Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	470,643	G→A	*591* (TAG→TAA)  S3N (AGT→AAT)	mdlA → mdlB →	putative multidrug ABC transporter ATPase putative multidrug ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	470,643	0	G	A	100.0%	167.9 / NA	52	*591* (TAG→TAA)  S3N (AGT→AAT)	mdlA mdlB	putative multidrug ABC transporter ATPase putative multidrug ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/28);  total (24/28)

TCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCA  >  NC_000913/470513‑470774                                                                                                                                   |                                                                                                                                    tCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGcc                                                                                                                             <  2:1622700/139‑1 (MQ=255)    cccAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAAc                                                                                                                          >  2:854342/1‑139 (MQ=255)            ggCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACt                                                                                                                         >  2:621448/1‑132 (MQ=255)            ggCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACt                                                                                                                         <  1:621448/132‑1 (MQ=255)            ggCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCg                                                                                                                  >  2:345472/1‑139 (MQ=255)                     gatgTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAg                                                                                                         <  1:854342/139‑1 (MQ=255)                                 caacaaAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGt                                                                                                  <  2:234456/134‑1 (MQ=255)                                 caacaaAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGt                                                                                                  >  1:234456/1‑134 (MQ=255)                                       aGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGt                                                                                       <  1:1335876/139‑1 (MQ=255)                                        gCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGTCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGtt                                                                                      >  2:1070541/1‑139 (MQ=255)                                            cTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                                  >  2:934082/1‑139 (MQ=255)                                             tGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCg                                                                                 <  1:842008/139‑1 (MQ=255)                                                    gcgATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTa                                                                          <  1:1070541/139‑1 (MQ=255)                                                    gcgATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTa                                                                          <  2:668152/139‑1 (MQ=255)                                                     cgATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCaa                                                                                                                           <  1:557274/89‑1 (MQ=255)                                                     cgATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCaa                                                                                                                           >  2:557274/1‑89 (MQ=255)                                                          tGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACtctc                                                                                                            <  2:270718/99‑1 (MQ=255)                                                          tGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACtctc                                                                                                            >  1:270718/1‑99 (MQ=255)                                                             aTCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGcc                                                                                                     <  1:105054/103‑1 (MQ=255)                                                             aTCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGcc                                                                                                     >  2:105054/1‑103 (MQ=255)                                                               cGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                                  >  1:1441147/1‑120 (MQ=255)                                                               cGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                                  <  2:1441147/120‑1 (MQ=255)                                                                   aTCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTg                                                           <  1:934082/139‑1 (MQ=255)                                                                   aTCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTg                                                           <  2:656598/139‑1 (MQ=255)                                                                    tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                                  >  1:117012/1‑115 (MQ=255)                                                                    tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                                  <  2:117012/115‑1 (MQ=255)                                                                    tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGc                                                          >  2:91821/1‑139 (MQ=255)                                                                    tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGc                                                          >  2:580473/1‑139 (MQ=255)                                                                      aacaacTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGcc                                                                                                                   <  1:590613/80‑1 (MQ=255)                                                                      aacaacTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGcc                                                                                                                   >  2:590613/1‑80 (MQ=255)                                                                      aacaacTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCg                                                                     <  1:780063/126‑1 (MQ=255)                                                                      aacaacTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCg                                                                     >  2:780063/1‑126 (MQ=255)                                                                      aacaacTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCgg                                                        <  1:580473/139‑1 (MQ=255)                                                                           cTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCtg                                                   <  1:91821/139‑1 (MQ=255)                                                                              gAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCTGTCGATGCGAAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTaaa                                                                        >  2:1041693/1‑115 (MQ=255)                                                                              gAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTaaa                                                                        <  1:1041693/115‑1 (MQ=255)                                                                                   ggcgCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCaa                                                                                                                           <  1:732315/59‑1 (MQ=255)                                                                                   ggcgCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCaa                                                                                                                           >  2:732315/1‑59 (MQ=255)                                                                                   ggcgCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGAtgtg                                           >  1:457851/1‑139 (MQ=255)                                                                                     cgcTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGAc                                                                                                                <  2:646318/68‑1 (MQ=255)                                                                                     cgcTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGAc                                                                                                                >  1:646318/1‑68 (MQ=255)                                                                                                  tCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCggcggcggc                            >  1:245593/1‑139 (MQ=255)                                                                                                     ggAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGa                         <  2:1533767/139‑1 (MQ=255)                                                                                                              cgAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTgg                >  2:330906/1‑139 (MQ=255)                                                                                                                aggaggCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCtg                                                   >  1:1101202/1‑102 (MQ=255)                                                                                                                aggaggCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCtg                                                   <  2:1101202/102‑1 (MQ=255)                                                                                                                aggaggCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGatgatg                                             <  2:72393/108‑1 (MQ=255)                                                                                                                aggaggCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGatgatg                                             >  1:72393/1‑108 (MQ=255)                                                                                                                aggaggCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGc              <  2:1611291/139‑1 (MQ=255)                                                                                                                       cGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGcggcggcgg                             <  2:529353/117‑1 (MQ=255)                                                                                                                       cGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGcggcggcgg                             >  1:529353/1‑117 (MQ=255)                                                                                                                            aTGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCa  <  2:245593/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                    TCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCA  >  NC_000913/470513‑470774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑