Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,869,022 (T)6→5 coding (68/1284 nt) yeaH → UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,869,0170T.100.0% 63.1 / NA 16coding (63/1284 nt)yeaHUPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/8);  total (8/8)

ACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGG  >  NC_000913/1868882‑1869154
                                                                                                                                       |                                                                                                                                         
aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttt                                                                                                                                        >  1:401361/1‑139 (MQ=255)
  aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                      <  2:62974/139‑2 (MQ=255)
  aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                      <  2:513641/139‑2 (MQ=255)
                        cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATTTCCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                      <  1:492563/117‑2 (MQ=255)
                        cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGTCCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                      >  2:492563/1‑116 (MQ=255)
                        cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                      >  1:405149/1‑116 (MQ=255)
                        cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                      <  2:405149/117‑2 (MQ=255)
                                caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                       <  1:470294/139‑1 (MQ=255)
                                         cATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacgc                                                                                                                                   <  2:749049/103‑5 (MQ=255)
                                         cATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacgc                                                                                                                                   >  1:749049/1‑99 (MQ=255)
                                                  ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                     >  2:158487/1‑139 (MQ=255)
                                                   gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                                    <  1:756218/92‑4 (MQ=255)
                                                   gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                                    >  2:756218/1‑89 (MQ=255)
                                                          tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc                                                                             >  1:560588/1‑139 (MQ=255)
                                                                       atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTc                                                                >  1:754031/1‑139 (MQ=255)
                                                                                ggTTTATTGACCGGCGTTTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                             <  2:427805/101‑1 (MQ=255)
                                                                                ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                             >  1:427805/1‑101 (MQ=255)
                                                                                   ttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGaa                                                    <  1:627018/139‑1 (MQ=255)
                                                                                     aTTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCACCGAGGCCATTAATAAGCGTTCGGTTACTGACGTCGACAGCGGCGAATc                                                  >  2:129809/1‑139 (MQ=255)
                                                                                           cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAgcgc                                                                                                                     <  1:569110/66‑1 (MQ=255)
                                                                                           cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAgcgc                                                                                                                     >  2:569110/1‑66 (MQ=255)
                                                                                           cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                      <  1:494208/129‑1 (MQ=255)
                                                                                           cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                            >  1:228431/1‑139 (MQ=255)
                                                                                           aggCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAGCAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                      >  2:494208/2‑129 (MQ=255)
                                                                                            ggCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATcc                                           <  2:754031/139‑1 (MQ=255)
                                                                                              cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                                         <  1:158487/139‑1 (MQ=255)
                                                                                               gTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAtt                                        <  2:560588/139‑1 (MQ=255)
                                                                                                                                      cttttttACCCCGTTATAAAGCTCAAATTAATCAGTCGATCTCTGAGGCCATTATTAAGCGTTCGTTGACTGACTTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAggg  <  1:129809/138‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                         
ACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGG  >  NC_000913/1868882‑1869154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: