Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,200,454 (A)8→7 coding (176/3633 nt) yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,200,4470A.100.0% 85.4 / NA 21coding (169/3633 nt)yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (10/11);  total (10/11)

AACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCG  >  NC_000913/2200309‑2200566
                                                                                                                                          |                                                                                                                       
aaCGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTTGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGagaaga                                                                                                                         >  2:374368/1‑139 (MQ=255)
                              ggCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCCTCCAGAAATTGGGGGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAAttt                                                                                          <  2:314899/139‑1 (MQ=255)
                               gCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAAtttt                                                                                         >  1:760487/1‑139 (MQ=255)
                                     cgccgcTCAGTCATCGTCGCTGGCCGGGCGAAGCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTg                                                                                   <  2:687803/139‑1 (MQ=255)
                                           tCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTgg                                                                             <  2:791309/139‑1 (MQ=255)
                                                            ccGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa                                                            <  2:760487/139‑1 (MQ=255)
                                                            ccGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa                                                            <  2:359718/139‑1 (MQ=255)
                                                              gggCGAACCAGTGTCCGTTATCACTTGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAgg                                                          <  1:374368/139‑1 (MQ=255)
                                                                                                aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt                        >  1:1581/1‑139 (MQ=255)
                                                                                                aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt                        >  2:138043/1‑139 (MQ=255)
                                                                                                aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt                        >  2:175854/1‑139 (MQ=255)
                                                                                                     gACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                        >  1:30096/1‑86 (MQ=255)
                                                                                                     gACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                        <  2:30096/86‑1 (MQ=255)
                                                                                                     gACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccgg                   >  2:1883/1‑139 (MQ=255)
                                                                                                             gTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACa           <  1:737727/139‑1 (MQ=255)
                                                                                                                atgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCAt                                                      <  2:81613/93‑1 (MQ=255)
                                                                                                                atgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCAt                                                      >  1:81613/1‑93 (MQ=255)
                                                                                                                 tgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATgg       >  2:398824/1‑139 (MQ=255)
                                                                                                                      tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg  >  2:136134/1‑139 (MQ=255)
                                                                                                                      tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg  <  1:717611/139‑1 (MQ=255)
                                                                                                                           cAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                       >  2:253716/1‑113 (MQ=255)
                                                                                                                           cAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                       <  1:253716/113‑1 (MQ=255)
                                                                                                                                          |                                                                                                                       
AACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCG  >  NC_000913/2200309‑2200566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: