Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,089,069	(T)7→6	coding (789/2019 nt)	pgaB ←	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,089,063	0	T	.	100.0%	142.2 / NA	34	coding (795/2019 nt)	pgaB	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/18);  total (16/18)

TTTTCCTGTACGGTAATAATTTGCTGGGCAAACTCTTTTAATGAGGGATTATTGGCGATTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAGTTTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCC  >  NC_000913/1088926‑1089190                                                                                                                                          |                                                                                                                                ttttCCTGTACGGTAATAATTTGCTGGGCAAACTCTTTTAATGAGGGATTATTGGCGATTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAGtt                                                                                                                                >  1:22185/1‑139 (MQ=255)              taataaTTTGCTGGGCAAACTCTTTTAATGAGGGATTATTGGCGATTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCctct                                                                                                                  >  1:131742/1‑139 (MQ=255)                    tttGCTGGGCAAACTCTTTTAATGAGGGATTATTGGCGATTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCt                                                                                                            <  2:560247/139‑1 (MQ=255)                               aaCTCTTTTAATGAGGGATTATTGGCGATTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCAttcgc                                                                                                 >  1:271956/1‑139 (MQ=255)                                  tctTTTAATGAGGGATTATTGGCGATTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCAttcgcttc                                                                                              <  1:550434/139‑1 (MQ=255)                                      ttATTGAGGGATTATTGTCTATTAATACCCGCGTAATGGAATCCAATTGCGACGCATTTGCGAAACCTGATTCAAGGTTGAAGAACATGACATAACCGAG‑TTTGTTAGTTCCTGTATCGCTATTCCATTCGCTTCGCCa                                                                                          <  1:432355/139‑1 (MQ=255)                                        aaTGAGGGATTATTGGCGATTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATa                                                                                        <  2:131742/139‑1 (MQ=255)                                             gggATTATTGGCGATTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGcc                                                                                   <  2:271956/139‑1 (MQ=255)                                              ggATTATTGGCGATTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCca                                                                                  >  1:428896/1‑139 (MQ=255)                                                attattGGCGATTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCAt                                                                                         <  2:398121/130‑1 (MQ=255)                                                attattGGCGATTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCAt                                                                                         >  1:398121/1‑130 (MQ=255)                                                         gATTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACgt                                                                       <  2:720151/139‑1 (MQ=255)                                                          aTTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACgtg                                                                      <  2:428896/139‑1 (MQ=255)                                                          aTTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACgtg                                                                      >  2:205063/1‑139 (MQ=255)                                                               tACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGAt                                                                 <  1:558577/139‑1 (MQ=255)                                                               tACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGAt                                                                 <  2:22185/139‑1 (MQ=255)                                                                     cgGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCt                                                           >  2:609563/1‑139 (MQ=255)                                                                     cgGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCt                                                           >  2:306367/1‑139 (MQ=255)                                                                            gaatCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAAC‑‑‑‑GGATTTACCTCAACCTTTGTa                                                <  2:27670/139‑1 (MQ=255)                                                                                ccAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTa                                                <  1:362796/139‑1 (MQ=255)                                                                                  aaTTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACg                                              >  2:664611/1‑139 (MQ=255)                                                                                                tGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTCCGTCa                                <  1:664611/139‑1 (MQ=255)                                                                                                     aaCCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTCCGTCAttttt                           >  1:339498/1‑139 (MQ=255)                                                                                                      aCCTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTa                          >  2:17435/1‑139 (MQ=255)                                                                                                        cTGATTCAAGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACa                        <  1:638634/139‑1 (MQ=255)                                                                                                               aaGGGTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCa                 >  2:325383/1‑139 (MQ=255)                                                                                                                 gggTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTcc                                    >  1:389683/1‑118 (MQ=255)                                                                                                                 gggTGAAGAACATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTcc                                    <  2:389683/118‑1 (MQ=255)                                                                                                                          aCATGTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCtt      <  1:17435/139‑1 (MQ=255)                                                                                                                              gTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCt                                                           <  2:346039/82‑1 (MQ=255)                                                                                                                              gTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCt                                                           >  1:346039/1‑82 (MQ=255)                                                                                                                              gTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTccc  >  2:297858/1‑139 (MQ=255)                                                                                                                              gTCATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTccc  >  2:772281/1‑139 (MQ=255)                                                                                                                                cATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCt                                                     >  1:9537/1‑86 (MQ=255)                                                                                                                                cATAACCGAG‑TTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCt                                                     <  2:9537/86‑1 (MQ=255)                                                                                                                                          |                                                                                                                                TTTTCCTGTACGGTAATAATTTGCTGGGCAAACTCTTTTAATGAGGGATTATTGGCGATTAATACCCGCGGAATGGAATCCAATTGCGACGCATTTGCCAAACCTGATTCAAGGGTGAAGAACATGTCATAACCGAGTTTTTTTAATTCCTCTATCGCTATGCCATTCGCTTCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCC  >  NC_000913/1088926‑1089190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑