Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 675,601 Δ1 bp coding (947/978 nt) ybeQ ← Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913675,6010T.100.0% 285.2 / NA 66coding (947/978 nt)ybeQSel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (33/33);  total (33/33)

TGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCGTCATTGC  >  NC_000913/675467‑675730
                                                                                                                                      |                                                                                                                                 
tGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTccgactg                                                                                                                              >  1:612528/1‑139 (MQ=255)
            gagaCGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGtt                                                                                                                  >  2:757627/1‑139 (MQ=255)
            gagaCGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGtt                                                                                                                  >  2:417903/1‑139 (MQ=255)
                 gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGa                                                                                                             >  1:103812/1‑139 (MQ=255)
                 gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGa                                                                                                             >  1:727062/1‑139 (MQ=255)
                 gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGa                                                                                                             >  2:507914/1‑139 (MQ=255)
                 gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCACGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGa                                                                                                             >  2:500811/1‑139 (MQ=255)
                      tttGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACt                                                                                                        >  2:661394/1‑139 (MQ=255)
                         gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                     <  1:661394/139‑1 (MQ=255)
                         gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                     <  1:330435/139‑1 (MQ=255)
                         gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                     <  2:705971/139‑1 (MQ=255)
                                 aTAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGa                                                                                             >  2:320707/1‑139 (MQ=255)
                                 aTAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGa                                                                                             <  1:500811/139‑1 (MQ=255)
                                         aTTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTg                                                                                                       >  2:194102/1‑121 (MQ=255)
                                         aTTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTg                                                                                                       <  1:194102/121‑1 (MQ=255)
                                         aTTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                     >  2:541886/1‑123 (MQ=255)
                                         aTTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                     <  1:541886/123‑1 (MQ=255)
                                         aTTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTc                                                                                     >  2:81079/1‑139 (MQ=255)
                                               cacaGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGc                                                                               <  1:757627/139‑1 (MQ=255)
                                               cacaGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGc                                                                               <  1:417903/139‑1 (MQ=255)
                                                   gCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACAtct                                                                                                                        <  2:14116/94‑1 (MQ=255)
                                                   gCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACAtct                                                                                                                        >  1:14116/1‑94 (MQ=255)
                                                   gCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGagag                                                                                <  2:362129/134‑1 (MQ=255)
                                                   gCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGagag                                                                                >  1:362129/1‑134 (MQ=255)
                                                   gCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGt                                                                           >  2:372265/1‑139 (MQ=255)
                                                   gCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGt                                                                           >  2:671407/1‑139 (MQ=255)
                                                              aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                                >  2:116527/1‑139 (MQ=255)
                                                              aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                                >  2:320986/1‑139 (MQ=255)
                                                              aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                                >  1:113505/1‑139 (MQ=255)
                                                              aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                                >  1:134822/1‑139 (MQ=255)
                                                               atatGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACa                                                               <  1:591920/139‑1 (MQ=255)
                                                               atatGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACa                                                               <  1:320707/139‑1 (MQ=255)
                                                                 atGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAgg                                                             <  2:789681/139‑1 (MQ=255)
                                                                  tGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGt                                                            >  1:3564/1‑139 (MQ=255)
                                                                   tggCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCATCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTc                                                           <  1:172272/138‑1 (MQ=255)
                                                                   gggCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTc                                                           >  1:628411/1‑139 (MQ=255)
                                                                     gCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACt                                                                                                        >  1:458959/1‑92 (MQ=255)
                                                                     gCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACt                                                                                                        <  2:458959/92‑1 (MQ=255)
                                                                      ccGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttc                                                        >  2:605232/1‑139 (MQ=255)
                                                                        gAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcct                                                      >  2:606830/1‑139 (MQ=255)
                                                                        gAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcct                                                      >  2:558341/1‑139 (MQ=255)
                                                                           tGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                     >  1:375339/1‑89 (MQ=255)
                                                                           tGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                     <  2:375339/89‑1 (MQ=255)
                                                                              aaTTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                                                <  1:507914/139‑1 (MQ=255)
                                                                              aaTTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                                                <  2:612528/139‑1 (MQ=255)
                                                                                ttCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTa                                              <  2:381234/139‑1 (MQ=255)
                                                                                ttCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTa                                              <  2:134822/139‑1 (MQ=255)
                                                                                ttCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTa                                              <  1:116527/139‑1 (MQ=255)
                                                                                 tCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAg                                             <  1:372265/139‑1 (MQ=255)
                                                                                 tCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAg                                             <  1:264233/139‑1 (MQ=255)
                                                                                     cGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGAt                                         >  2:382000/1‑139 (MQ=255)
                                                                                     cGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGAt                                         >  2:532295/1‑139 (MQ=255)
                                                                                            tGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAAt                                  >  2:376727/1‑139 (MQ=255)
                                                                                               ttttATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc                               <  1:532295/139‑1 (MQ=255)
                                                                                               ttttATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc                               <  1:320986/139‑1 (MQ=255)
                                                                                               ttttATCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc                               <  2:34038/139‑1 (MQ=255)
                                                                                                   aTCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg                           <  1:671407/139‑1 (MQ=255)
                                                                                                   aTCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg                           <  1:606830/139‑1 (MQ=255)
                                                                                                   aTCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg                           <  2:628411/139‑1 (MQ=255)
                                                                                                   aTCTTTACAGTAAATCTTCAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGACTTCCTTCTCCGTAGTGATACATAATGGCCAgg                           <  2:103812/139‑1 (MQ=255)
                                                                                                                     cAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACa                                                               <  2:349711/85‑1 (MQ=255)
                                                                                                                     cAGTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACa                                                               >  1:349711/1‑85 (MQ=255)
                                                                                                                       gTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCga                                                                                   <  1:476892/63‑1 (MQ=255)
                                                                                                                       gTCTCACGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCga                                                                                   >  2:476892/1‑63 (MQ=255)
                                                                                                                            aCGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGc                                                                               >  1:512681/1‑62 (MQ=255)
                                                                                                                            aCGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGc                                                                               <  2:512681/62‑1 (MQ=255)
                                                                                                                            aCGAATTTCCGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCGTCATTGc  >  1:456749/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                 
TGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCGTCATTGC  >  NC_000913/675467‑675730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: