Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	443,604	C→T	S13N (AGT→AAT)  *304* (TAG→TAA)	yajL ← panE ←	oxidative‑stress‑resistance chaperone 2‑dehydropantoate reductase, NADPH‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	443,604	0	C	T	100.0%	119.7 / NA	38	S13N (AGT→AAT)  *304* (TAG→TAA)	yajL panE	oxidative‑stress‑resistance chaperone 2‑dehydropantoate reductase, NADPH‑specific
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/19);  total (19/19)

CTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGT  >  NC_000913/443486‑443721                                                                                                                       |                                                                                                                      cTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAAcc                                                                                                   >  2:1169833/1‑139 (MQ=255)           cgcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                         >  1:514091/1‑139 (MQ=255)            gcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATgc                                                                                        <  2:688924/139‑1 (MQ=255)               aGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCt                                                                                     <  1:1169833/139‑1 (MQ=255)                   ggTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCata                                                                                 >  2:167166/1‑139 (MQ=255)                              ggTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCATTGCCGATg                                                                                         <  1:1098584/120‑1 (MQ=255)                              ggTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                         >  2:1098584/1‑120 (MQ=255)                              ggTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCct                                                                      <  2:514091/139‑1 (MQ=255)                                        gCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTAc                                                            >  2:136288/1‑139 (MQ=255)                                             cGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAgggg                                                                                                              >  1:1527628/1‑84 (MQ=255)                                             cGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAgggg                                                                                                              <  2:1527628/84‑1 (MQ=255)                                                               cTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGtttt                                     >  1:810134/1‑139 (MQ=255)                                                                    aTACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                         >  1:643739/1‑82 (MQ=255)                                                                    aTACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                         <  2:643739/82‑1 (MQ=255)                                                                        cgccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAgggg                                                                                                              <  1:1514266/57‑1 (MQ=255)                                                                        cgccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAgggg                                                                                                              >  2:60388/1‑57 (MQ=255)                                                                        cgccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAgggg                                                                                                              <  1:60388/57‑1 (MQ=255)                                                                        cgccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAgggg                                                                                                              >  2:1514266/1‑57 (MQ=255)                                                                          ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAgggg                                                                                                              >  1:1332361/1‑55 (MQ=255)                                                                          ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAgggg                                                                                                              <  2:1332361/55‑1 (MQ=255)                                                                               aaCCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCAct                                                                           >  1:1090979/1‑85 (MQ=255)                                                                               aaCCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCAct                                                                           <  2:1090979/85‑1 (MQ=255)                                                                                 ccAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCAt                   <  2:810134/139‑1 (MQ=255)                                                                                          cGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATccc                     <  2:1239354/128‑1 (MQ=255)                                                                                          cGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATccc                     >  1:1239354/1‑128 (MQ=255)                                                                                          cGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcggg          >  2:1065414/1‑139 (MQ=255)                                                                                            aTAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcgggcg        <  2:887560/139‑1 (MQ=255)                                                                                            aTAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcgggcg        <  1:1489472/139‑1 (MQ=255)                                                                                                  gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATgcg                                                                                       >  1:1141677/1‑54 (MQ=255)                                                                                                  gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATgcg                                                                                       <  2:1141677/54‑1 (MQ=255)                                                                                                       ggCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcgggcg        <  1:1062691/128‑1 (MQ=255)                                                                                                       ggCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcgggcg        >  2:1062691/1‑128 (MQ=255)                                                                                                            cAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCt                                                                                     >  1:720334/1‑46 (MQ=255)                                                                                                            cAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCt                                                                                     <  2:720334/46‑1 (MQ=255)                                                                                                            cAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGt  <  1:839788/129‑1 (MQ=255)                                                                                                            cAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGt  >  2:839788/1‑129 (MQ=255)                                                                                                                   ttCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAgg                                               >  1:1490057/1‑77 (MQ=255)                                                                                                                   ttCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAgg                                               <  2:1490057/77‑1 (MQ=255)                                                                                                                       |                                                                                                                      CTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGT  >  NC_000913/443486‑443721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑