391/1‑139 (MQ=255)                                               cgTATCCCACAGTCCAGCGCCGCCAGCGACGCCACTCCTCACGCGGGTTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTc                                                                                       <  2:1267391/139‑1 (MQ=255)                                               cgTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCtt                                                                                        >  2:410099/1‑138 (MQ=255)                                               cgTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCtt                                                                                        <  1:410099/138‑1 (MQ=255)                                                             cAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 >  2:37658/1‑100 (MQ=255)                                                             cAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 <  1:37658/100‑1 (MQ=255)                                                              aaCGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATc                                                                        >  2:240741/1‑139 (MQ=255)                                                                     ccAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTc                                                                                                            <  2:1016921/97‑1 (MQ=255)                                                                     ccAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTc                                                                                                            >  1:1016921/1‑97 (MQ=255)                                                                     ccAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACt                                                                                   <  1:920679/121‑1 (MQ=255)                                                                     ccAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACt                                                                                   >  2:920679/1‑121 (MQ=255)                                                                              ccACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTa                                                        <  2:849357/139‑1 (MQ=255)                                                                              ccACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTa                                                        <  2:890639/139‑1 (MQ=255)                                                                                    ctcACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTc                                                                                                            >  1:1669811/1‑82 (MQ=255)                                                                                    ctcACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTc                                                                                                            <  2:1669811/82‑1 (MQ=255)                                                                                      cACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 >  1:956109/1‑75 (MQ=255)                                                                                      cACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 <  2:956109/75‑1 (MQ=255)                                                                                              cTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTAc                                        >  2:937587/1‑139 (MQ=255)                                                                                                       tCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATcc                               >  2:236140/1‑139 (MQ=255)                                                                                                        ccTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATccc                              <  1:236140/139‑1 (MQ=255)                                                                                                         cTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTc                                                                                                            >  1:1345315/1‑61 (MQ=255)                                                                                                         cTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTc                                                                                                            <  2:1345315/61‑1 (MQ=255)                                                                                                          tgtgGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGc                            <  1:937587/139‑1 (MQ=255)                                                                                                           gtgGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTa                                                        <  1:1108163/110‑1 (MQ=255)                                                                                                           gtgGGTAGACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GATGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTa                                                        >  2:1108163/1‑110 (MQ=255)                                                                                                             gggTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAgg                         <  1:240741/139‑1 (MQ=255)                                                                                                                 ataCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGt                                                                                                      >  1:1440080/1‑58 (MQ=255)                                                                                                                 ataCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGt                                                                                                      <  2:1440080/58‑1 (MQ=255)                                                                                                                 ataCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCtt                                                                                        >  1:496554/1‑72 (MQ=255)                                                                                                                 ataCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCtt                                                                                        <  2:496554/72‑1 (MQ=255)                                                                                                                  taCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCg                    >  2:1280418/1‑139 (MQ=255)                                                                                                                   aCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTa                                         <  1:1619680/118‑1 (MQ=255)                                                                                                                   aCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTa                                         >  2:1619680/1‑118 (MQ=255)                                                                                                                   aCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGc                   <  1:1280418/139‑1 (MQ=255)                                                                                                                        gCAGCGATTCGCCTGTTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCtt                                                                                        <  1:1559257/65‑1 (MQ=255)                                                                                                                        gCAGCGATTCGCCTGTTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCtt                                                                                        >  2:1559257/1‑65 (MQ=255)                                                                                                                         cAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGGCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGataa             >  2:1275243/1‑139 (MQ=255)                                                                                                                          aGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACtt                                                                                  <  2:1024761/69‑1 (MQ=255)                                                                                                                          aGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACtt                                                                                  >  1:1024761/1‑69 (MQ=255)                                                                                                                          aGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCa                       <  2:1057612/128‑1 (MQ=255)                                                                                                                          aGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCa                       >  1:1057612/1‑128 (MQ=255)                                                                                                                          aGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCg                    >  2:475446/1‑131 (MQ=255)                                                                                                                          aGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCg                    <  1:475446/131‑1 (MQ=255)                                                                                                                          aGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCg                    <  1:418111/131‑1 (MQ=255)                                                                                                                          aGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGataat            >  2:1361276/1‑139 (MQ=255)                                                                                                                          aGCGATTCGCCAGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCg                    >  2:418111/1‑131 (MQ=255)                                                                                                                            cGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACt                                                                                   <  2:1000077/66‑1 (MQ=255)                                                                                                                            cGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACt                                                                                   >  1:1000077/1‑66 (MQ=255)                                                                                                                              tttCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTc                                                                                       >  2:1058241/2‑60 (MQ=255)                                                                                                                              aTTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑TGTGAGATCAAGGTCTTc                                                                                       <  1:1058241/60‑1 (MQ=255)                                                                                                                              aTTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGAc                                                                                    >  2:470044/1‑63 (MQ=255)                                                                                                                              aTTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGAc                                                                                    <  1:470044/63‑1 (MQ=255)                                                                                                                               ttCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCtt                                                                                        <  1:1316083/58‑1 (MQ=255)                                                                                                                               ttCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCtt                                                                                        >  2:1316083/1‑58 (MQ=255)                                                                                                                                 cGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGt     >  2:794787/1‑139 (MQ=255)                                                                                                                                 cGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGt     >  1:999027/1‑139 (MQ=255)                                                                                                                                    ctgctgAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTgcg  <  1:1275243/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCA‑GGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCG  >  NC_000913/383124‑383393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑