Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,014,259 Δ1 bp intergenic (‑20/+28) yqeB ← / ← yqeC XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold/putative selenium‑dependent hydroxylase accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,014,2580C.95.7% 87.8 / ‑2.4 23intergenic (‑19/+29)yqeB/yqeCXdhC‑CoxI family protein with NAD(P)‑binding Rossman fold/putative selenium‑dependent hydroxylase accessory protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (10/12);  minor base G (0/1);  total (10/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCG  >  NC_000913/3014137‑3014383
                                                                                                                         |                                                                                                                             
gtgCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAAt                                                                                                             >  2:198328/1‑139 (MQ=255)
                          cGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTg                                                                                   <  2:431394/139‑1 (MQ=255)
                          cGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTg                                                                                   >  1:431394/1‑139 (MQ=255)
                              tATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                               <  2:342770/139‑1 (MQ=255)
                                         tGCCCCATAGCAAACGGGCAATTTTGCTCTTCGATTTTAGCAGGCTTTGAGAAAATATTCTTCCCAACCTCCATGGATAGCTTTCAGTAACCCGGAATACCCAGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                    <  1:553539/139‑1 (MQ=255)
                                           cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                  <  2:84977/139‑1 (MQ=255)
                                            gcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                    >  1:596264/1‑136 (MQ=255)
                                            gcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                    <  2:596264/136‑1 (MQ=255)
                                               aTAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCt                                                              >  1:353954/1‑139 (MQ=255)
                                                    aaaCGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGtat                                                         <  2:683585/139‑1 (MQ=255)
                                                          aCAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                    >  2:578870/1‑122 (MQ=255)
                                                          aCAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                    <  1:578870/122‑1 (MQ=255)
                                                                    tctTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAg                                                                      <  1:70436/110‑1 (MQ=255)
                                                                    tctTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAg                                                                      >  2:70436/1‑110 (MQ=255)
                                                                        cGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCct                                     <  2:529557/139‑1 (MQ=255)
                                                                                gCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                               >  1:278844/1‑89 (MQ=255)
                                                                                gCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                               <  2:278844/89‑1 (MQ=255)
                                                                                    ccTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCAGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGtgtt                         <  1:767026/139‑1 (MQ=255)
                                                                                                      tACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGa       >  2:697945/1‑139 (MQ=255)
                                                                                                        ccAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGc     <  1:369194/139‑1 (MQ=255)
                                                                                                           aCCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCg  >  2:140314/1‑139 (MQ=255)
                                                                                                           aCCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCg  <  1:129825/139‑1 (MQ=255)
                                                                                                           aCCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACATCGTGTTGTTGCAGCGGTTGAAGGAGCTCg  >  2:569274/1‑139 (MQ=255)
                                                                                                                         |                                                                                                                             
GTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCG  >  NC_000913/3014137‑3014383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: